4PC7

Elongation factor Tu:Ts complex in a near GTP conformation.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-02
Detector	MAR CCD 165 mm
Wavelength(s)	1.033
Spacegroup name	P 32 2 1
Unit cell lengths	122.820, 122.820, 173.330
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.500 - 3.600
R-factor	0.2567
Rwork	0.255
R-free	0.28310
RMSD bond length	0.002
RMSD bond angle	0.573
Refinement software	PHENIX ((phenix.refine: dev_1702))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.500	3.700
High resolution limit [Å]	3.500	3.500
Rmerge	0.116	0.538
Number of reflections	17862
<I/σ(I)>	10	2.7
Completeness [%]	99.4	99.8
Redundancy	3.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	292	100mM Tris, 1.8M (NH4)2SO4, 0.5mM GDPNP, 0.15mM pulvomycin, 10mM MgCl2