4WNC

Crystal structure of human wild-type GAPDH at 1.99 angstroms resolution

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000226	biological_process	microtubule cytoskeleton organization
A	0001819	biological_process	positive regulation of cytokine production
A	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005811	cellular_component	lipid droplet
A	0005829	cellular_component	cytosol
A	0005856	cellular_component	cytoskeleton
A	0005886	cellular_component	plasma membrane
A	0006006	biological_process	glucose metabolic process
A	0006096	biological_process	glycolytic process
A	0006417	biological_process	regulation of translation
A	0006915	biological_process	apoptotic process
A	0008017	molecular_function	microtubule binding
A	0010951	biological_process	negative regulation of endopeptidase activity
A	0015630	cellular_component	microtubule cytoskeleton
A	0016020	cellular_component	membrane
A	0016241	biological_process	regulation of macroautophagy
A	0016491	molecular_function	oxidoreductase activity
A	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
A	0016740	molecular_function	transferase activity
A	0017148	biological_process	negative regulation of translation
A	0019828	molecular_function	aspartic-type endopeptidase inhibitor activity
A	0031640	biological_process	killing of cells of another organism
A	0031965	cellular_component	nuclear membrane
A	0031982	cellular_component	vesicle
A	0032481	biological_process	positive regulation of type I interferon production
A	0035605	molecular_function	peptidyl-cysteine S-nitrosylase activity
A	0035606	biological_process	peptidyl-cysteine S-trans-nitrosylation
A	0042802	molecular_function	identical protein binding
A	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
A	0043231	cellular_component	intracellular membrane-bounded organelle
A	0045087	biological_process	innate immune response
A	0048471	cellular_component	perinuclear region of cytoplasm
A	0050661	molecular_function	NADP binding
A	0050821	biological_process	protein stabilization
A	0050832	biological_process	defense response to fungus
A	0051287	molecular_function	NAD binding
A	0051402	biological_process	neuron apoptotic process
A	0051873	biological_process	killing by host of symbiont cells
A	0061844	biological_process	antimicrobial humoral immune response mediated by antimicrobial peptide
A	0070062	cellular_component	extracellular exosome
A	0071346	biological_process	cellular response to type II interferon
A	0097452	cellular_component	GAIT complex
A	0097718	molecular_function	disordered domain specific binding
A	1990904	cellular_component	ribonucleoprotein complex
B	0000226	biological_process	microtubule cytoskeleton organization
B	0001819	biological_process	positive regulation of cytokine production
B	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005811	cellular_component	lipid droplet
B	0005829	cellular_component	cytosol
B	0005856	cellular_component	cytoskeleton
B	0005886	cellular_component	plasma membrane
B	0006006	biological_process	glucose metabolic process
B	0006096	biological_process	glycolytic process
B	0006417	biological_process	regulation of translation
B	0006915	biological_process	apoptotic process
B	0008017	molecular_function	microtubule binding
B	0010951	biological_process	negative regulation of endopeptidase activity
B	0015630	cellular_component	microtubule cytoskeleton
B	0016020	cellular_component	membrane
B	0016241	biological_process	regulation of macroautophagy
B	0016491	molecular_function	oxidoreductase activity
B	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
B	0016740	molecular_function	transferase activity
B	0017148	biological_process	negative regulation of translation
B	0019828	molecular_function	aspartic-type endopeptidase inhibitor activity
B	0031640	biological_process	killing of cells of another organism
B	0031965	cellular_component	nuclear membrane
B	0031982	cellular_component	vesicle
B	0032481	biological_process	positive regulation of type I interferon production
B	0035605	molecular_function	peptidyl-cysteine S-nitrosylase activity
B	0035606	biological_process	peptidyl-cysteine S-trans-nitrosylation
B	0042802	molecular_function	identical protein binding
B	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
B	0043231	cellular_component	intracellular membrane-bounded organelle
B	0045087	biological_process	innate immune response
B	0048471	cellular_component	perinuclear region of cytoplasm
B	0050661	molecular_function	NADP binding
B	0050821	biological_process	protein stabilization
B	0050832	biological_process	defense response to fungus
B	0051287	molecular_function	NAD binding
B	0051402	biological_process	neuron apoptotic process
B	0051873	biological_process	killing by host of symbiont cells
B	0061844	biological_process	antimicrobial humoral immune response mediated by antimicrobial peptide
B	0070062	cellular_component	extracellular exosome
B	0071346	biological_process	cellular response to type II interferon
B	0097452	cellular_component	GAIT complex
B	0097718	molecular_function	disordered domain specific binding
