Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4O63

Co-enzyme Induced Conformational Changes in Bovine Eye Glyceraldehyde 3-Phosphate Dehydrogenase

Functional Information from GO Data

Chain	GOid	namespace	contents
O	0000226	biological_process	microtubule cytoskeleton organization
O	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
O	0005515	molecular_function	protein binding
O	0005634	cellular_component	nucleus
O	0005737	cellular_component	cytoplasm
O	0005829	cellular_component	cytosol
O	0005856	cellular_component	cytoskeleton
O	0006006	biological_process	glucose metabolic process
O	0006096	biological_process	glycolytic process
O	0006417	biological_process	regulation of translation
O	0006915	biological_process	apoptotic process
O	0008017	molecular_function	microtubule binding
O	0015630	cellular_component	microtubule cytoskeleton
O	0016491	molecular_function	oxidoreductase activity
O	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
O	0016740	molecular_function	transferase activity
O	0032481	biological_process	positive regulation of type I interferon production
O	0035605	molecular_function	peptidyl-cysteine S-nitrosylase activity
O	0035606	biological_process	peptidyl-cysteine S-trans-nitrosylation
O	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
O	0045087	biological_process	innate immune response
O	0050661	molecular_function	NADP binding
O	0050821	biological_process	protein stabilization
O	0051287	molecular_function	NAD binding
O	0051402	biological_process	neuron apoptotic process
O	0097452	cellular_component	GAIT complex
O	0097718	molecular_function	disordered domain specific binding
O	0098850	cellular_component	extrinsic component of synaptic vesicle membrane
O	0099162	biological_process	regulation of neurotransmitter loading into synaptic vesicle
P	0000226	biological_process	microtubule cytoskeleton organization
P	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
P	0005515	molecular_function	protein binding
P	0005634	cellular_component	nucleus
P	0005737	cellular_component	cytoplasm
P	0005829	cellular_component	cytosol
P	0005856	cellular_component	cytoskeleton
P	0006006	biological_process	glucose metabolic process
P	0006096	biological_process	glycolytic process
P	0006417	biological_process	regulation of translation
P	0006915	biological_process	apoptotic process
P	0008017	molecular_function	microtubule binding
P	0015630	cellular_component	microtubule cytoskeleton
P	0016491	molecular_function	oxidoreductase activity
P	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
P	0016740	molecular_function	transferase activity
P	0032481	biological_process	positive regulation of type I interferon production
P	0035605	molecular_function	peptidyl-cysteine S-nitrosylase activity
P	0035606	biological_process	peptidyl-cysteine S-trans-nitrosylation
P	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
P	0045087	biological_process	innate immune response
P	0050661	molecular_function	NADP binding
P	0050821	biological_process	protein stabilization
P	0051287	molecular_function	NAD binding
P	0051402	biological_process	neuron apoptotic process
P	0097452	cellular_component	GAIT complex
P	0097718	molecular_function	disordered domain specific binding
P	0098850	cellular_component	extrinsic component of synaptic vesicle membrane
P	0099162	biological_process	regulation of neurotransmitter loading into synaptic vesicle
Q	0000226	biological_process	microtubule cytoskeleton organization
Q	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
Q	0005515	molecular_function	protein binding
Q	0005634	cellular_component	nucleus
Q	0005737	cellular_component	cytoplasm
Q	0005829	cellular_component	cytosol
Q	0005856	cellular_component	cytoskeleton
Q	0006006	biological_process	glucose metabolic process
Q	0006096	biological_process	glycolytic process
Q	0006417	biological_process	regulation of translation
Q	0006915	biological_process	apoptotic process
Q	0008017	molecular_function	microtubule binding
Q	0015630	cellular_component	microtubule cytoskeleton
Q	0016491	molecular_function	oxidoreductase activity
Q	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
Q	0016740	molecular_function	transferase activity
Q	0032481	biological_process	positive regulation of type I interferon production
Q	0035605	molecular_function	peptidyl-cysteine S-nitrosylase activity
Q	0035606	biological_process	peptidyl-cysteine S-trans-nitrosylation
Q	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
Q	0045087	biological_process	innate immune response
Q	0050661	molecular_function	NADP binding
Q	0050821	biological_process	protein stabilization
Q	0051287	molecular_function	NAD binding
Q	0051402	biological_process	neuron apoptotic process
Q	0097452	cellular_component	GAIT complex
Q	0097718	molecular_function	disordered domain specific binding
Q	0098850	cellular_component	extrinsic component of synaptic vesicle membrane
Q	0099162	biological_process	regulation of neurotransmitter loading into synaptic vesicle
R	0000226	biological_process	microtubule cytoskeleton organization
R	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
R	0005515	molecular_function	protein binding
R	0005634	cellular_component	nucleus
R	0005737	cellular_component	cytoplasm
R	0005829	cellular_component	cytosol
R	0005856	cellular_component	cytoskeleton
R	0006006	biological_process	glucose metabolic process
R	0006096	biological_process	glycolytic process
R	0006417	biological_process	regulation of translation
R	0006915	biological_process	apoptotic process
R	0008017	molecular_function	microtubule binding
R	0015630	cellular_component	microtubule cytoskeleton
R	0016491	molecular_function	oxidoreductase activity
R	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
R	0016740	molecular_function	transferase activity
R	0032481	biological_process	positive regulation of type I interferon production
R	0035605	molecular_function	peptidyl-cysteine S-nitrosylase activity
R	0035606	biological_process	peptidyl-cysteine S-trans-nitrosylation
R	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
R	0045087	biological_process	innate immune response
R	0050661	molecular_function	NADP binding
R	0050821	biological_process	protein stabilization
R	0051287	molecular_function	NAD binding
R	0051402	biological_process	neuron apoptotic process
R	0097452	cellular_component	GAIT complex
R	0097718	molecular_function	disordered domain specific binding
R	0098850	cellular_component	extrinsic component of synaptic vesicle membrane
R	0099162	biological_process	regulation of neurotransmitter loading into synaptic vesicle

