4P5B
Crystal structure of a UMP/dUMP methylase PolB from Streptomyces cacaoi bound with 5-Br dUMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9798 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 170.788, 59.502, 118.981 |
| Unit cell angles | 90.00, 120.31, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.274 |
| R-factor | 0.1666 |
| Rwork | 0.164 |
| R-free | 0.21554 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.102 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.274 |
| Number of reflections | 46721 |
| <I/σ(I)> | 11 |
| Completeness [%] | 98.0 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M sodium cacodylate, pH 6.5 and 1.4M sodium acetate |