B	1990904	cellular_component	ribonucleoprotein complex
C	0000226	biological_process	microtubule cytoskeleton organization
C	0001819	biological_process	positive regulation of cytokine production
C	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005811	cellular_component	lipid droplet
C	0005829	cellular_component	cytosol
C	0005856	cellular_component	cytoskeleton
C	0005886	cellular_component	plasma membrane
C	0006006	biological_process	glucose metabolic process
C	0006096	biological_process	glycolytic process
C	0006417	biological_process	regulation of translation
C	0006915	biological_process	apoptotic process
C	0008017	molecular_function	microtubule binding
C	0010951	biological_process	negative regulation of endopeptidase activity
C	0015630	cellular_component	microtubule cytoskeleton
C	0016020	cellular_component	membrane
C	0016241	biological_process	regulation of macroautophagy
C	0016491	molecular_function	oxidoreductase activity
C	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
C	0016740	molecular_function	transferase activity
C	0017148	biological_process	negative regulation of translation
C	0019828	molecular_function	aspartic-type endopeptidase inhibitor activity
C	0031640	biological_process	killing of cells of another organism
C	0031965	cellular_component	nuclear membrane
C	0031982	cellular_component	vesicle
C	0032481	biological_process	positive regulation of type I interferon production
C	0035605	molecular_function	peptidyl-cysteine S-nitrosylase activity
C	0035606	biological_process	peptidyl-cysteine S-trans-nitrosylation
C	0042802	molecular_function	identical protein binding
C	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
C	0043231	cellular_component	intracellular membrane-bounded organelle
C	0045087	biological_process	innate immune response
C	0048471	cellular_component	perinuclear region of cytoplasm
C	0050661	molecular_function	NADP binding
C	0050821	biological_process	protein stabilization
C	0050832	biological_process	defense response to fungus
C	0051287	molecular_function	NAD binding
C	0051402	biological_process	neuron apoptotic process
C	0051873	biological_process	killing by host of symbiont cells
C	0061844	biological_process	antimicrobial humoral immune response mediated by antimicrobial peptide
C	0070062	cellular_component	extracellular exosome
C	0071346	biological_process	cellular response to type II interferon
C	0097452	cellular_component	GAIT complex
C	0097718	molecular_function	disordered domain specific binding
C	1990904	cellular_component	ribonucleoprotein complex
D	0000226	biological_process	microtubule cytoskeleton organization
D	0001819	biological_process	positive regulation of cytokine production
D	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005811	cellular_component	lipid droplet
D	0005829	cellular_component	cytosol
D	0005856	cellular_component	cytoskeleton
D	0005886	cellular_component	plasma membrane
D	0006006	biological_process	glucose metabolic process
D	0006096	biological_process	glycolytic process
D	0006417	biological_process	regulation of translation
D	0006915	biological_process	apoptotic process
D	0008017	molecular_function	microtubule binding
D	0010951	biological_process	negative regulation of endopeptidase activity
D	0015630	cellular_component	microtubule cytoskeleton
D	0016020	cellular_component	membrane
D	0016241	biological_process	regulation of macroautophagy
D	0016491	molecular_function	oxidoreductase activity
D	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
D	0016740	molecular_function	transferase activity
D	0017148	biological_process	negative regulation of translation
D	0019828	molecular_function	aspartic-type endopeptidase inhibitor activity
D	0031640	biological_process	killing of cells of another organism
D	0031965	cellular_component	nuclear membrane
D	0031982	cellular_component	vesicle
D	0032481	biological_process	positive regulation of type I interferon production
D	0035605	molecular_function	peptidyl-cysteine S-nitrosylase activity
D	0035606	biological_process	peptidyl-cysteine S-trans-nitrosylation
D	0042802	molecular_function	identical protein binding
D	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
D	0043231	cellular_component	intracellular membrane-bounded organelle
D	0045087	biological_process	innate immune response
D	0048471	cellular_component	perinuclear region of cytoplasm
D	0050661	molecular_function	NADP binding
D	0050821	biological_process	protein stabilization
D	0050832	biological_process	defense response to fungus
D	0051287	molecular_function	NAD binding
D	0051402	biological_process	neuron apoptotic process
D	0051873	biological_process	killing by host of symbiont cells
D	0061844	biological_process	antimicrobial humoral immune response mediated by antimicrobial peptide
D	0070062	cellular_component	extracellular exosome
D	0071346	biological_process	cellular response to type II interferon
D	0097452	cellular_component	GAIT complex
D	0097718	molecular_function	disordered domain specific binding
D	1990904	cellular_component	ribonucleoprotein complex
E	0000226	biological_process	microtubule cytoskeleton organization
