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SummaryStructural detailsExperimental detailsFunctional detailsSequence NeighborHistoryDownloads

4UP1

Crystal structure of native human Thymidylate synthase in active form

Functional Information from GO Data
ChainGOidnamespacecontents
A0000900molecular_functionmRNA regulatory element binding translation repressor activity
A0004799molecular_functionthymidylate synthase activity
A0005515molecular_functionprotein binding
A0005542molecular_functionfolic acid binding
A0005634cellular_componentnucleus
A0005657cellular_componentreplication fork
A0005737cellular_componentcytoplasm
A0005739cellular_componentmitochondrion
A0005743cellular_componentmitochondrial inner membrane
A0005759cellular_componentmitochondrial matrix
A0005829cellular_componentcytosol
A0006207biological_process'de novo' pyrimidine nucleobase biosynthetic process
A0006231biological_processdTMP biosynthetic process
A0006235biological_processdTTP biosynthetic process
A0006260biological_processDNA replication
A0008168molecular_functionmethyltransferase activity
A0009165biological_processnucleotide biosynthetic process
A0016740molecular_functiontransferase activity
A0016741molecular_functiontransferase activity, transferring one-carbon groups
A0017148biological_processnegative regulation of translation
A0032259biological_processmethylation
A0035999biological_processtetrahydrofolate interconversion
A0046653biological_processtetrahydrofolate metabolic process
A0071897biological_processDNA biosynthetic process
A1990825molecular_functionsequence-specific mRNA binding
B0000900molecular_functionmRNA regulatory element binding translation repressor activity
B0004799molecular_functionthymidylate synthase activity
B0005515molecular_functionprotein binding
B0005542molecular_functionfolic acid binding
B0005634cellular_componentnucleus
B0005657cellular_componentreplication fork
B0005737cellular_componentcytoplasm
B0005739cellular_componentmitochondrion
B0005743cellular_componentmitochondrial inner membrane
B0005759cellular_componentmitochondrial matrix
B0005829cellular_componentcytosol
B0006207biological_process'de novo' pyrimidine nucleobase biosynthetic process
B0006231biological_processdTMP biosynthetic process
B0006235biological_processdTTP biosynthetic process
B0006260biological_processDNA replication
B0008168molecular_functionmethyltransferase activity
B0009165biological_processnucleotide biosynthetic process
B0016740molecular_functiontransferase activity
B0016741molecular_functiontransferase activity, transferring one-carbon groups
B0017148biological_processnegative regulation of translation
B0032259biological_processmethylation
B0035999biological_processtetrahydrofolate interconversion
B0046653biological_processtetrahydrofolate metabolic process
B0071897biological_processDNA biosynthetic process
B1990825molecular_functionsequence-specific mRNA binding
C0000900molecular_functionmRNA regulatory element binding translation repressor activity
C0004799molecular_functionthymidylate synthase activity
C0005515molecular_functionprotein binding
C0005542molecular_functionfolic acid binding
C0005634cellular_componentnucleus
C0005657cellular_componentreplication fork
C0005737cellular_componentcytoplasm
C0005739cellular_componentmitochondrion
C0005743cellular_componentmitochondrial inner membrane
C0005759cellular_componentmitochondrial matrix
C0005829cellular_componentcytosol
C0006207biological_process'de novo' pyrimidine nucleobase biosynthetic process
C0006231biological_processdTMP biosynthetic process
C0006235biological_processdTTP biosynthetic process
C0006260biological_processDNA replication
C0008168molecular_functionmethyltransferase activity
C0009165biological_processnucleotide biosynthetic process
C0016740molecular_functiontransferase activity
C0016741molecular_functiontransferase activity, transferring one-carbon groups
C0017148biological_processnegative regulation of translation
C0032259biological_processmethylation
C0035999biological_processtetrahydrofolate interconversion
C0046653biological_processtetrahydrofolate metabolic process
C0071897biological_processDNA biosynthetic process
C1990825molecular_functionsequence-specific mRNA binding
D0000900molecular_functionmRNA regulatory element binding translation repressor activity
