4PKH

Complex of ADP-actin With the N-terminal Actin-Binding Domain of Tropomodulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-06
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0781
Spacegroup name	P 1 21 1
Unit cell lengths	69.222, 135.136, 140.549
Unit cell angles	90.00, 94.41, 90.00

Refinement procedure

Resolution	44.900 - 2.150
R-factor	0.2749
Rwork	0.274
R-free	0.32620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1eqy
RMSD bond length	0.018
RMSD bond angle	1.729
Data scaling software	HKL-2000
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.000	2.280
High resolution limit [Å]	2.150	2.150
Rmerge	0.077	0.460
Number of reflections	138122
<I/σ(I)>	17.03	2
Completeness [%]	98.7	99.4
Redundancy	5.9	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	0.18 M sodium fluoride, 11% w/v PEG3350, 1% v/v PEG1000, 1% v/v PEG400