E	0001819	biological_process	positive regulation of cytokine production
E	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005811	cellular_component	lipid droplet
E	0005829	cellular_component	cytosol
E	0005856	cellular_component	cytoskeleton
E	0005886	cellular_component	plasma membrane
E	0006006	biological_process	glucose metabolic process
E	0006096	biological_process	glycolytic process
E	0006417	biological_process	regulation of translation
E	0006915	biological_process	apoptotic process
E	0008017	molecular_function	microtubule binding
E	0010951	biological_process	negative regulation of endopeptidase activity
E	0015630	cellular_component	microtubule cytoskeleton
E	0016020	cellular_component	membrane
E	0016241	biological_process	regulation of macroautophagy
E	0016491	molecular_function	oxidoreductase activity
E	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
E	0016740	molecular_function	transferase activity
E	0017148	biological_process	negative regulation of translation
E	0019828	molecular_function	aspartic-type endopeptidase inhibitor activity
E	0031640	biological_process	killing of cells of another organism
E	0031965	cellular_component	nuclear membrane
E	0031982	cellular_component	vesicle
E	0032481	biological_process	positive regulation of type I interferon production
E	0035605	molecular_function	peptidyl-cysteine S-nitrosylase activity
E	0035606	biological_process	peptidyl-cysteine S-trans-nitrosylation
E	0042802	molecular_function	identical protein binding
E	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
E	0043231	cellular_component	intracellular membrane-bounded organelle
E	0045087	biological_process	innate immune response
E	0048471	cellular_component	perinuclear region of cytoplasm
E	0050661	molecular_function	NADP binding
E	0050821	biological_process	protein stabilization
E	0050832	biological_process	defense response to fungus
E	0051287	molecular_function	NAD binding
E	0051402	biological_process	neuron apoptotic process
E	0051873	biological_process	killing by host of symbiont cells
E	0061844	biological_process	antimicrobial humoral immune response mediated by antimicrobial peptide
E	0070062	cellular_component	extracellular exosome
E	0071346	biological_process	cellular response to type II interferon
E	0097452	cellular_component	GAIT complex
E	0097718	molecular_function	disordered domain specific binding
E	1990904	cellular_component	ribonucleoprotein complex
F	0000226	biological_process	microtubule cytoskeleton organization
F	0001819	biological_process	positive regulation of cytokine production
F	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005737	cellular_component	cytoplasm
F	0005811	cellular_component	lipid droplet
F	0005829	cellular_component	cytosol
F	0005856	cellular_component	cytoskeleton
F	0005886	cellular_component	plasma membrane
F	0006006	biological_process	glucose metabolic process
F	0006096	biological_process	glycolytic process
F	0006417	biological_process	regulation of translation
F	0006915	biological_process	apoptotic process
F	0008017	molecular_function	microtubule binding
F	0010951	biological_process	negative regulation of endopeptidase activity
F	0015630	cellular_component	microtubule cytoskeleton
F	0016020	cellular_component	membrane
F	0016241	biological_process	regulation of macroautophagy
F	0016491	molecular_function	oxidoreductase activity
F	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
F	0016740	molecular_function	transferase activity
F	0017148	biological_process	negative regulation of translation
F	0019828	molecular_function	aspartic-type endopeptidase inhibitor activity
F	0031640	biological_process	killing of cells of another organism
F	0031965	cellular_component	nuclear membrane
F	0031982	cellular_component	vesicle
F	0032481	biological_process	positive regulation of type I interferon production
F	0035605	molecular_function	peptidyl-cysteine S-nitrosylase activity
F	0035606	biological_process	peptidyl-cysteine S-trans-nitrosylation
F	0042802	molecular_function	identical protein binding
F	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
F	0043231	cellular_component	intracellular membrane-bounded organelle
F	0045087	biological_process	innate immune response
F	0048471	cellular_component	perinuclear region of cytoplasm
F	0050661	molecular_function	NADP binding
F	0050821	biological_process	protein stabilization
F	0050832	biological_process	defense response to fungus
F	0051287	molecular_function	NAD binding
F	0051402	biological_process	neuron apoptotic process
F	0051873	biological_process	killing by host of symbiont cells
F	0061844	biological_process	antimicrobial humoral immune response mediated by antimicrobial peptide
F	0070062	cellular_component	extracellular exosome
F	0071346	biological_process	cellular response to type II interferon
F	0097452	cellular_component	GAIT complex
F	0097718	molecular_function	disordered domain specific binding
F	1990904	cellular_component	ribonucleoprotein complex
G	0000226	biological_process	microtubule cytoskeleton organization
