4OX2

I45T cytosolic phosphoenolpyruvate carboxykinase in complex with beta-sulfopyruvate and GTP

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000287	molecular_function	magnesium ion binding
A	0004550	molecular_function	nucleoside diphosphate kinase activity
A	0004611	molecular_function	phosphoenolpyruvate carboxykinase activity
A	0004613	molecular_function	phosphoenolpyruvate carboxykinase (GTP) activity
A	0005525	molecular_function	GTP binding
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005783	cellular_component	endoplasmic reticulum
A	0005829	cellular_component	cytosol
A	0006006	biological_process	glucose metabolic process
A	0006094	biological_process	gluconeogenesis
A	0006107	biological_process	oxaloacetate metabolic process
A	0006607	biological_process	NLS-bearing protein import into nucleus
A	0006629	biological_process	lipid metabolic process
A	0009617	biological_process	response to bacterium
A	0010447	biological_process	response to acidic pH
A	0014823	biological_process	response to activity
A	0016301	molecular_function	kinase activity
A	0016740	molecular_function	transferase activity
A	0016829	molecular_function	lyase activity
A	0016831	molecular_function	carboxy-lyase activity
A	0017076	molecular_function	purine nucleotide binding
A	0018105	biological_process	peptidyl-serine phosphorylation
A	0019003	molecular_function	GDP binding
A	0019543	biological_process	propionate catabolic process
A	0030145	molecular_function	manganese ion binding
A	0031406	molecular_function	carboxylic acid binding
A	0031667	biological_process	response to nutrient levels
A	0032496	biological_process	response to lipopolysaccharide
A	0032868	biological_process	response to insulin
A	0032869	biological_process	cellular response to insulin stimulus
A	0033993	biological_process	response to lipid
A	0036316	biological_process	SREBP-SCAP complex retention in endoplasmic reticulum
A	0042593	biological_process	glucose homeostasis
A	0042594	biological_process	response to starvation
A	0043382	biological_process	positive regulation of memory T cell differentiation
A	0043648	biological_process	dicarboxylic acid metabolic process
A	0045542	biological_process	positive regulation of cholesterol biosynthetic process
A	0045944	biological_process	positive regulation of transcription by RNA polymerase II
A	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process
A	0046327	biological_process	glycerol biosynthetic process from pyruvate
A	0046872	molecular_function	metal ion binding
A	0046889	biological_process	positive regulation of lipid biosynthetic process
A	0046890	biological_process	regulation of lipid biosynthetic process
A	0051365	biological_process	cellular response to potassium ion starvation
A	0051604	biological_process	protein maturation
A	0070365	biological_process	hepatocyte differentiation
A	0070741	biological_process	response to interleukin-6
A	0071300	biological_process	cellular response to retinoic acid
A	0071320	biological_process	cellular response to cAMP
A	0071332	biological_process	cellular response to fructose stimulus
A	0071333	biological_process	cellular response to glucose stimulus
A	0071347	biological_process	cellular response to interleukin-1
A	0071356	biological_process	cellular response to tumor necrosis factor
A	0071377	biological_process	cellular response to glucagon stimulus
A	0071456	biological_process	cellular response to hypoxia
A	0071474	biological_process	cellular hyperosmotic response
A	0071475	biological_process	cellular hyperosmotic salinity response
A	0071476	biological_process	cellular hypotonic response
A	0071477	biological_process	cellular hypotonic salinity response
A	0071549	biological_process	cellular response to dexamethasone stimulus
A	0072350	biological_process	tricarboxylic acid metabolic process
A	0097403	biological_process	cellular response to raffinose
A	0106264	molecular_function	protein serine kinase activity (using GTP as donor)
A	1904628	biological_process	cellular response to phorbol 13-acetate 12-myristate
