Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4O1X

Crystal structure of human thymidylate synthase double mutant C195S-Y202C

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
A	0004799	molecular_function	thymidylate synthase activity
A	0005542	molecular_function	folic acid binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005743	cellular_component	mitochondrial inner membrane
A	0005759	cellular_component	mitochondrial matrix
A	0005829	cellular_component	cytosol
A	0006231	biological_process	dTMP biosynthetic process
A	0006235	biological_process	dTTP biosynthetic process
A	0008168	molecular_function	methyltransferase activity
A	0009165	biological_process	nucleotide biosynthetic process
A	0016020	cellular_component	membrane
A	0016740	molecular_function	transferase activity
A	0016741	molecular_function	transferase activity, transferring one-carbon groups
A	0017148	biological_process	negative regulation of translation
A	0032259	biological_process	methylation
A	0035999	biological_process	tetrahydrofolate interconversion
A	0046653	biological_process	tetrahydrofolate metabolic process
A	0071897	biological_process	DNA biosynthetic process
A	1990825	molecular_function	sequence-specific mRNA binding
B	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
B	0004799	molecular_function	thymidylate synthase activity
B	0005542	molecular_function	folic acid binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005743	cellular_component	mitochondrial inner membrane
B	0005759	cellular_component	mitochondrial matrix
B	0005829	cellular_component	cytosol
B	0006231	biological_process	dTMP biosynthetic process
B	0006235	biological_process	dTTP biosynthetic process
B	0008168	molecular_function	methyltransferase activity
B	0009165	biological_process	nucleotide biosynthetic process
B	0016020	cellular_component	membrane
B	0016740	molecular_function	transferase activity
B	0016741	molecular_function	transferase activity, transferring one-carbon groups
B	0017148	biological_process	negative regulation of translation
B	0032259	biological_process	methylation
B	0035999	biological_process	tetrahydrofolate interconversion
B	0046653	biological_process	tetrahydrofolate metabolic process
B	0071897	biological_process	DNA biosynthetic process
B	1990825	molecular_function	sequence-specific mRNA binding
C	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
C	0004799	molecular_function	thymidylate synthase activity
C	0005542	molecular_function	folic acid binding
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005743	cellular_component	mitochondrial inner membrane
C	0005759	cellular_component	mitochondrial matrix
C	0005829	cellular_component	cytosol
C	0006231	biological_process	dTMP biosynthetic process
C	0006235	biological_process	dTTP biosynthetic process
C	0008168	molecular_function	methyltransferase activity
C	0009165	biological_process	nucleotide biosynthetic process
C	0016020	cellular_component	membrane
C	0016740	molecular_function	transferase activity
C	0016741	molecular_function	transferase activity, transferring one-carbon groups
C	0017148	biological_process	negative regulation of translation
C	0032259	biological_process	methylation
C	0035999	biological_process	tetrahydrofolate interconversion
C	0046653	biological_process	tetrahydrofolate metabolic process
C	0071897	biological_process	DNA biosynthetic process
C	1990825	molecular_function	sequence-specific mRNA binding
D	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
D	0004799	molecular_function	thymidylate synthase activity
D	0005542	molecular_function	folic acid binding
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005743	cellular_component	mitochondrial inner membrane
D	0005759	cellular_component	mitochondrial matrix
D	0005829	cellular_component	cytosol
D	0006231	biological_process	dTMP biosynthetic process
D	0006235	biological_process	dTTP biosynthetic process
D	0008168	molecular_function	methyltransferase activity
D	0009165	biological_process	nucleotide biosynthetic process
D	0016020	cellular_component	membrane
D	0016740	molecular_function	transferase activity
D	0016741	molecular_function	transferase activity, transferring one-carbon groups
D	0017148	biological_process	negative regulation of translation
D	0032259	biological_process	methylation
D	0035999	biological_process	tetrahydrofolate interconversion
D	0046653	biological_process	tetrahydrofolate metabolic process
D	0071897	biological_process	DNA biosynthetic process
D	1990825	molecular_function	sequence-specific mRNA binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 401

Chain	Residue
A	ARG175
A	ARG176
C	PRO193
C	ARG215

site_id	AC2
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CL A 404

Chain	Residue
A	ARG274

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO A 405

Chain	Residue
A	ILE178
A	HOH519
A	ARG163
A	VAL164
A	THR167
A	ARG176

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO A 406

Chain	Residue
A	LEU89
A	ILE92
A	ILE288
A	ASP289
A	PHE291

site_id	AC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 A 407

Chain	Residue
A	ARG78
A	PRO305
A	THR306
B	ARG78
B	PRO305
B	THR306

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 408

Chain	Residue
A	PRO193
A	ARG215
C	ARG175
C	ARG176

site_id	AC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 401

Chain	Residue
B	ARG175
B	ARG176
D	ARG215

site_id	AC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO B 402

Chain	Residue
B	ARG163
B	VAL164
B	THR167
B	ARG176
B	ILE178
B	HOH525

site_id	AC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 C 401

Chain	Residue
C	GLU272
C	ARG274
C	HIS304

site_id	BC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 C 402

Chain	Residue
C	ARG147
C	SER151
C	ASP152

site_id	BC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE EDO C 403

Chain	Residue
C	ARG163
C	VAL164
C	ILE178
C	HOH508

site_id	BC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 D 401

Chain	Residue
B	ARG50
D	ARG78
D	PRO305
D	THR306
D	HOH528

site_id	BC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 D 402

Chain	Residue
B	ARG50
B	PRO193
B	ARG215
D	ARG175
D	ARG176
D	HOH534
D	HOH544

site_id	BC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE EDO D 403

Chain	Residue
D	ARG163
D	VAL164
D	THR167
D	ILE178

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Nucleophile => ECO:0000250\|UniProtKB:P0A884

Chain	Residue	Details
D	SER195
B	SER195
C	SER195

site_id	SWS_FT_FI2
Number of Residues	2
Details	BINDING: in other chain => ECO:0000250\|UniProtKB:P0A884

Chain	Residue	Details
D	ARG50
D	ASN226
B	ARG50
B	ASN226
C	ARG50
C	ASN226

site_id	SWS_FT_FI3
Number of Residues	1
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P45352

Chain	Residue	Details
D	ARG175
B	ARG175
C	ARG175

site_id	SWS_FT_FI4
Number of Residues	3
Details	BINDING: in other chain => ECO:0000250\|UniProtKB:P45352

Chain	Residue	Details
D	SER195
D	ARG215
D	HIS256
B	SER195
B	ARG215
B	HIS256
C	SER195
C	ARG215
C	HIS256

site_id	SWS_FT_FI5
Number of Residues	2
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P0A884

Chain	Residue	Details
D	ASP218
D	ALA312
B	ASP218
B	ALA312
C	ASP218
C	ALA312

site_id	SWS_FT_FI6
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:19690332, ECO:0007744\|PubMed:23186163

Chain	Residue	Details
D	SER114
B	SER114
C	SER114

site_id	SWS_FT_FI7
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744\|PubMed:28112733

Chain	Residue	Details
D	LYS287
C	LYS292
C	LYS308
D	LYS292
D	LYS308
B	LYS287
B	LYS292
B	LYS308
C	LYS287

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)