G	0001819	biological_process	positive regulation of cytokine production
G	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005737	cellular_component	cytoplasm
G	0005811	cellular_component	lipid droplet
G	0005829	cellular_component	cytosol
G	0005856	cellular_component	cytoskeleton
G	0005886	cellular_component	plasma membrane
G	0006006	biological_process	glucose metabolic process
G	0006096	biological_process	glycolytic process
G	0006417	biological_process	regulation of translation
G	0006915	biological_process	apoptotic process
G	0008017	molecular_function	microtubule binding
G	0010951	biological_process	negative regulation of endopeptidase activity
G	0015630	cellular_component	microtubule cytoskeleton
G	0016020	cellular_component	membrane
G	0016241	biological_process	regulation of macroautophagy
G	0016491	molecular_function	oxidoreductase activity
G	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
G	0016740	molecular_function	transferase activity
G	0017148	biological_process	negative regulation of translation
G	0019828	molecular_function	aspartic-type endopeptidase inhibitor activity
G	0031640	biological_process	killing of cells of another organism
G	0031965	cellular_component	nuclear membrane
G	0031982	cellular_component	vesicle
G	0032481	biological_process	positive regulation of type I interferon production
G	0035605	molecular_function	peptidyl-cysteine S-nitrosylase activity
G	0035606	biological_process	peptidyl-cysteine S-trans-nitrosylation
G	0042802	molecular_function	identical protein binding
G	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
G	0043231	cellular_component	intracellular membrane-bounded organelle
G	0045087	biological_process	innate immune response
G	0048471	cellular_component	perinuclear region of cytoplasm
G	0050661	molecular_function	NADP binding
G	0050821	biological_process	protein stabilization
G	0050832	biological_process	defense response to fungus
G	0051287	molecular_function	NAD binding
G	0051402	biological_process	neuron apoptotic process
G	0051873	biological_process	killing by host of symbiont cells
G	0061844	biological_process	antimicrobial humoral immune response mediated by antimicrobial peptide
G	0070062	cellular_component	extracellular exosome
G	0071346	biological_process	cellular response to type II interferon
G	0097452	cellular_component	GAIT complex
G	0097718	molecular_function	disordered domain specific binding
G	1990904	cellular_component	ribonucleoprotein complex
O	0000226	biological_process	microtubule cytoskeleton organization
O	0001819	biological_process	positive regulation of cytokine production
O	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
O	0005515	molecular_function	protein binding
O	0005634	cellular_component	nucleus
O	0005737	cellular_component	cytoplasm
O	0005811	cellular_component	lipid droplet
O	0005829	cellular_component	cytosol
O	0005856	cellular_component	cytoskeleton
O	0005886	cellular_component	plasma membrane
O	0006006	biological_process	glucose metabolic process
O	0006096	biological_process	glycolytic process
O	0006417	biological_process	regulation of translation
O	0006915	biological_process	apoptotic process
O	0008017	molecular_function	microtubule binding
O	0010951	biological_process	negative regulation of endopeptidase activity
O	0015630	cellular_component	microtubule cytoskeleton
O	0016020	cellular_component	membrane
O	0016241	biological_process	regulation of macroautophagy
O	0016491	molecular_function	oxidoreductase activity
O	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
O	0016740	molecular_function	transferase activity
O	0017148	biological_process	negative regulation of translation
O	0019828	molecular_function	aspartic-type endopeptidase inhibitor activity
O	0031640	biological_process	killing of cells of another organism
O	0031965	cellular_component	nuclear membrane
O	0031982	cellular_component	vesicle
O	0032481	biological_process	positive regulation of type I interferon production
O	0035605	molecular_function	peptidyl-cysteine S-nitrosylase activity
O	0035606	biological_process	peptidyl-cysteine S-trans-nitrosylation
O	0042802	molecular_function	identical protein binding
O	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
O	0043231	cellular_component	intracellular membrane-bounded organelle
O	0045087	biological_process	innate immune response
O	0048471	cellular_component	perinuclear region of cytoplasm
O	0050661	molecular_function	NADP binding
O	0050821	biological_process	protein stabilization
O	0050832	biological_process	defense response to fungus
O	0051287	molecular_function	NAD binding
O	0051402	biological_process	neuron apoptotic process
O	0051873	biological_process	killing by host of symbiont cells
O	0061844	biological_process	antimicrobial humoral immune response mediated by antimicrobial peptide
O	0070062	cellular_component	extracellular exosome
O	0071346	biological_process	cellular response to type II interferon
O	0097452	cellular_component	GAIT complex
O	0097718	molecular_function	disordered domain specific binding
O	1990904	cellular_component	ribonucleoprotein complex

