4ODG

Crystal structure of Staphylococcal nuclease variant V23I/V66I/V74I/V99I at cryogenic temperature

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003676	molecular_function	nucleic acid binding
A	0004518	molecular_function	nuclease activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	18
Details	BINDING SITE FOR RESIDUE THP A 201

Chain	Residue
A	ARG35
A	TYR113
A	TYR115
A	CA202
A	HOH302
A	HOH308
A	HOH314
A	HOH326
A	HOH340
A	HOH356
A	LEU36
A	ASP40
A	LYS71
A	ASP83
A	LYS84
A	TYR85
A	ARG87
A	LEU89

---

site_id	AC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA A 202

Chain	Residue
A	ASP21
A	ASP40
A	THR41
A	THP201
A	HOH306
A	HOH308
A	HOH327

---

site_id	AC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE PO4 A 203

Chain	Residue
A	MET32
A	LYS134
A	HOH349

---

Functional Information from PROSITE/UniProt

site_id	PS01123
Number of Residues	25
Details	TNASE_1 Thermonuclease family signature 1. DGDTIkLmykgqpmtf.RLllVDtPE

Chain	Residue	Details
A	ASP19-GLU43

---

site_id	PS01284
Number of Residues	11
Details	TNASE_2 Thermonuclease family signature 2. DKYGRgLAyIY

Chain	Residue	Details
A	ASP83-TYR93

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	ACT_SITE: ACT_SITE => ECO:0000269|PubMed:288045

Chain	Residue	Details
A	ARG35
A	GLU43
A	ARG87

---

site_id	SWS_FT_FI2
Number of Residues	3
Details	BINDING:

Chain	Residue	Details
A	ASP21
A	ASP40
A	THR41

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 165

Chain	Residue	Details
A	ASP21	metal ligand
A	ARG35	electrostatic stabiliser, hydrogen bond donor
A	ASP40	metal ligand
A	THR41	metal ligand
A	GLU43	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
A	ARG87	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

---