4PC7
Elongation factor Tu:Ts complex in a near GTP conformation.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-04-02 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.033 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 122.820, 122.820, 173.330 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.500 - 3.600 |
R-factor | 0.2567 |
Rwork | 0.255 |
R-free | 0.28310 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.573 |
Refinement software | PHENIX ((phenix.refine: dev_1702)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.500 | 3.700 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.116 | 0.538 |
Number of reflections | 17862 | |
<I/σ(I)> | 10 | 2.7 |
Completeness [%] | 99.4 | 99.8 |
Redundancy | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | 100mM Tris, 1.8M (NH4)2SO4, 0.5mM GDPNP, 0.15mM pulvomycin, 10mM MgCl2 |