Functional Information from PDB Data

site_id	AC1
Number of Residues	31
Details	binding site for residue NAD O 401

Chain	Residue
O	ASN9
O	PHE37
O	ARG80
O	SER98
O	THR99
O	GLY100
O	SER122
O	ALA123
O	CYS152
O	ALA183
O	ASN316
O	GLY10
O	TYR320
O	HOH535
O	HOH544
O	HOH561
O	HOH566
O	HOH620
O	HOH644
O	HOH664
O	HOH716
O	HOH718
O	PHE11
O	HOH788
O	HOH816
O	GLY12
O	ARG13
O	ILE14
O	ASN34
O	ASP35
O	PRO36

---

site_id	AC2
Number of Residues	4
Details	binding site for residue ZN O 402

Chain	Residue
E	GLU106
E	ASP127
O	HIS306
O	ACT403

---

site_id	AC3
Number of Residues	8
Details	binding site for residue ACT O 403

Chain	Residue
E	THR104
E	GLU106
E	ALA126
E	ASP127
O	HIS306
O	ZN402
O	HOH819
O	HOH820

---

site_id	AC4
Number of Residues	32
Details	binding site for residue NAD A 401

Chain	Residue
A	ASN9
A	GLY10
A	PHE11
A	GLY12
A	ARG13
A	ILE14
A	ASN34
A	ASP35
A	PRO36
A	PHE37
A	ARG80
A	SER98
A	THR99
A	GLY100
A	SER122
A	ALA123
A	CYS152
A	ALA183
A	ASN316
A	TYR320
A	HOH551
A	HOH556
A	HOH571
A	HOH572
A	HOH643
A	HOH694
A	HOH709
A	HOH813
A	HOH815
A	HOH849
A	HOH851
A	HOH852

---

site_id	AC5
Number of Residues	1
Details	binding site for residue ZN A 402

Chain	Residue
A	GLU63

---

site_id	AC6
Number of Residues	31
Details	binding site for residue NAD B 401

Chain	Residue
B	HOH577
B	HOH628
B	HOH671
B	HOH685
B	HOH803
B	HOH833
B	HOH834
B	HOH836
B	ASN9
B	GLY10
B	PHE11
B	GLY12
B	ARG13
B	ILE14
B	ASN34
B	ASP35
B	PRO36
B	PHE37
B	ARG80
B	SER98
B	THR99
B	GLY100
B	SER122
B	ALA123
B	CYS152
B	ALA183
B	ASN316
B	TYR320
B	HOH552
B	HOH553
B	HOH567

---

site_id	AC7
Number of Residues	1
Details	binding site for residue ZN B 402

Chain	Residue
B	GLU63

---

site_id	AC8
Number of Residues	28
Details	binding site for residue NAD C 401

Chain	Residue
C	ASN9
C	GLY10
C	PHE11
C	GLY12
C	ARG13
C	ILE14
C	ASP35
C	PRO36
C	PHE37
C	ARG80
C	SER98
C	THR99
C	GLY100
C	PHE102
C	SER122
C	ALA123
C	CYS152
C	ALA183
C	ASN316
C	TYR320
C	HOH533
C	HOH534
C	HOH561
C	HOH590
C	HOH642
C	HOH726
C	HOH763
C	HOH765

