4NOS
HUMAN INDUCIBLE NITRIC OXIDE SYNTHASE WITH INHIBITOR
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004517 | molecular_function | nitric-oxide synthase activity |
| A | 0006809 | biological_process | nitric oxide biosynthetic process |
| B | 0004517 | molecular_function | nitric-oxide synthase activity |
| B | 0006809 | biological_process | nitric oxide biosynthetic process |
| C | 0004517 | molecular_function | nitric-oxide synthase activity |
| C | 0006809 | biological_process | nitric oxide biosynthetic process |
| D | 0004517 | molecular_function | nitric-oxide synthase activity |
| D | 0006809 | biological_process | nitric oxide biosynthetic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN A 3000 |
| Chain | Residue |
| A | CYS110 |
| A | CYS115 |
| B | CYS110 |
| B | CYS115 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN C 2000 |
| Chain | Residue |
| C | CYS110 |
| C | CYS115 |
| D | CYS110 |
| D | CYS115 |
| site_id | AC3 |
| Number of Residues | 20 |
| Details | BINDING SITE FOR RESIDUE HEM A 510 |
| Chain | Residue |
| A | TRP194 |
| A | ARG199 |
| A | CYS200 |
| A | GLN205 |
| A | SER242 |
| A | PHE369 |
| A | ASN370 |
| A | TRP372 |
| A | GLU377 |
| A | TRP463 |
| A | TYR489 |
| A | TYR491 |
| A | H2B511 |
| A | ITU512 |
| A | HOH3007 |
| A | HOH3103 |
| A | HOH3131 |
| A | HOH3133 |
| A | HOH3203 |
| A | HOH3219 |
| site_id | AC4 |
| Number of Residues | 15 |
| Details | BINDING SITE FOR RESIDUE H2B A 511 |
| Chain | Residue |
| A | SER118 |
| A | ARG381 |
| A | ILE462 |
| A | TRP463 |
| A | HEM510 |
| A | HOH3001 |
| A | HOH3013 |
| A | HOH3017 |
| A | HOH3131 |
| A | HOH3221 |
| B | TRP461 |
| B | PHE476 |
| B | HIS477 |
| B | GLN478 |
| B | GLU479 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ITU A 512 |
| Chain | Residue |
| A | PHE369 |
| A | TRP372 |
| A | GLU377 |
| A | HEM510 |
| site_id | AC6 |
| Number of Residues | 20 |
| Details | BINDING SITE FOR RESIDUE HEM B 1010 |
| Chain | Residue |
| B | TRP194 |
| B | ARG199 |
| B | CYS200 |
| B | GLN205 |
| B | LEU209 |
| B | SER242 |
| B | PHE369 |
| B | ASN370 |
| B | TRP372 |
| B | MET374 |
| B | GLU377 |
| B | TRP463 |
| B | TYR489 |
| B | TYR491 |
| B | H4B1011 |
| B | ITU1012 |
| B | HOH1059 |
| B | HOH1074 |
| B | HOH1143 |
| B | HOH1144 |
| site_id | AC7 |
| Number of Residues | 16 |
| Details | BINDING SITE FOR RESIDUE H4B B 1011 |
| Chain | Residue |
| A | TRP461 |
| A | PHE476 |
| A | HIS477 |
| A | GLN478 |
| A | GLU479 |
| B | SER118 |
| B | MET120 |
| B | ARG381 |
| B | ILE462 |
| B | TRP463 |
| B | HEM1010 |
| B | HOH1018 |
| B | HOH1021 |
| B | HOH1056 |
| B | HOH1113 |
| B | HOH1144 |
| site_id | AC8 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE ITU B 1012 |
| Chain | Residue |
| B | VAL352 |
| B | PHE369 |
| B | TRP372 |
| B | GLU377 |
| B | HEM1010 |
| site_id | AC9 |
| Number of Residues | 19 |
| Details | BINDING SITE FOR RESIDUE HEM C 2010 |
| Chain | Residue |
| C | ASN370 |
| C | TRP372 |
| C | GLU377 |
| C | TRP463 |
| C | TYR491 |
| C | H4B2011 |
| C | ITU2012 |
| C | HOH2041 |
| C | HOH2075 |
| C | HOH2142 |
| C | HOH2161 |
| C | HOH2164 |
| C | HOH2173 |
| C | TRP194 |
| C | ARG199 |
| C | CYS200 |
| C | GLN205 |
| C | SER242 |
| C | PHE369 |
| site_id | BC1 |
| Number of Residues | 16 |
| Details | BINDING SITE FOR RESIDUE H4B C 2011 |
| Chain | Residue |
| C | SER118 |
| C | ARG381 |
| C | ILE462 |
| C | TRP463 |
| C | HEM2010 |
| C | HOH2015 |
| C | HOH2016 |
| C | HOH2044 |
| C | HOH2056 |
| C | HOH2115 |
| C | HOH2142 |
| D | TRP461 |
| D | PHE476 |
| D | HIS477 |
| D | GLN478 |
| D | GLU479 |
| site_id | BC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE ITU C 2012 |
