4R8N
Crystal structure of Staphylococcal nuclease variant V23I/V66I/I72V/I92V at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-04-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 41 |
Unit cell lengths | 47.605, 47.605, 62.912 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.600 - 1.650 |
R-factor | 0.1963 |
Rwork | 0.194 |
R-free | 0.23870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1snc |
RMSD bond length | 0.018 |
RMSD bond angle | 1.887 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.3.0) |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.680 |
High resolution limit [Å] | 1.650 | 4.480 | 1.650 |
Rmerge | 0.041 | 0.044 | 0.274 |
Number of reflections | 16933 | ||
<I/σ(I)> | 20.8 | 12.1 | |
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 13 | 12.3 | 13.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 20% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 277K |