---

site_id	AC9
Number of Residues	3
Details	binding site for residue ZN C 402

Chain	Residue
C	GLU335
C	HOH501
C	HOH509

---

site_id	AD1
Number of Residues	4
Details	binding site for residue ZN C 403

Chain	Residue
C	GLU106
C	ASP127
C	ACT404
D	HIS306

---

site_id	AD2
Number of Residues	7
Details	binding site for residue ACT C 404

Chain	Residue
C	THR104
C	GLU106
C	ASP127
C	ZN403
C	HOH502
C	HOH503
D	HIS306

---

site_id	AD3
Number of Residues	30
Details	binding site for residue NAD D 401

Chain	Residue
D	ASN9
D	GLY10
D	PHE11
D	GLY12
D	ARG13
D	ILE14
D	ASN34
D	ASP35
D	PRO36
D	PHE37
D	ARG80
D	SER98
D	THR99
D	GLY100
D	SER122
D	ALA123
D	CYS152
D	ALA183
D	ASN316
D	TYR320
D	HOH535
D	HOH541
D	HOH567
D	HOH581
D	HOH637
D	HOH676
D	HOH681
D	HOH709
D	HOH818
D	HOH819

---

site_id	AD4
Number of Residues	29
Details	binding site for residue NAD E 401

Chain	Residue
E	ASN9
E	GLY10
E	PHE11
E	GLY12
E	ARG13
E	ILE14
E	ASP35
E	PRO36
E	PHE37
E	ARG80
E	SER98
E	THR99
E	GLY100
E	PHE102
E	SER122
E	CYS152
E	ALA183
E	ASN316
E	TYR320
E	HOH523
E	HOH537
E	HOH552
E	HOH579
E	HOH644
E	HOH657
E	HOH689
E	HOH702
E	HOH712
E	HOH724

---

site_id	AD5
Number of Residues	3
Details	binding site for residue ZN E 402

Chain	Residue
E	GLU335
E	HOH505
E	HOH510

---

Functional Information from PROSITE/UniProt

site_id	PS00071
Number of Residues	8
Details	GAPDH Glyceraldehyde 3-phosphate dehydrogenase active site. ASCTTNcL

Chain	Residue	Details
O	ALA150-LEU157

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: Nucleophile => ECO:0000269|PubMed:25086035

Chain	Residue	Details
O	CYS152
A	CYS152
B	CYS152
C	CYS152
D	CYS152
E	CYS152
F	CYS152
G	CYS152

---

site_id	SWS_FT_FI2
Number of Residues	40
Details	BINDING: BINDING => ECO:0000269|PubMed:16239728, ECO:0000269|PubMed:16510976

Chain	Residue	Details
O	ARG13
A	ASN316
B	ARG13
B	ASP35
B	ARG80
B	SER122
B	ASN316
C	ARG13
C	ASP35
C	ARG80
C	SER122
O	ASP35
C	ASN316
D	ARG13
D	ASP35
D	ARG80
D	SER122
D	ASN316
E	ARG13
E	ASP35
E	ARG80
E	SER122
O	ARG80
E	ASN316
F	ARG13
F	ASP35
F	ARG80
F	SER122
F	ASN316
G	ARG13
G	ASP35
G	ARG80
G	SER122
O	SER122
G	ASN316
O	ASN316
A	ARG13
A	ASP35
A	ARG80
A	SER122

---

site_id	SWS_FT_FI3
Number of Residues	32
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P22513

Chain	Residue	Details
O	SER151
B	THR182
B	THR211
B	ARG234
C	SER151
C	THR182
C	THR211
C	ARG234
D	SER151
D	THR182
D	THR211
O	THR182
D	ARG234
E	SER151
E	THR182
E	THR211
E	ARG234
F	SER151
F	THR182
F	THR211
F	ARG234
G	SER151
O	THR211
G	THR182
G	THR211
G	ARG234
O	ARG234
A	SER151
A	THR182
A	THR211
A	ARG234
B	SER151

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	SITE: Activates thiol group during catalysis => ECO:0000305|PubMed:16239728, ECO:0000305|PubMed:16510976

Chain	Residue	Details
O	HIS179
A	HIS179
B	HIS179
C	HIS179
D	HIS179
E	HIS179
F	HIS179
G	HIS179

---

site_id	SWS_FT_FI5
Number of Residues	40
Details	MOD_RES: N6,N6-dimethyllysine => ECO:0000269|PubMed:18183946