A	1904640	biological_process	response to methionine
B	0000166	molecular_function	nucleotide binding
B	0000287	molecular_function	magnesium ion binding
B	0004550	molecular_function	nucleoside diphosphate kinase activity
B	0004611	molecular_function	phosphoenolpyruvate carboxykinase activity
B	0004613	molecular_function	phosphoenolpyruvate carboxykinase (GTP) activity
B	0005525	molecular_function	GTP binding
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005783	cellular_component	endoplasmic reticulum
B	0005829	cellular_component	cytosol
B	0006006	biological_process	glucose metabolic process
B	0006094	biological_process	gluconeogenesis
B	0006107	biological_process	oxaloacetate metabolic process
B	0006607	biological_process	NLS-bearing protein import into nucleus
B	0006629	biological_process	lipid metabolic process
B	0009617	biological_process	response to bacterium
B	0010447	biological_process	response to acidic pH
B	0014823	biological_process	response to activity
B	0016301	molecular_function	kinase activity
B	0016740	molecular_function	transferase activity
B	0016829	molecular_function	lyase activity
B	0016831	molecular_function	carboxy-lyase activity
B	0017076	molecular_function	purine nucleotide binding
B	0018105	biological_process	peptidyl-serine phosphorylation
B	0019003	molecular_function	GDP binding
B	0019543	biological_process	propionate catabolic process
B	0030145	molecular_function	manganese ion binding
B	0031406	molecular_function	carboxylic acid binding
B	0031667	biological_process	response to nutrient levels
B	0032496	biological_process	response to lipopolysaccharide
B	0032868	biological_process	response to insulin
B	0032869	biological_process	cellular response to insulin stimulus
B	0033993	biological_process	response to lipid
B	0036316	biological_process	SREBP-SCAP complex retention in endoplasmic reticulum
B	0042593	biological_process	glucose homeostasis
B	0042594	biological_process	response to starvation
B	0043382	biological_process	positive regulation of memory T cell differentiation
B	0043648	biological_process	dicarboxylic acid metabolic process
B	0045542	biological_process	positive regulation of cholesterol biosynthetic process
B	0045944	biological_process	positive regulation of transcription by RNA polymerase II
B	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process
B	0046327	biological_process	glycerol biosynthetic process from pyruvate
B	0046872	molecular_function	metal ion binding
B	0046889	biological_process	positive regulation of lipid biosynthetic process
B	0046890	biological_process	regulation of lipid biosynthetic process
B	0051365	biological_process	cellular response to potassium ion starvation
B	0051604	biological_process	protein maturation
B	0070365	biological_process	hepatocyte differentiation
B	0070741	biological_process	response to interleukin-6
B	0071300	biological_process	cellular response to retinoic acid
B	0071320	biological_process	cellular response to cAMP
B	0071332	biological_process	cellular response to fructose stimulus
B	0071333	biological_process	cellular response to glucose stimulus
B	0071347	biological_process	cellular response to interleukin-1
B	0071356	biological_process	cellular response to tumor necrosis factor
B	0071377	biological_process	cellular response to glucagon stimulus
B	0071456	biological_process	cellular response to hypoxia
B	0071474	biological_process	cellular hyperosmotic response
B	0071475	biological_process	cellular hyperosmotic salinity response
B	0071476	biological_process	cellular hypotonic response
B	0071477	biological_process	cellular hypotonic salinity response
B	0071549	biological_process	cellular response to dexamethasone stimulus
B	0072350	biological_process	tricarboxylic acid metabolic process
B	0097403	biological_process	cellular response to raffinose
B	0106264	molecular_function	protein serine kinase activity (using GTP as donor)
B	1904628	biological_process	cellular response to phorbol 13-acetate 12-myristate
B	1904640	biological_process	response to methionine

