4P5B
Crystal structure of a UMP/dUMP methylase PolB from Streptomyces cacaoi bound with 5-Br dUMP
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0004799 | molecular_function | thymidylate synthase activity |
| A | 0006231 | biological_process | dTMP biosynthetic process |
| A | 0006235 | biological_process | dTTP biosynthetic process |
| A | 0008168 | molecular_function | methyltransferase activity |
| A | 0009165 | biological_process | nucleotide biosynthetic process |
| A | 0016740 | molecular_function | transferase activity |
| A | 0032259 | biological_process | methylation |
| A | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| A | 0050797 | molecular_function | thymidylate synthase (FAD) activity |
| A | 0070402 | molecular_function | NADPH binding |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0004799 | molecular_function | thymidylate synthase activity |
| B | 0006231 | biological_process | dTMP biosynthetic process |
| B | 0006235 | biological_process | dTTP biosynthetic process |
| B | 0008168 | molecular_function | methyltransferase activity |
| B | 0009165 | biological_process | nucleotide biosynthetic process |
| B | 0016740 | molecular_function | transferase activity |
| B | 0032259 | biological_process | methylation |
| B | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| B | 0050797 | molecular_function | thymidylate synthase (FAD) activity |
| B | 0070402 | molecular_function | NADPH binding |
| C | 0000166 | molecular_function | nucleotide binding |
| C | 0004799 | molecular_function | thymidylate synthase activity |
| C | 0006231 | biological_process | dTMP biosynthetic process |
| C | 0006235 | biological_process | dTTP biosynthetic process |
| C | 0008168 | molecular_function | methyltransferase activity |
| C | 0009165 | biological_process | nucleotide biosynthetic process |
| C | 0016740 | molecular_function | transferase activity |
| C | 0032259 | biological_process | methylation |
| C | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| C | 0050797 | molecular_function | thymidylate synthase (FAD) activity |
| C | 0070402 | molecular_function | NADPH binding |
| D | 0000166 | molecular_function | nucleotide binding |
| D | 0004799 | molecular_function | thymidylate synthase activity |
| D | 0006231 | biological_process | dTMP biosynthetic process |
| D | 0006235 | biological_process | dTTP biosynthetic process |
| D | 0008168 | molecular_function | methyltransferase activity |
| D | 0009165 | biological_process | nucleotide biosynthetic process |
| D | 0016740 | molecular_function | transferase activity |
| D | 0032259 | biological_process | methylation |
| D | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| D | 0050797 | molecular_function | thymidylate synthase (FAD) activity |
| D | 0070402 | molecular_function | NADPH binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 23 |
| Details | binding site for residue FAD A 301 |
| Chain | Residue |
| A | ARG86 |
| A | HOH475 |
| B | ASN93 |
| B | GLU94 |
| B | SER96 |
| B | BRU301 |
| B | HOH464 |
| C | SER33 |
| C | HIS61 |
| C | SER63 |
| C | GLU66 |
| A | HIS87 |
| C | ASN182 |
| C | ARG184 |
| C | FAD301 |
| C | HOH469 |
| A | ARG88 |
| A | THR89 |
| A | HIS188 |
| A | LEU192 |
| A | ARG193 |
| A | SER203 |
| A | HOH469 |
| site_id | AC2 |
| Number of Residues | 15 |
| Details | binding site for residue BRU A 302 |
| Chain | Residue |
| A | GLU94 |
| A | GLU95 |
| A | SER96 |
| A | ALA97 |
| A | ARG98 |
| A | TYR99 |
| A | ARG166 |
| A | HOH452 |
| B | ARG82 |
| B | HIS83 |
| B | ARG86 |
| B | ARG193 |
| B | FAD302 |
| B | HOH406 |
| B | HOH410 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 A 303 |
| Chain | Residue |
| A | ARG100 |
| A | HOH449 |
| A | HOH482 |
| A | HOH496 |
| site_id | AC4 |
| Number of Residues | 15 |
| Details | binding site for residue BRU B 301 |
| Chain | Residue |
| A | HIS83 |
| A | ARG86 |
| A | ARG193 |
| A | FAD301 |
| B | MET85 |
| B | GLU94 |
| B | GLU95 |
| B | SER96 |
| B | ALA97 |
| B | ARG98 |
| B | TYR99 |
| B | ARG166 |
| B | HOH408 |
| B | HOH417 |
| B | HOH418 |
| site_id | AC5 |
| Number of Residues | 25 |
| Details | binding site for residue FAD B 302 |
| Chain | Residue |
| A | ASN93 |
| A | GLU94 |
| A | BRU302 |
| B | ARG86 |
| B | HIS87 |
| B | ARG88 |
| B | THR89 |
| B | HIS188 |
| B | LEU192 |
| B | ARG193 |
| B | SER203 |
| B | HIS204 |
| B | HOH448 |
| B | HOH452 |
| B | HOH463 |
| B | HOH482 |
| B | HOH491 |
| D | SER33 |
| D | HIS61 |
| D | SER63 |
| D | ASN182 |
| D | ARG184 |
| D | FAD302 |
| D | HOH464 |
| D | HOH508 |
| site_id | AC6 |
| Number of Residues | 21 |
| Details | binding site for residue FAD C 301 |
| Chain | Residue |
| D | GLU94 |
| D | SER96 |
| D | BRU301 |
| D | HOH442 |
| A | SER33 |
| A | HIS61 |
| A | SER63 |
| A | ASN182 |
| A | ARG184 |
| A | FAD301 |
| C | ARG86 |
| C | HIS87 |
| C | ARG88 |
| C | THR89 |
| C | HIS188 |
| C | LEU192 |
| C | ARG193 |
| C | HOH437 |
| C | HOH461 |
| C | HOH477 |
| D | ASN93 |
| site_id | AC7 |
| Number of Residues | 15 |
| Details | binding site for residue BRU C 302 |
| Chain | Residue |
| C | GLU94 |
| C | GLU95 |
| C | SER96 |
| C | ALA97 |
| C | ARG98 |
| C | TYR99 |
| C | ARG166 |
| C | HOH424 |
| C | HOH470 |
| D | ARG82 |
| D | HIS83 |
| D | ARG86 |
| D | ARG193 |
| D | FAD302 |
| D | HOH421 |
| site_id | AC8 |
| Number of Residues | 14 |
| Details | binding site for residue BRU D 301 |
| Chain | Residue |
| C | ARG82 |
| C | HIS83 |
| C | ARG86 |
| C | ARG193 |
| C | FAD301 |
| D | GLU94 |
| D | GLU95 |
| D | SER96 |
| D | ALA97 |
| D | ARG98 |
| D | TYR99 |
| D | ARG166 |
| D | HOH416 |
| D | HOH417 |
| site_id | AC9 |
| Number of Residues | 22 |
| Details | binding site for residue FAD D 302 |
| Chain | Residue |
| B | SER33 |
| B | HIS61 |
| B | SER63 |
| B | THR89 |
| B | ASN182 |
| B | ARG184 |
| B | FAD302 |
| B | HOH450 |
| C | ASN93 |
| C | GLU94 |
| C | BRU302 |
| C | HOH439 |
| D | ARG86 |
| D | HIS87 |
| D | ARG88 |
| D | HIS188 |
| D | LEU192 |
| D | ARG193 |
| D | HIS204 |
| D | HOH449 |
| D | HOH450 |
| D | HOH473 |