Chain	Residue	Details
O	LYS5
A	LYS334
B	LYS5
B	LYS66
B	LYS260
B	LYS263
B	LYS334
C	LYS5
C	LYS66
C	LYS260
C	LYS263
O	LYS66
C	LYS334
D	LYS5
D	LYS66
D	LYS260
D	LYS263
D	LYS334
E	LYS5
E	LYS66
E	LYS260
E	LYS263
O	LYS260
E	LYS334
F	LYS5
F	LYS66
F	LYS260
F	LYS263
F	LYS334
G	LYS5
G	LYS66
G	LYS260
G	LYS263
O	LYS263
G	LYS334
O	LYS334
A	LYS5
A	LYS66
A	LYS260
A	LYS263

---

site_id	SWS_FT_FI6
Number of Residues	56
Details	MOD_RES: Deamidated asparagine => ECO:0000269|PubMed:18183946

Chain	Residue	Details
O	ASN9
A	ASN70
A	ASN149
A	ASN155
A	ASN225
A	ASN316
B	ASN9
B	ASN64
B	ASN70
B	ASN149
B	ASN155
O	ASN64
B	ASN225
B	ASN316
C	ASN9
C	ASN64
C	ASN70
C	ASN149
C	ASN155
C	ASN225
C	ASN316
D	ASN9
O	ASN70
D	ASN64
D	ASN70
D	ASN149
D	ASN155
D	ASN225
D	ASN316
E	ASN9
E	ASN64
E	ASN70
E	ASN149
O	ASN149
E	ASN155
E	ASN225
E	ASN316
F	ASN9
F	ASN64
F	ASN70
F	ASN149
F	ASN155
F	ASN225
F	ASN316
O	ASN155
G	ASN9
G	ASN64
G	ASN70
G	ASN149
G	ASN155
G	ASN225
G	ASN316
O	ASN225
O	ASN316
A	ASN9
A	ASN64

---

site_id	SWS_FT_FI7
Number of Residues	8
Details	MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:15592455

Chain	Residue	Details
O	TYR42
A	TYR42
B	TYR42
C	TYR42
D	TYR42
E	TYR42
F	TYR42
G	TYR42

---

site_id	SWS_FT_FI8
Number of Residues	8
Details	MOD_RES: Methionine sulfoxide; in vitro => ECO:0000305|PubMed:25086035

Chain	Residue	Details
O	MET46
A	MET46
B	MET46
C	MET46
D	MET46
E	MET46
F	MET46
G	MET46

---

site_id	SWS_FT_FI9
Number of Residues	24
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
O	LYS61
C	LYS61
C	LYS219
C	LYS254
D	LYS61
D	LYS219
D	LYS254
E	LYS61
E	LYS219
E	LYS254
F	LYS61
O	LYS219
F	LYS219
F	LYS254
G	LYS61
G	LYS219
G	LYS254
O	LYS254
A	LYS61
A	LYS219
A	LYS254
B	LYS61
B	LYS219
B	LYS254

---

site_id	SWS_FT_FI10
Number of Residues	8
Details	MOD_RES: Phosphothreonine => ECO:0000269|PubMed:18183946, ECO:0007744|PubMed:20068231

Chain	Residue	Details
O	THR75
A	THR75
B	THR75
C	THR75
D	THR75
E	THR75
F	THR75
G	THR75

---

site_id	SWS_FT_FI11
Number of Residues	8
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:17081983, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163

Chain	Residue	Details
O	SER83
A	SER83
B	SER83
C	SER83
D	SER83
E	SER83
F	SER83
G	SER83

---

site_id	SWS_FT_FI12
Number of Residues	16
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:18183946

Chain	Residue	Details
O	SER122
D	SER148
E	SER122
E	SER148
F	SER122
F	SER148
G	SER122
G	SER148
O	SER148
A	SER122
A	SER148
B	SER122
B	SER148
C	SER122
C	SER148
D	SER122

---

site_id	SWS_FT_FI13
Number of Residues	8
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:23186163

Chain	Residue	Details
O	SER151
A	SER151
B	SER151
C	SER151
D	SER151
E	SER151
F	SER151
G	SER151

---

site_id	SWS_FT_FI14
Number of Residues	8
Details	MOD_RES: S-nitrosocysteine; in reversibly inhibited form => ECO:0000250|UniProtKB:P04797

Chain	Residue	Details
O	CYS152
A	CYS152
B	CYS152
C	CYS152
D	CYS152
E	CYS152
F	CYS152
G	CYS152

