4R8N

Crystal structure of Staphylococcal nuclease variant V23I/V66I/I72V/I92V at cryogenic temperature

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003676	molecular_function	nucleic acid binding
A	0004518	molecular_function	nuclease activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	18
Details	BINDING SITE FOR RESIDUE THP A 201

Chain	Residue
A	ARG35
A	TYR115
A	CA202
A	HOH304
A	HOH309
A	HOH315
A	HOH347
A	HOH362
A	HOH363
A	HOH372
A	ASP40
A	LYS71
A	ASP83
A	LYS84
A	TYR85
A	ARG87
A	LEU89
A	TYR113

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site_id	AC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA A 202

Chain	Residue
A	ASP21
A	ASP40
A	THR41
A	THP201
A	HOH339
A	HOH346
A	HOH347

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site_id	AC3
Number of Residues	1
Details	BINDING SITE FOR RESIDUE PO4 A 203

Chain	Residue
A	LYS134

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site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MRD A 204

Chain	Residue
A	PHE61
A	LYS64
A	LYS116
A	HOH343

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Functional Information from PROSITE/UniProt

site_id	PS01123
Number of Residues	25
Details	TNASE_1 Thermonuclease family signature 1. DGDTIkLmykgqpmtf.RLllVDtPE

Chain	Residue	Details
A	ASP19-GLU43

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site_id	PS01284
Number of Residues	11
Details	TNASE_2 Thermonuclease family signature 2. DKYGRgLAyVY

Chain	Residue	Details
A	ASP83-TYR93

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	ACT_SITE: ACT_SITE => ECO:0000269|PubMed:288045

Chain	Residue	Details
A	ARG35
A	GLU43
A	ARG87

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site_id	SWS_FT_FI2
Number of Residues	3
Details	BINDING:

Chain	Residue	Details
A	ASP21
A	ASP40
A	THR41

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 165

Chain	Residue	Details
A	ASP21	metal ligand
A	ARG35	electrostatic stabiliser, hydrogen bond donor
A	ASP40	metal ligand
A	THR41	metal ligand
A	GLU43	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
A	ARG87	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

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