Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4PKH

Complex of ADP-actin With the N-terminal Actin-Binding Domain of Tropomodulin

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0001725	cellular_component	stress fiber
A	0003785	molecular_function	actin monomer binding
A	0005509	molecular_function	calcium ion binding
A	0005515	molecular_function	protein binding
A	0005523	molecular_function	tropomyosin binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005856	cellular_component	cytoskeleton
A	0005865	cellular_component	striated muscle thin filament
A	0005884	cellular_component	actin filament
A	0010628	biological_process	positive regulation of gene expression
A	0016787	molecular_function	hydrolase activity
A	0019904	molecular_function	protein domain specific binding
A	0030027	cellular_component	lamellipodium
A	0030041	biological_process	actin filament polymerization
A	0030175	cellular_component	filopodium
A	0030240	biological_process	skeletal muscle thin filament assembly
A	0031013	molecular_function	troponin I binding
A	0031432	molecular_function	titin binding
A	0031941	cellular_component	filamentous actin
A	0032036	molecular_function	myosin heavy chain binding
A	0032432	cellular_component	actin filament bundle
A	0042802	molecular_function	identical protein binding
A	0044297	cellular_component	cell body
A	0048306	molecular_function	calcium-dependent protein binding
A	0048741	biological_process	skeletal muscle fiber development
A	0051017	biological_process	actin filament bundle assembly
A	0090131	biological_process	mesenchyme migration
A	0098723	cellular_component	skeletal muscle myofibril
A	0140660	molecular_function	cytoskeletal motor activator activity
B	0005523	molecular_function	tropomyosin binding
B	0051015	molecular_function	actin filament binding
B	0051694	biological_process	pointed-end actin filament capping
D	0000287	molecular_function	magnesium ion binding
D	0001725	cellular_component	stress fiber
D	0003785	molecular_function	actin monomer binding
D	0005509	molecular_function	calcium ion binding
D	0005515	molecular_function	protein binding
D	0005523	molecular_function	tropomyosin binding
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005856	cellular_component	cytoskeleton
D	0005865	cellular_component	striated muscle thin filament
D	0005884	cellular_component	actin filament
D	0010628	biological_process	positive regulation of gene expression
D	0016787	molecular_function	hydrolase activity
D	0019904	molecular_function	protein domain specific binding
D	0030027	cellular_component	lamellipodium
D	0030041	biological_process	actin filament polymerization
D	0030175	cellular_component	filopodium
D	0030240	biological_process	skeletal muscle thin filament assembly
D	0031013	molecular_function	troponin I binding
D	0031432	molecular_function	titin binding
D	0031941	cellular_component	filamentous actin
D	0032036	molecular_function	myosin heavy chain binding
D	0032432	cellular_component	actin filament bundle
D	0042802	molecular_function	identical protein binding
D	0044297	cellular_component	cell body
D	0048306	molecular_function	calcium-dependent protein binding
D	0048741	biological_process	skeletal muscle fiber development
D	0051017	biological_process	actin filament bundle assembly
D	0090131	biological_process	mesenchyme migration
D	0098723	cellular_component	skeletal muscle myofibril
D	0140660	molecular_function	cytoskeletal motor activator activity
E	0005523	molecular_function	tropomyosin binding
E	0051015	molecular_function	actin filament binding
E	0051694	biological_process	pointed-end actin filament capping
F	0000287	molecular_function	magnesium ion binding
F	0001725	cellular_component	stress fiber
F	0003785	molecular_function	actin monomer binding
F	0005509	molecular_function	calcium ion binding
F	0005515	molecular_function	protein binding
F	0005523	molecular_function	tropomyosin binding
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005856	cellular_component	cytoskeleton
F	0005865	cellular_component	striated muscle thin filament
F	0005884	cellular_component	actin filament
F	0010628	biological_process	positive regulation of gene expression
F	0016787	molecular_function	hydrolase activity
F	0019904	molecular_function	protein domain specific binding
F	0030027	cellular_component	lamellipodium
F	0030041	biological_process	actin filament polymerization
F	0030175	cellular_component	filopodium
F	0030240	biological_process	skeletal muscle thin filament assembly
F	0031013	molecular_function	troponin I binding
F	0031432	molecular_function	titin binding
F	0031941	cellular_component	filamentous actin
F	0032036	molecular_function	myosin heavy chain binding
F	0032432	cellular_component	actin filament bundle
F	0042802	molecular_function	identical protein binding
F	0044297	cellular_component	cell body
F	0048306	molecular_function	calcium-dependent protein binding
F	0048741	biological_process	skeletal muscle fiber development
F	0051017	biological_process	actin filament bundle assembly
F	0090131	biological_process	mesenchyme migration
F	0098723	cellular_component	skeletal muscle myofibril
F	0140660	molecular_function	cytoskeletal motor activator activity
G	0005523	molecular_function	tropomyosin binding
G	0051015	molecular_function	actin filament binding
G	0051694	biological_process	pointed-end actin filament capping
I	0000287	molecular_function	magnesium ion binding
I	0001725	cellular_component	stress fiber
I	0003785	molecular_function	actin monomer binding
I	0005509	molecular_function	calcium ion binding
I	0005515	molecular_function	protein binding
I	0005523	molecular_function	tropomyosin binding
I	0005524	molecular_function	ATP binding
I	0005737	cellular_component	cytoplasm
I	0005856	cellular_component	cytoskeleton
I	0005865	cellular_component	striated muscle thin filament
I	0005884	cellular_component	actin filament
I	0010628	biological_process	positive regulation of gene expression
I	0016787	molecular_function	hydrolase activity
I	0019904	molecular_function	protein domain specific binding
I	0030027	cellular_component	lamellipodium
I	0030041	biological_process	actin filament polymerization
I	0030175	cellular_component	filopodium
I	0030240	biological_process	skeletal muscle thin filament assembly
I	0031013	molecular_function	troponin I binding
I	0031432	molecular_function	titin binding
I	0031941	cellular_component	filamentous actin
I	0032036	molecular_function	myosin heavy chain binding
I	0032432	cellular_component	actin filament bundle
I	0042802	molecular_function	identical protein binding
I	0044297	cellular_component	cell body
I	0048306	molecular_function	calcium-dependent protein binding
I	0048741	biological_process	skeletal muscle fiber development
I	0051017	biological_process	actin filament bundle assembly
I	0090131	biological_process	mesenchyme migration
I	0098723	cellular_component	skeletal muscle myofibril
I	0140660	molecular_function	cytoskeletal motor activator activity
J	0005523	molecular_function	tropomyosin binding
J	0051015	molecular_function	actin filament binding
J	0051694	biological_process	pointed-end actin filament capping