Functional Information from PDB Data

site_id	AC1
Number of Residues	28
Details	BINDING SITE FOR RESIDUE NAD P 401

Chain	Residue
P	ASN6
P	PHE34
P	ILE35
P	ARG77
P	SER95
P	THR96
P	GLY97
P	PHE99
P	SER119
P	ALA120
P	CYS149
P	GLY7
P	ALA180
P	ASN313
P	TYR317
P	HOH502
P	HOH508
P	HOH520
P	HOH562
P	HOH567
P	HOH573
P	PHE8
P	GLY9
P	ARG10
P	ILE11
P	ASN31
P	ASP32
P	PRO33

site_id	AC2
Number of Residues	30
Details	BINDING SITE FOR RESIDUE NAD Q 401

Chain	Residue
P	HOH550
Q	ASN6
Q	GLY7
Q	PHE8
Q	GLY9
Q	ARG10
Q	ILE11
Q	ASN31
Q	ASP32
Q	PRO33
Q	PHE34
Q	GLU76
Q	ARG77
Q	SER95
Q	THR96
Q	GLY97
Q	SER119
Q	ALA120
Q	CYS149
Q	ALA180
Q	ASN313
Q	TYR317
Q	HOH507
Q	HOH511
Q	HOH512
Q	HOH543
Q	HOH557
Q	HOH566
Q	HOH569
Q	HOH575

site_id	AC3
Number of Residues	27
Details	BINDING SITE FOR RESIDUE NAD R 401

Chain	Residue
R	ASN6
R	GLY7
R	PHE8
R	GLY9
R	ARG10
R	ILE11
R	ASN31
R	ASP32
R	PRO33
R	PHE34
R	ILE35
R	GLU76
R	ARG77
R	SER95
R	THR96
R	GLY97
R	PHE99
R	SER119
R	ALA120
R	CYS149
R	ALA180
R	ASN313
R	TYR317
R	HOH504
R	HOH538
R	HOH570
R	HOH571

Functional Information from PROSITE/UniProt

site_id	PS00071
Number of Residues	8
Details	GAPDH Glyceraldehyde 3-phosphate dehydrogenase active site. ASCTTNcL

Chain	Residue	Details
P	ALA147-LEU154

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Nucleophile => ECO:0000255\|PROSITE-ProRule:PRU10009

Chain	Residue	Details
P	CYS149
Q	CYS149
R	CYS149
O	CYS149

site_id	SWS_FT_FI2
Number of Residues	20
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P04406

Chain	Residue	Details
P	ARG10
Q	ASN313
R	ARG10
R	ASP32
R	ARG77
R	SER119
R	ASN313
O	ARG10
O	ASP32
O	ARG77
O	SER119
P	ASP32
O	ASN313
P	ARG77
P	SER119
P	ASN313
Q	ARG10
Q	ASP32
Q	ARG77
Q	SER119

site_id	SWS_FT_FI3
Number of Residues	16
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P22513

Chain	Residue	Details
P	SER148
R	THR179
R	THR208
R	ARG231
O	SER148
O	THR179
O	THR208
O	ARG231
P	THR179
P	THR208
P	ARG231
Q	SER148
Q	THR179
Q	THR208
Q	ARG231
R	SER148

site_id	SWS_FT_FI4
Number of Residues	4
Details	SITE: Activates thiol group during catalysis => ECO:0000250\|UniProtKB:P04406