| Chain | Residue |
| C | PRO350 |
| C | PHE369 |
| C | TRP372 |
| C | GLU377 |
| C | HEM2010 |
| C | HOH2214 |
| site_id | BC3 |
| Number of Residues | 20 |
| Details | BINDING SITE FOR RESIDUE HEM D 3010 |
| Chain | Residue |
| D | TRP194 |
| D | ARG199 |
| D | CYS200 |
| D | GLN205 |
| D | SER242 |
| D | PHE369 |
| D | ASN370 |
| D | GLY371 |
| D | TRP372 |
| D | MET374 |
| D | GLU377 |
| D | TRP463 |
| D | TYR489 |
| D | TYR491 |
| D | H4B3011 |
| D | ITU3012 |
| D | HOH3056 |
| D | HOH3060 |
| D | HOH3137 |
| D | HOH3153 |
| site_id | BC4 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE H4B D 3011 |
| Chain | Residue |
| C | TRP461 |
| C | PHE476 |
| C | HIS477 |
| C | GLN478 |
| D | SER118 |
| D | ARG381 |
| D | ILE462 |
| D | TRP463 |
| D | HEM3010 |
| D | HOH3014 |
| D | HOH3021 |
| D | HOH3052 |
| site_id | BC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE ITU D 3012 |
| Chain | Residue |
| D | VAL352 |
| D | PHE369 |
| D | TRP372 |
| D | GLU377 |
| D | HEM3010 |
| site_id | COF |
| Number of Residues | 2 |
| Details | COFACTORS |
| Chain | Residue |
| A | HEM510 |
| A | H2B511 |
| site_id | SUB |
| Number of Residues | 1 |
| Details | INHIBITOR |
| Chain | Residue |
| A | ITU512 |
| site_id | ZNB |
| Number of Residues | 2 |
| Details | ZN BINDING SITE AT THE DIMERIZATION INTERFACE 2 MOTIFS CX4C |
| Chain | Residue |
| A | CYS110 |
| A | CYS115 |
Functional Information from PROSITE/UniProt
| site_id | PS60001 |
| Number of Residues | 8 |
| Details | NOS Nitric oxide synthase (NOS) signature. RCIGRIqW |
| Chain | Residue | Details |
| A | ARG199-TRP206 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 8 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"10074942","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"10409685","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1NSI","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4NOS","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 24 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"10074942","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"10409685","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1NSI","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2NSI","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4NOS","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | Binding site: {"description":"axial binding residue","evidences":[{"source":"PubMed","id":"10074942","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"10409685","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1NSI","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2NSI","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4NOS","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 16 |
| Details | Binding site: {"evidences":[{"source":"UniProtKB","id":"P29474","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | Modified residue: {"description":"Phosphoserine; by PKA","evidences":[{"evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 3nos |
| Chain | Residue | Details |
| A | TRP372 | |
| A | CYS200 | |
| A | GLU377 | |
| A | ARG203 |
| site_id | CSA2 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 3nos |
| Chain | Residue | Details |
| B | TRP372 | |
| B | CYS200 | |
| B | GLU377 | |
| B | ARG203 |
| site_id | CSA3 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 3nos |
| Chain | Residue | Details |
| C | TRP372 | |
| C | CYS200 | |
| C | GLU377 | |
| C | ARG203 |
| site_id | CSA4 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 3nos |
| Chain | Residue | Details |
| D | TRP372 | |
| D | CYS200 | |
| D | GLU377 | |
| D | ARG203 |
