4PC7
Elongation factor Tu:Ts complex in a near GTP conformation.
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0003723 | molecular_function | RNA binding |
| A | 0003746 | molecular_function | translation elongation factor activity |
| A | 0003924 | molecular_function | GTPase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005525 | molecular_function | GTP binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006412 | biological_process | translation |
| A | 0006414 | biological_process | translational elongation |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0032045 | cellular_component | guanyl-nucleotide exchange factor complex |
| A | 0046677 | biological_process | response to antibiotic |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0097216 | molecular_function | guanosine tetraphosphate binding |
| C | 0003746 | molecular_function | translation elongation factor activity |
| C | 0005085 | molecular_function | guanyl-nucleotide exchange factor activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0006412 | biological_process | translation |
| C | 0006414 | biological_process | translational elongation |
| C | 0008270 | molecular_function | zinc ion binding |
| C | 0016020 | cellular_component | membrane |
| C | 0032045 | cellular_component | guanyl-nucleotide exchange factor complex |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 18 |
| Details | binding site for residue GNP A 401 |
| Chain | Residue |
| A | VAL20 |
| A | ASN135 |
| A | LYS136 |
| A | ASP138 |
| A | MET139 |
| A | SER173 |
| A | ALA174 |
| A | LEU175 |
| A | MG403 |
| A | HOH501 |
| A | ASP21 |
| A | HIS22 |
| A | GLY23 |
| A | LYS24 |
| A | THR25 |
| A | THR26 |
| A | PRO82 |
| A | GLY83 |
| site_id | AC2 |
| Number of Residues | 21 |
| Details | binding site for residue PUL A 402 |
| Chain | Residue |
| A | ILE92 |
| A | THR93 |
| A | GLN97 |
| A | GLN124 |
| A | VAL125 |
| A | PRO213 |
| A | GLU215 |
| A | THR228 |
| A | GLY229 |
| A | ARG230 |
| A | GLU259 |
| A | PHE261 |
| A | ARG262 |
| A | ASN273 |
| A | VAL274 |
| A | TYR331 |
| A | ARG333 |
| A | THR334 |
| A | ARG373 |
| A | ALA375 |
| A | ARG377 |
| site_id | AC3 |
| Number of Residues | 5 |
| Details | binding site for residue MG A 403 |
| Chain | Residue |
| A | THR25 |
| A | ASP80 |
| A | CYS81 |
| A | GNP401 |
| A | HOH501 |
Functional Information from PROSITE/UniProt
| site_id | PS01126 |
| Number of Residues | 16 |
| Details | EF_TS_1 Elongation factor Ts signature 1. LRerTGaGMmDcKKAL |
| Chain | Residue | Details |
| C | LEU11-LEU26 |
| site_id | PS01127 |
| Number of Residues | 11 |
| Details | EF_TS_2 Elongation factor Ts signature 2. EVNCQTDFVAK |
| Chain | Residue | Details |
| C | GLU74-LYS84 |
| site_id | PS00301 |
| Number of Residues | 16 |
| Details | G_TR_1 Translational (tr)-type guanine nucleotide-binding (G) domain signature. DNapeEKaRGITIntS |
| Chain | Residue | Details |
| A | ASP50-SER65 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 7 |
| Details | Region: {"description":"G1","evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 3 |
| Details | Region: {"description":"G3","evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 3 |
| Details | Region: {"description":"G4","evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | Region: {"description":"G5","evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 9 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"29546243","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5OPD","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"29546243","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5MI3","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5OPD","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"18723842","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"19150849","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24141193","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8416965","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 3 |
| Details | Region: {"description":"Involved in Mg(2+) ion dislocation from EF-Tu","evidences":[{"source":"HAMAP-Rule","id":"MF_00050","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 4 |
| Details | M-CSA 535 |
| Chain | Residue | Details |
| A | ASP21 | electrostatic stabiliser |
| A | LYS24 | electrostatic stabiliser |
| A | THR25 | metal ligand |
| A | HIS84 | electrostatic stabiliser |