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	binding site for residue MN A 701

Chain	Residue
A	LYS244
A	HIS264
A	ASP311
A	GTP703
A	SPV704

---

site_id	AC2
Number of Residues	6
Details	binding site for residue MN A 702

Chain	Residue
A	HOH893
A	HOH1004
A	THR291
A	ASP310
A	GTP703
A	HOH868

---

site_id	AC3
Number of Residues	25
Details	binding site for residue GTP A 703

Chain	Residue
A	HIS264
A	PRO285
A	SER286
A	ALA287
A	CYS288
A	GLY289
A	LYS290
A	THR291
A	ASN292
A	ASP311
A	VAL335
A	ARG405
A	ARG436
A	TRP516
A	PHE517
A	PHE525
A	GLY529
A	PHE530
A	ASN533
A	MN701
A	MN702
A	SPV704
A	HOH851
A	HOH868
A	HOH893

---

site_id	AC4
Number of Residues	12
Details	binding site for residue SPV A 704

Chain	Residue
A	ARG87
A	GLY237
A	LYS243
A	LYS244
A	HIS264
A	SER286
A	ASP311
A	PHE333
A	ARG405
A	MN701
A	GTP703
A	HOH874

---

site_id	AC5
Number of Residues	2
Details	binding site for residue MN A 705

Chain	Residue
A	GLU63
A	HOH822

---

site_id	AC6
Number of Residues	5
Details	binding site for residue NA A 706

Chain	Residue
A	LEU79
A	ASN208
A	HOH959
A	HOH964
A	HOH974

---

site_id	AC7
Number of Residues	7
Details	binding site for residue 1PE A 707

Chain	Residue
A	ALA447
A	SER449
A	LEU508
A	PRO509
A	LYS510
A	ILE511
A	HOH947

---

site_id	AC8
Number of Residues	6
Details	binding site for residue MN B 701

Chain	Residue
B	THR291
B	ASP310
B	GTP703
B	HOH863
B	HOH872
B	HOH1010

---

site_id	AC9
Number of Residues	5
Details	binding site for residue MN B 702

Chain	Residue
B	LYS244
B	HIS264
B	ASP311
B	GTP703
B	SPV704

---

site_id	AD1
Number of Residues	30
Details	binding site for residue GTP B 703

Chain	Residue
B	HOH886
B	HOH954
B	HOH1011
B	HOH1027
B	HIS264
B	PRO285
B	SER286
B	ALA287
B	CYS288
B	GLY289
B	LYS290
B	THR291
B	ASN292
B	ASP311
B	VAL335
B	PRO337
B	ARG405
B	ARG436
B	TRP516
B	PHE517
B	PHE525
B	GLY529
B	PHE530
B	ASN533
B	MN701
B	MN702
B	SPV704
B	HOH832
B	HOH863
B	HOH872

---

site_id	AD2
Number of Residues	14
Details	binding site for residue SPV B 704

Chain	Residue
B	ARG87
B	GLY237
B	LYS243
B	LYS244
B	HIS264
B	SER286
B	ASP311
B	PHE333
B	ARG405
B	ALA467
B	MN702
B	GTP703
B	HOH850
B	HOH861

---

site_id	AD3
Number of Residues	3
Details	binding site for residue MN B 705

Chain	Residue
B	HIS502
B	GLU607
B	HOH816

---

site_id	AD4
Number of Residues	6
Details	binding site for residue NA B 706

Chain	Residue
B	VAL65
B	LEU79
B	ASN208
B	HOH1013
B	HOH1014
B	HOH1015

---

site_id	AD5
Number of Residues	4
Details	binding site for residue 1PE B 707

Chain	Residue
B	LEU153
B	TRP314
B	LYS316
B	ARG324

---

site_id	AD6
Number of Residues	7
Details	binding site for residue ETX B 708

Chain	Residue
B	ALA447
B	SER449
B	LEU508
B	PRO509
B	LYS510
B	ILE511
B	HOH1005

---

Functional Information from PROSITE/UniProt

site_id	PS00505
Number of Residues	9
Details	PEPCK_GTP Phosphoenolpyruvate carboxykinase (GTP) signature. FPSACGKTN

Chain	Residue	Details
A	PHE284-ASN292

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"evidences":[{"source":"PubMed","id":"23127136","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"2909519","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"17685635","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18772387","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20476774","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"23127136","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24863970","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"18197707","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18772387","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20476774","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"23127136","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24863970","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"PubMed","id":"17685635","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18197707","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18772387","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20476774","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"23127136","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24863970","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"26322521","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"26709450","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28345895","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"31461616","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4YW9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FH0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FH1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FH2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FH3","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FH4","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FH5","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5V97","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5V9F","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5V9G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6P5O","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"17685635","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18197707","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18772387","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20476774","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"23127136","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24863970","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	18
Details	Binding site: {"evidences":[{"source":"PubMed","id":"17685635","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18772387","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20476774","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"23127136","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24863970","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"26322521","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"26709450","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28345895","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4YW9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FH0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FH1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FH2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FH3","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FH4","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5V97","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5V9F","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5V9G","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"17685635","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20476774","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"23127136","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24863970","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"26322521","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"26709450","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28345895","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4YW9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FH0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FH1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FH2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FH3","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FH4","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5V97","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5V9F","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5V9G","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"22673903","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	6
Details	Modified residue: {"description":"N6-acetyllysine; by p300/EP300","evidences":[{"source":"UniProtKB","id":"P35558","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P35558","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	2
Details	Modified residue: {"description":"N6-acetyllysine; by p300/EP300","evidences":[{"source":"UniProtKB","id":"P35558","evidenceCode":"ECO:0000250"},{"source":"PubMed","id":"30193097","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	2
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"UniProtKB","id":"Q16822","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"Q16822","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	5
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"30193097","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	30
Details	Region: {"description":"Omega-loop","evidences":[{"source":"PubMed","id":"28345895","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---