4Q01

Second-site screening of K-Ras in the presence of covalently attached first-site ligands

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003924	molecular_function	GTPase activity
A	0005525	molecular_function	GTP binding
A	0007165	biological_process	signal transduction
A	0016020	cellular_component	membrane
B	0003924	molecular_function	GTPase activity
B	0005525	molecular_function	GTP binding
B	0007165	biological_process	signal transduction
B	0016020	cellular_component	membrane

Functional Information from PDB Data

site_id	AC1
Number of Residues	24
Details	BINDING SITE FOR RESIDUE GDP A 201

Chain	Residue
A	GLY13
A	ASN116
A	LYS117
A	ASP119
A	LEU120
A	SER145
A	ALA146
A	LYS147
A	MG202
A	HOH301
A	HOH302
A	VAL14
A	HOH304
A	HOH307
A	HOH359
A	HOH372
A	HOH412
A	GLY15
A	LYS16
A	SER17
A	ALA18
A	PHE28
A	ASP30
A	GLU31

---

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG A 202

Chain	Residue
A	SER17
A	GDP201
A	HOH301
A	HOH302
A	HOH303
A	HOH304

---

site_id	AC3
Number of Residues	26
Details	BINDING SITE FOR RESIDUE GDP B 201

Chain	Residue
B	GLY13
B	VAL14
B	GLY15
B	LYS16
B	SER17
B	ALA18
B	PHE28
B	VAL29
B	ASP30
B	ASN116
B	LYS117
B	ASP119
B	LEU120
B	SER145
B	ALA146
B	LYS147
B	MG202
B	HOH301
B	HOH303
B	HOH304
B	HOH306
B	HOH325
B	HOH336
B	HOH359
B	HOH374
B	HOH528

---

site_id	AC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE 2XH A 203

Chain	Residue
A	LEU6
A	CYS39
A	ASP54
A	ILE55
A	LEU56
A	TYR71
B	MET67

---

site_id	AC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG B 202

Chain	Residue
B	SER17
B	GDP201
B	HOH301
B	HOH302
B	HOH303
B	HOH304

---

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE 2XH B 203

Chain	Residue
B	LEU6
B	VAL7
B	CYS39
B	ILE55
B	LEU56
B	TYR71

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	16
Details	Motif: {"description":"Effector region"}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	36
Details	Binding site: {"evidences":[{"source":"PubMed","id":"22431598","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"22566140","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"34380736","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"35522713","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Modified residue: {"description":"N-acetylmethionine; in GTPase KRas; alternate","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"FEB-2008","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","Calvo F.","Kolch W."]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Modified residue: {"description":"N-acetylthreonine; in GTPase KRas, N-terminally processed","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"FEB-2008","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","Calvo F.","Kolch W."]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"22711838","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Glycosylation: {"description":"(Microbial infection) O-linked (Glc) threonine; by P.sordellii toxin TcsL","evidences":[{"source":"PubMed","id":"19744486","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---