4UP2
Crystal structure of Escherichia coli tryptophanase purified from alkaline stressed bacterial culture.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-25 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 158.240, 158.240, 387.766 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.014 - 2.780 |
| R-factor | 0.217 |
| Rwork | 0.215 |
| R-free | 0.24570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c44 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.696 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.010 | 2.880 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Rmerge | 0.175 | 0.210 |
| Number of reflections | 72775 | |
| <I/σ(I)> | 25.72 | 2.54 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 42.6 | 43.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25% PEG 4000 30MM AMMONIUM SULFATE 20MM B-MERCAPTOETHANOL 100MM TRIS-HCL PH 9 |
