4WXV

Human cationic trypsin K97D mutant in complex with bovine pancreatic trypsin inhibitor (BPTI)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004252	molecular_function	serine-type endopeptidase activity
A	0005576	cellular_component	extracellular region
A	0005615	cellular_component	extracellular space
A	0006508	biological_process	proteolysis
A	0007586	biological_process	digestion
A	0008233	molecular_function	peptidase activity
A	0008236	molecular_function	serine-type peptidase activity
A	0016787	molecular_function	hydrolase activity
A	0022617	biological_process	extracellular matrix disassembly
A	0031012	cellular_component	extracellular matrix
A	0046872	molecular_function	metal ion binding
A	0072562	cellular_component	blood microparticle
B	0004252	molecular_function	serine-type endopeptidase activity
B	0005576	cellular_component	extracellular region
B	0005615	cellular_component	extracellular space
B	0006508	biological_process	proteolysis
B	0007586	biological_process	digestion
B	0008233	molecular_function	peptidase activity
B	0008236	molecular_function	serine-type peptidase activity
B	0016787	molecular_function	hydrolase activity
B	0022617	biological_process	extracellular matrix disassembly
B	0031012	cellular_component	extracellular matrix
B	0046872	molecular_function	metal ion binding
B	0072562	cellular_component	blood microparticle
C	0004867	molecular_function	serine-type endopeptidase inhibitor activity
I	0004867	molecular_function	serine-type endopeptidase inhibitor activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	binding site for residue CA A 301

Chain	Residue
A	GLU70
A	ASN72
A	VAL75
A	GLU77
A	GLU80

---

site_id	AC2
Number of Residues	6
Details	binding site for residue CA B 301

Chain	Residue
B	HOH404
B	HOH422
B	GLU70
B	ASN72
B	VAL75
B	GLU80

---

site_id	AC3
Number of Residues	3
Details	binding site for residue SO4 C 101

Chain	Residue
C	ARG20
C	TYR35
C	ALA40

---

site_id	AC4
Number of Residues	6
Details	binding site for residue SO4 C 102

Chain	Residue
C	ARG39
C	ALA40
I	PRO2
I	ASP3
I	PHE4
I	ARG42

---

site_id	AC5
Number of Residues	5
Details	binding site for residue SO4 I 101

Chain	Residue
B	ARG96
I	ARG20
I	TYR35
I	ALA40
I	HOH209

---

site_id	AC6
Number of Residues	6
Details	binding site for residue SO4 I 102

Chain	Residue
C	ARG42
I	GLU7
I	LYS41
I	ARG42
I	HOH201
I	HOH203

---

Functional Information from PROSITE/UniProt

site_id	PS00134
Number of Residues	6
Details	TRYPSIN_HIS Serine proteases, trypsin family, histidine active site. VSAGHC

Chain	Residue	Details
A	VAL53-CYS58

---

site_id	PS00280
Number of Residues	19
Details	BPTI_KUNITZ_1 Pancreatic trypsin inhibitor (Kunitz) family signature. FvyGGCrakrnnFksaedC

Chain	Residue	Details
C	PHE33-CYS51

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	440
Details	Domain: {"description":"Peptidase S1","evidences":[{"source":"PROSITE-ProRule","id":"PRU00274","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	Active site: {"description":"Charge relay system"}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	Binding site: {}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Site: {"description":"Required for specificity","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	Modified residue: {"description":"Sulfotyrosine","evidences":[{"source":"PubMed","id":"17087724","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"25010489","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	100
Details	Domain: {"description":"BPTI/Kunitz inhibitor","evidences":[{"source":"PROSITE-ProRule","id":"PRU00031","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	Site: {"description":"Reactive bond for trypsin"}

Chain

Residue

Details

---