D0004799molecular_functionthymidylate synthase activity
D0005515molecular_functionprotein binding
D0005542molecular_functionfolic acid binding
D0005634cellular_componentnucleus
D0005657cellular_componentreplication fork
D0005737cellular_componentcytoplasm
D0005739cellular_componentmitochondrion
D0005743cellular_componentmitochondrial inner membrane
D0005759cellular_componentmitochondrial matrix
D0005829cellular_componentcytosol
D0006207biological_process'de novo' pyrimidine nucleobase biosynthetic process
D0006231biological_processdTMP biosynthetic process
D0006235biological_processdTTP biosynthetic process
D0006260biological_processDNA replication
D0008168molecular_functionmethyltransferase activity
D0009165biological_processnucleotide biosynthetic process
D0016740molecular_functiontransferase activity
D0016741molecular_functiontransferase activity, transferring one-carbon groups
D0017148biological_processnegative regulation of translation
D0032259biological_processmethylation
D0035999biological_processtetrahydrofolate interconversion
D0046653biological_processtetrahydrofolate metabolic process
D0071897biological_processDNA biosynthetic process
D1990825molecular_functionsequence-specific mRNA binding
Functional Information from PDB Data
site_idAC1
Number of Residues5
DetailsBINDING SITE FOR RESIDUE SO4 A 401
ChainResidue
AARG50
ALEU192
AARG215
BARG175
BARG176
site_idAC2
Number of Residues10
DetailsBINDING SITE FOR RESIDUE SO4 A 402
ChainResidue
AALA181
AHIS196
AALA197
ALEU198
ACYS199
ATYR230
ATYR135
ATRP139
AMET179
ACYS180
site_idAC3
Number of Residues4
DetailsBINDING SITE FOR RESIDUE SO4 A 403
ChainResidue
AARG42
ACYS43
AGLY44
AVAL45
site_idAC4
Number of Residues1
DetailsBINDING SITE FOR RESIDUE SO4 A 404
ChainResidue
APHE225
site_idAC5
Number of Residues4
DetailsBINDING SITE FOR RESIDUE SO4 B 401
ChainResidue
AARG175
AARG176
BARG50
BARG215
site_idAC6
Number of Residues6
DetailsBINDING SITE FOR RESIDUE SO4 B 402
ChainResidue
BARG46
BGLY52
BTHR53
BGLY54
DLEU41
DLYS266
site_idAC7
Number of Residues3
DetailsBINDING SITE FOR RESIDUE SO4 B 403
ChainResidue
BARG46
BLYS47
BASP48
site_idAC8
Number of Residues9
DetailsBINDING SITE FOR RESIDUE SO4 B 404
ChainResidue
BTYR135
BTRP139
BMET179
BALA181
BHIS196
BALA197
BLEU198
BCYS199
BTYR230
site_idAC9
Number of Residues4
DetailsBINDING SITE FOR RESIDUE SO4 B 405
ChainResidue
BLYS77
BGLY220
BHIS261
BPRO264
site_idBC1
Number of Residues4
DetailsBINDING SITE FOR RESIDUE SO4 C 401
ChainResidue
CARG175
CARG176
DARG50
DARG215
site_idBC2
Number of Residues4
DetailsBINDING SITE FOR RESIDUE SO4 C 402
ChainResidue
CASP173
DARG46
DLYS47
DASP48
site_idBC3
Number of Residues4
DetailsBINDING SITE FOR RESIDUE SO4 D 401
ChainResidue
CARG50
CARG215
DARG175
DARG176
Functional Information from PROSITE/UniProt
site_idPS00091
Number of Residues29
DetailsTHYMIDYLATE_SYNTHASE Thymidylate synthase active site. RriImcaWNprdlplma.....LpPCHalcQFyV
ChainResidueDetails
AARG175-VAL203
Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues4
DetailsActive site: {"description":"Nucleophile","evidences":[{"source":"UniProtKB","id":"P0A884","evidenceCode":"ECO:0000250"}]}
ChainResidueDetails
site_idSWS_FT_FI2
Number of Residues8
DetailsBinding site: {"description":"in other chain","evidences":[{"source":"UniProtKB","id":"P0A884","evidenceCode":"ECO:0000250"}]}
ChainResidueDetails
site_idSWS_FT_FI3
Number of Residues4
DetailsBinding site: {"evidences":[{"source":"UniProtKB","id":"P45352","evidenceCode":"ECO:0000250"}]}
ChainResidueDetails
site_idSWS_FT_FI4
Number of Residues24
DetailsBinding site: {"description":"in other chain","evidences":[{"source":"UniProtKB","id":"P45352","evidenceCode":"ECO:0000250"}]}
ChainResidueDetails
site_idSWS_FT_FI5
Number of Residues4
DetailsBinding site: {"evidences":[{"source":"UniProtKB","id":"P0A884","evidenceCode":"ECO:0000250"}]}
ChainResidueDetails
site_idSWS_FT_FI6
Number of Residues4
DetailsModified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"19690332","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}
ChainResidueDetails
site_idSWS_FT_FI7
Number of Residues15
DetailsCross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}
ChainResidueDetails

250359

PDB entries from 2026-03-11

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