---

site_id	SWS_FT_FI15
Number of Residues	8
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
O	THR153
A	THR153
B	THR153
C	THR153
D	THR153
E	THR153
F	THR153
G	THR153

---

site_id	SWS_FT_FI16
Number of Residues	16
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:24275569

Chain	Residue	Details
O	THR177
D	THR182
E	THR177
E	THR182
F	THR177
F	THR182
G	THR177
G	THR182
O	THR182
A	THR177
A	THR182
B	THR177
B	THR182
C	THR177
C	THR182
D	THR177

---

site_id	SWS_FT_FI17
Number of Residues	8
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:24275569

Chain	Residue	Details
O	THR184
A	THR184
B	THR184
C	THR184
D	THR184
E	THR184
F	THR184
G	THR184

---

site_id	SWS_FT_FI18
Number of Residues	16
Details	MOD_RES: N6-malonyllysine; alternate => ECO:0000269|PubMed:21908771

Chain	Residue	Details
O	LYS194
D	LYS215
E	LYS194
E	LYS215
F	LYS194
F	LYS215
G	LYS194
G	LYS215
O	LYS215
A	LYS194
A	LYS215
B	LYS194
B	LYS215
C	LYS194
C	LYS215
D	LYS194

---

site_id	SWS_FT_FI19
Number of Residues	8
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:19690332

Chain	Residue	Details
O	THR211
A	THR211
B	THR211
C	THR211
D	THR211
E	THR211
F	THR211
G	THR211

---

site_id	SWS_FT_FI20
Number of Residues	8
Details	MOD_RES: N6-acetyllysine; alternate => ECO:0007744|PubMed:19608861

Chain	Residue	Details
O	LYS227
A	LYS227
B	LYS227
C	LYS227
D	LYS227
E	LYS227
F	LYS227
G	LYS227

---

site_id	SWS_FT_FI21
Number of Residues	8
Details	MOD_RES: Phosphothreonine => ECO:0000269|PubMed:18183946, ECO:0007744|PubMed:23186163

Chain	Residue	Details
O	THR229
A	THR229
B	THR229
C	THR229
D	THR229
E	THR229
F	THR229
G	THR229

---

site_id	SWS_FT_FI22
Number of Residues	8
Details	MOD_RES: Phosphothreonine => ECO:0000269|PubMed:18183946

Chain	Residue	Details
O	THR237
A	THR237
B	THR237
C	THR237
D	THR237
E	THR237
F	THR237
G	THR237

---

site_id	SWS_FT_FI23
Number of Residues	8
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:24275569

Chain	Residue	Details
O	SER241
A	SER241
B	SER241
C	SER241
D	SER241
E	SER241
F	SER241
G	SER241

---

site_id	SWS_FT_FI24
Number of Residues	8
Details	MOD_RES: S-nitrosocysteine => ECO:0000269|PubMed:22771119, ECO:0000269|PubMed:25417112

Chain	Residue	Details
O	CYS247
A	CYS247
B	CYS247
C	CYS247
D	CYS247
E	CYS247
F	CYS247
G	CYS247

---

site_id	SWS_FT_FI25
Number of Residues	8
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:18183946, ECO:0007744|PubMed:19690332

Chain	Residue	Details
O	SER312
A	SER312
B	SER312
C	SER312
D	SER312
E	SER312
F	SER312
G	SER312

---

site_id	SWS_FT_FI26
Number of Residues	8
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
O	SER333
A	SER333
B	SER333
C	SER333
D	SER333
E	SER333
F	SER333
G	SER333

---

site_id	SWS_FT_FI27
Number of Residues	16
Details	CARBOHYD: (Microbial infection) N-beta-linked (GlcNAc) arginine => ECO:0000269|PubMed:28522607

Chain	Residue	Details
O	ARG197
D	ARG200
E	ARG197
E	ARG200
F	ARG197
F	ARG200
G	ARG197
G	ARG200
O	ARG200
A	ARG197
A	ARG200
B	ARG197
B	ARG200
C	ARG197
C	ARG200
D	ARG197

---

site_id	SWS_FT_FI28
Number of Residues	16
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733

Chain	Residue	Details
O	LYS186
E	LYS186
F	LYS186
G	LYS186
A	LYS186
B	LYS186
C	LYS186
D	LYS186

---