Functional Information from PDB Data

site_id	AC1
Number of Residues	18
Details	binding site for residue ADP A 401

Chain	Residue
A	GLY13
A	GLU214
A	GLY301
A	GLY302
A	THR303
A	MET305
A	LYS336
A	CA402
A	HOH511
A	HOH516
A	SER14
A	GLY15
A	LEU16
A	LYS18
A	GLY156
A	ASP157
A	GLY182
A	LYS213

site_id	AC2
Number of Residues	4
Details	binding site for residue CA A 402

Chain	Residue
A	ADP401
A	HOH514
A	HOH516
A	HOH588

site_id	AC3
Number of Residues	5
Details	binding site for residue CA A 403

Chain	Residue
A	GLU167
B	ASP136
B	GLY141
B	ALA143
B	HOH1331

site_id	AC4
Number of Residues	6
Details	binding site for residue CA B 1201

Chain	Residue
B	GLY92
B	ASP93
B	GLU124
B	VAL172
B	HOH1315
B	HOH1345

site_id	AC5
Number of Residues	20
Details	binding site for residue ADP D 401

Chain	Residue
D	GLY13
D	SER14
D	GLY15
D	LEU16
D	LYS18
D	GLY156
D	ASP157
D	GLY182
D	ARG210
D	LYS213
D	GLU214
D	GLY301
D	GLY302
D	THR303
D	MET305
D	TYR306
D	LYS336
D	CA402
D	HOH510
D	HOH524

site_id	AC6
Number of Residues	4
Details	binding site for residue CA D 402

Chain	Residue
D	ADP401
D	HOH510
D	HOH511
D	HOH541

site_id	AC7
Number of Residues	6
Details	binding site for residue CA D 403

Chain	Residue
D	GLU167
D	HOH573
E	ASP136
E	GLY141
E	ALA143
E	HOH1317

site_id	AC8
Number of Residues	6
Details	binding site for residue CA E 1201

Chain	Residue
E	GLY92
E	ASP93
E	GLU124
E	VAL172
E	HOH1316
E	HOH1319

site_id	AC9
Number of Residues	17
Details	binding site for residue ADP F 401

Chain	Residue
F	GLY13
F	SER14
F	GLY15
F	LEU16
F	LYS18
F	GLY156
F	ASP157
F	GLY182
F	LYS213
F	GLU214
F	GLY301
F	GLY302
F	THR303
F	TYR306
F	CA402
F	HOH513
F	HOH531

