4O1U

Crystal structure of human thymidylate synthase mutant Y202C

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
A	0004799	molecular_function	thymidylate synthase activity
A	0005542	molecular_function	folic acid binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005743	cellular_component	mitochondrial inner membrane
A	0005759	cellular_component	mitochondrial matrix
A	0005829	cellular_component	cytosol
A	0006231	biological_process	dTMP biosynthetic process
A	0006235	biological_process	dTTP biosynthetic process
A	0008168	molecular_function	methyltransferase activity
A	0009165	biological_process	nucleotide biosynthetic process
A	0016020	cellular_component	membrane
A	0016740	molecular_function	transferase activity
A	0016741	molecular_function	transferase activity, transferring one-carbon groups
A	0017148	biological_process	negative regulation of translation
A	0032259	biological_process	methylation
A	0035999	biological_process	tetrahydrofolate interconversion
A	0046653	biological_process	tetrahydrofolate metabolic process
A	0071897	biological_process	DNA biosynthetic process
A	1990825	molecular_function	sequence-specific mRNA binding
B	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
B	0004799	molecular_function	thymidylate synthase activity
B	0005542	molecular_function	folic acid binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005743	cellular_component	mitochondrial inner membrane
B	0005759	cellular_component	mitochondrial matrix
B	0005829	cellular_component	cytosol
B	0006231	biological_process	dTMP biosynthetic process
B	0006235	biological_process	dTTP biosynthetic process
B	0008168	molecular_function	methyltransferase activity
B	0009165	biological_process	nucleotide biosynthetic process
B	0016020	cellular_component	membrane
B	0016740	molecular_function	transferase activity
B	0016741	molecular_function	transferase activity, transferring one-carbon groups
B	0017148	biological_process	negative regulation of translation
B	0032259	biological_process	methylation
B	0035999	biological_process	tetrahydrofolate interconversion
B	0046653	biological_process	tetrahydrofolate metabolic process
B	0071897	biological_process	DNA biosynthetic process
B	1990825	molecular_function	sequence-specific mRNA binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 A 401

Chain	Residue
A	ARG78
A	ARG185
A	LEU189
A	PRO305
A	THR306
B	ARG176
B	HOH559

---

site_id	AC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 A 402

Chain	Residue
A	HIS196
A	ARG215
A	SER216
A	HOH552
B	ARG175
A	ARG50
A	ASN183

---

site_id	AC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO A 403

Chain	Residue
A	GLN36
A	GLN62
A	ALA63
A	VAL223
A	HIS250
A	LEU252
A	HOH590

---

site_id	AC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE EDO A 404

Chain	Residue
A	ASP218
A	HOH555

---

site_id	AC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 406

Chain	Residue
A	ARG274
A	HIS304
A	HOH551

---

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 B 401

Chain	Residue
A	ARG176
B	ARG78
B	ARG185
B	LEU189
B	PRO305
B	THR306

---

site_id	AC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 402

Chain	Residue
B	ARG274
B	HIS304
B	HOH570

---

site_id	AC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 B 403

Chain	Residue
A	ARG175
B	ASN183
B	HIS196
B	ARG215
B	SER216

---

site_id	AC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO B 404

Chain	Residue
B	GLN36
B	GLN62
B	ALA63
B	VAL223
B	HIS250
B	HOH588

---

site_id	BC1
Number of Residues	1
Details	BINDING SITE FOR RESIDUE EDO B 405

Chain	Residue
B	PHE142

---

site_id	BC2
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CL B 406

Chain	Residue
B	ASP218

---

Functional Information from PROSITE/UniProt

site_id	PS00091
Number of Residues	29
Details	THYMIDYLATE_SYNTHASE Thymidylate synthase active site. RriImcaWNprdlplma.....LpPCHalcQFcV

Chain	Residue	Details
A	ARG175-VAL203

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"UniProtKB","id":"P0A884","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	Binding site: {"description":"in other chain","evidences":[{"source":"UniProtKB","id":"P0A884","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P45352","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	12
Details	Binding site: {"description":"in other chain","evidences":[{"source":"UniProtKB","id":"P45352","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P0A884","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---