Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4UP2

Crystal structure of Escherichia coli tryptophanase purified from alkaline stressed bacterial culture.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006520	biological_process	amino acid metabolic process
A	0006568	biological_process	L-tryptophan metabolic process
A	0006569	biological_process	L-tryptophan catabolic process
A	0009034	molecular_function	tryptophanase activity
A	0009072	biological_process	aromatic amino acid metabolic process
A	0016020	cellular_component	membrane
A	0016829	molecular_function	lyase activity
A	0016830	molecular_function	carbon-carbon lyase activity
A	0030170	molecular_function	pyridoxal phosphate binding
A	0030955	molecular_function	potassium ion binding
A	0032991	cellular_component	protein-containing complex
A	0042431	biological_process	indole metabolic process
A	0042802	molecular_function	identical protein binding
A	0060187	cellular_component	cell pole
A	0080146	molecular_function	L-cysteine desulfhydrase activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006520	biological_process	amino acid metabolic process
B	0006568	biological_process	L-tryptophan metabolic process
B	0006569	biological_process	L-tryptophan catabolic process
B	0009034	molecular_function	tryptophanase activity
B	0009072	biological_process	aromatic amino acid metabolic process
B	0016020	cellular_component	membrane
B	0016829	molecular_function	lyase activity
B	0016830	molecular_function	carbon-carbon lyase activity
B	0030170	molecular_function	pyridoxal phosphate binding
B	0030955	molecular_function	potassium ion binding
B	0032991	cellular_component	protein-containing complex
B	0042431	biological_process	indole metabolic process
B	0042802	molecular_function	identical protein binding
B	0060187	cellular_component	cell pole
B	0080146	molecular_function	L-cysteine desulfhydrase activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006520	biological_process	amino acid metabolic process
C	0006568	biological_process	L-tryptophan metabolic process
C	0006569	biological_process	L-tryptophan catabolic process
C	0009034	molecular_function	tryptophanase activity
C	0009072	biological_process	aromatic amino acid metabolic process
C	0016020	cellular_component	membrane
C	0016829	molecular_function	lyase activity
C	0016830	molecular_function	carbon-carbon lyase activity
C	0030170	molecular_function	pyridoxal phosphate binding
C	0030955	molecular_function	potassium ion binding
C	0032991	cellular_component	protein-containing complex
C	0042431	biological_process	indole metabolic process
C	0042802	molecular_function	identical protein binding
C	0060187	cellular_component	cell pole
C	0080146	molecular_function	L-cysteine desulfhydrase activity
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006520	biological_process	amino acid metabolic process
D	0006568	biological_process	L-tryptophan metabolic process
D	0006569	biological_process	L-tryptophan catabolic process
D	0009034	molecular_function	tryptophanase activity
D	0009072	biological_process	aromatic amino acid metabolic process
D	0016020	cellular_component	membrane
D	0016829	molecular_function	lyase activity
D	0016830	molecular_function	carbon-carbon lyase activity
D	0030170	molecular_function	pyridoxal phosphate binding
D	0030955	molecular_function	potassium ion binding
D	0032991	cellular_component	protein-containing complex
D	0042431	biological_process	indole metabolic process
D	0042802	molecular_function	identical protein binding
D	0060187	cellular_component	cell pole
D	0080146	molecular_function	L-cysteine desulfhydrase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 A 1469

Chain	Residue
A	SER54
A	GLN101
A	GLY102
A	ARG103
A	SER267
A	LYS269

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 A 1470

Chain	Residue
B	GLY55
B	PRO275
A	GLU72
A	LEU310
B	SER54

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 A 1471

Chain	Residue
A	VAL388
A	ALA389
A	PHE434
A	GLU437
A	ALA438

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 C 1455

Chain	Residue
A	MET1
C	VAL388
C	ALA389
C	PHE434
C	GLU437
C	ALA438

site_id	AC5
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 A 1472

Chain	Residue
A	THR23
A	ARG24

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 1473

Chain	Residue
A	ALA84
A	GLN94
A	HOH2018
A	HOH2039

site_id	AC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 1474

Chain	Residue
A	THR52
A	ASP53
A	LYS270
A	ARG419

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 B 1454

Chain	Residue
A	GLY55
A	PRO275
B	GLU72
B	LEU310

site_id	AC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 1455

Chain	Residue
B	TRP248
B	GLN252
B	HOH2029

site_id	BC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 B 1456

Chain	Residue
B	TYR74
B	TYR307

site_id	BC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 1457

Chain	Residue
B	TYR307
B	HOH2030
B	HOH2031

site_id	BC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 B 1458

Chain	Residue
B	GLY102
B	ARG103
B	SER267
B	LYS269

site_id	BC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 D 1455

Chain	Residue
B	GLU2
D	VAL388
D	ALA389
D	PHE434
D	GLU437
D	ALA438

site_id	BC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 B 1459

Chain	Residue
B	THR60
B	SER62
B	ASP324
B	HOH2023
D	PRO18

site_id	BC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 B 1460

Chain	Residue
B	THR22
B	THR23
B	ARG24
B	ARG27

site_id	BC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE BME B 1461

Chain	Residue
B	SER75
B	GLY76

site_id	BC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 C 1456

Chain	Residue
C	GLN101
C	GLY102
C	ARG103
C	SER267
C	LYS269

site_id	BC9
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 C 1457

Chain	Residue
C	THR52
C	LYS270

site_id	CC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 C 1458

Chain	Residue
C	THR22
C	THR23
C	ARG24
C	ARG27
C	HOH2004
C	HOH2024

site_id	CC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 D 1456

Chain	Residue
C	GLU72
D	SER54
D	GLY55
D	LYS269
D	PRO275

site_id	CC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 C 1459

Chain	Residue
C	GLN94
C	HOH2011

site_id	CC4
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 C 1460

Chain	Residue
C	SER398

site_id	CC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 C 1461

Chain	Residue
B	LYS211
C	LYS211
C	GLU244
C	TYR245
C	GLU256

site_id	CC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 C 1462

Chain	Residue
C	PRO414

site_id	CC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 D 1457

Chain	Residue
C	SER54
C	GLY55
D	GLU72
D	ALA73

site_id	CC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 D 1458

Chain	Residue
D	SER267
D	LYS269
D	GLN101
D	GLY102
D	ARG103

site_id	CC9
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 D 1459

Chain	Residue
C	GLN101
D	LEU310

site_id	DC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 D 1460

Chain	Residue
D	LEU452
D	THR453

site_id	DC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 B 1462

Chain	Residue
B	VAL388
B	PHE434
B	GLU437
B	ALA438
D	GLU2

Functional Information from PROSITE/UniProt

site_id	PS00853
Number of Residues	19
Details	BETA_ELIM_LYASE Beta-eliminating lyases pyridoxal-phosphate attachment site. YaDmlaMSAKKDaMVpMGG

Chain	Residue	Details
A	TYR260-GLY278

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)