site_id	AD1
Number of Residues	3
Details	binding site for residue CA F 402

Chain	Residue
F	ASP11
F	VAL339
F	ADP401

site_id	AD2
Number of Residues	4
Details	binding site for residue CA F 403

Chain	Residue
F	GLU167
G	ASP136
G	GLY141
G	ALA143

site_id	AD3
Number of Residues	6
Details	binding site for residue CA G 1201

Chain	Residue
G	GLY92
G	ASP93
G	GLU124
G	VAL172
G	HOH1331
G	HOH1338

site_id	AD4
Number of Residues	17
Details	binding site for residue ADP I 401

Chain	Residue
I	GLY13
I	SER14
I	GLY15
I	LEU16
I	LYS18
I	GLY156
I	ASP157
I	GLY182
I	LYS213
I	GLU214
I	GLY301
I	GLY302
I	THR303
I	TYR306
I	CA402
I	HOH513
I	HOH514

site_id	AD5
Number of Residues	4
Details	binding site for residue CA I 402

Chain	Residue
I	GLN137
I	ADP401
I	HOH510
I	HOH513

site_id	AD6
Number of Residues	4
Details	binding site for residue CA I 403

Chain	Residue
I	GLU167
J	ASP136
J	GLY141
J	ALA143

site_id	AD7
Number of Residues	6
Details	binding site for residue CA J 1201

Chain	Residue
J	GLY92
J	ASP93
J	GLU124
J	VAL172
J	HOH1337
J	HOH1339

Functional Information from PROSITE/UniProt

site_id	PS00406
Number of Residues	11
Details	ACTINS_1 Actins signature 1. YVGDEAQs.KRG

Chain	Residue	Details
A	TYR53-GLY63

site_id	PS00432
Number of Residues	9
Details	ACTINS_2 Actins signature 2. WITKqEYDE

Chain	Residue	Details
A	TRP356-GLU364

site_id	PS01132
Number of Residues	13
Details	ACTINS_ACT_LIKE Actins and actin-related proteins signature. LLTEApLNPkaNR

Chain	Residue	Details
A	LEU104-ARG116

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	16
Details	BINDING: BINDING => ECO:0000269\|PubMed:19666512, ECO:0007744\|PDB:3FFK

Chain	Residue	Details
B	THR132
G	VAL133
G	GLY164
G	PHE176
J	THR132
J	VAL133
J	GLY164
J	PHE176
B	VAL133
B	GLY164
B	PHE176
E	THR132
E	VAL133
E	GLY164
E	PHE176
G	THR132

site_id	SWS_FT_FI2
Number of Residues	4
Details	MOD_RES: Phosphotyrosine; by SRC; in vitro => ECO:0000269\|PubMed:10210201

Chain	Residue	Details
B	GLY126
E	GLY126
G	GLY126
J	GLY126

site_id	SWS_FT_FI3
Number of Residues	8
Details	MOD_RES: Methionine (R)-sulfoxide => ECO:0000250\|UniProtKB:P68134

Chain	Residue	Details
A	MET44
A	MET47
D	MET44
D	MET47
F	MET44
F	MET47
I	MET44
I	MET47

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: N6-malonyllysine => ECO:0000250

Chain	Residue	Details
A	LYS61
D	LYS61
F	LYS61
I	LYS61

site_id	SWS_FT_FI5
Number of Residues	4
Details	MOD_RES: Tele-methylhistidine => ECO:0000269\|PubMed:1150665, ECO:0000269\|PubMed:16905096, ECO:0000269\|PubMed:213279, ECO:0000269\|PubMed:2395459, ECO:0000269\|PubMed:499690, ECO:0007744\|PDB:1ATN, ECO:0007744\|PDB:1NWK

Chain	Residue	Details
A	HIS73
D	HIS73
F	HIS73
I	HIS73

site_id	SWS_FT_FI6
Number of Residues	4
Details	MOD_RES: N6-methyllysine => ECO:0000250\|UniProtKB:P68133

Chain	Residue	Details
A	LYS84
D	LYS84
F	LYS84
I	LYS84

site_id	SWS_FT_FI7
Number of Residues	4
Details	MOD_RES: ADP-ribosylarginine; by SpvB => ECO:0000305\|PubMed:16905096

Chain	Residue	Details
A	ARG177
D	ARG177
F	ARG177
I	ARG177

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)