Chain	Residue	Details
P	HIS176
Q	HIS176
R	HIS176
O	HIS176

site_id	SWS_FT_FI5
Number of Residues	20
Details	MOD_RES: N6,N6-dimethyllysine => ECO:0000250\|UniProtKB:P04406

Chain	Residue	Details
P	LYS2
Q	LYS331
R	LYS2
R	LYS63
R	LYS257
R	LYS260
R	LYS331
O	LYS2
O	LYS63
O	LYS257
O	LYS260
P	LYS63
O	LYS331
P	LYS257
P	LYS260
P	LYS331
Q	LYS2
Q	LYS63
Q	LYS257
Q	LYS260

site_id	SWS_FT_FI6
Number of Residues	28
Details	MOD_RES: Deamidated asparagine => ECO:0000250\|UniProtKB:P04406

Chain	Residue	Details
P	ASN6
Q	ASN67
Q	ASN146
Q	ASN152
Q	ASN222
Q	ASN313
R	ASN6
R	ASN61
R	ASN67
R	ASN146
R	ASN152
P	ASN61
R	ASN222
R	ASN313
O	ASN6
O	ASN61
O	ASN67
O	ASN146
O	ASN152
O	ASN222
O	ASN313
P	ASN67
P	ASN146
P	ASN152
P	ASN222
P	ASN313
Q	ASN6
Q	ASN61

site_id	SWS_FT_FI7
Number of Residues	4
Details	MOD_RES: Phosphotyrosine => ECO:0000250\|UniProtKB:P04406

Chain	Residue	Details
P	TYR39
Q	TYR39
R	TYR39
O	TYR39

site_id	SWS_FT_FI8
Number of Residues	12
Details	MOD_RES: N6-acetyllysine => ECO:0000250\|UniProtKB:P04406

Chain	Residue	Details
P	LYS58
O	LYS58
O	LYS216
O	LYS251
P	LYS216
P	LYS251
Q	LYS58
Q	LYS216
Q	LYS251
R	LYS58
R	LYS216
R	LYS251

site_id	SWS_FT_FI9
Number of Residues	32
Details	MOD_RES: Phosphothreonine => ECO:0000250\|UniProtKB:P04406

Chain	Residue	Details
P	THR72
Q	THR150
Q	THR174
Q	THR179
Q	THR181
Q	THR208
Q	THR226
Q	THR234
R	THR72
R	THR150
R	THR174
P	THR150
R	THR179
R	THR181
R	THR208
R	THR226
R	THR234
O	THR72
O	THR150
O	THR174
O	THR179
O	THR181
P	THR174
O	THR208
O	THR226
O	THR234
P	THR179
P	THR181
P	THR208
P	THR226
P	THR234
Q	THR72

site_id	SWS_FT_FI10
Number of Residues	24
Details	MOD_RES: Phosphoserine => ECO:0000250\|UniProtKB:P04406

Chain	Residue	Details
P	SER119
Q	SER238
Q	SER309
Q	SER330
R	SER119
R	SER145
R	SER148
R	SER238
R	SER309
R	SER330
O	SER119
P	SER145
O	SER145
O	SER148
O	SER238
O	SER309
O	SER330
P	SER148
P	SER238
P	SER309
P	SER330
Q	SER119
Q	SER145
Q	SER148

site_id	SWS_FT_FI11
Number of Residues	4
Details	MOD_RES: S-nitrosocysteine; in reversibly inhibited form => ECO:0000250\|UniProtKB:P04797

Chain	Residue	Details
P	CYS149
Q	CYS149
R	CYS149
O	CYS149

site_id	SWS_FT_FI12
Number of Residues	8
Details	MOD_RES: N6-malonyllysine; alternate => ECO:0000250\|UniProtKB:P04406

Chain	Residue	Details
P	LYS191
P	LYS212
Q	LYS191
Q	LYS212
R	LYS191
R	LYS212
O	LYS191
O	LYS212

site_id	SWS_FT_FI13
Number of Residues	4
Details	MOD_RES: N6-acetyllysine; alternate => ECO:0000250\|UniProtKB:P04406

Chain	Residue	Details
P	LYS224
Q	LYS224
R	LYS224
O	LYS224

site_id	SWS_FT_FI14
Number of Residues	4
Details	MOD_RES: S-nitrosocysteine => ECO:0000250\|UniProtKB:P04406

Chain	Residue	Details
P	CYS244
Q	CYS244
R	CYS244
O	CYS244

site_id	SWS_FT_FI15
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0000250\|UniProtKB:P04406

Chain	Residue	Details
P	LYS183
Q	LYS183
R	LYS183
O	LYS183

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)