4MD9

Crystal Structure of symmetric CK2 holoenzyme with mutated alpha subunit (F121E truncated at aa 336)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000785	cellular_component	chromatin
A	0003682	molecular_function	chromatin binding
A	0004674	molecular_function	protein serine/threonine kinase activity
A	0005102	molecular_function	signaling receptor binding
A	0005515	molecular_function	protein binding
A	0005576	cellular_component	extracellular region
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0005956	cellular_component	protein kinase CK2 complex
A	0007165	biological_process	signal transduction
A	0008285	biological_process	negative regulation of cell population proliferation
A	0016055	biological_process	Wnt signaling pathway
A	0016605	cellular_component	PML body
A	0018107	biological_process	peptidyl-threonine phosphorylation
A	0019887	molecular_function	protein kinase regulator activity
A	0019904	molecular_function	protein domain specific binding
A	0030674	molecular_function	protein-macromolecule adaptor activity
A	0031519	cellular_component	PcG protein complex
A	0032435	biological_process	negative regulation of proteasomal ubiquitin-dependent protein catabolic process
A	0032927	biological_process	positive regulation of activin receptor signaling pathway
A	0033211	biological_process	adiponectin-activated signaling pathway
A	0034774	cellular_component	secretory granule lumen
A	0042802	molecular_function	identical protein binding
A	0043537	biological_process	negative regulation of blood vessel endothelial cell migration
A	0046872	molecular_function	metal ion binding
A	0051101	biological_process	regulation of DNA binding
A	0060391	biological_process	positive regulation of SMAD protein signal transduction
A	0061154	biological_process	endothelial tube morphogenesis
A	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
A	0065003	biological_process	protein-containing complex assembly
A	0070062	cellular_component	extracellular exosome
A	0075342	biological_process	symbiont-mediated disruption of host cell PML body
A	1903901	biological_process	negative regulation of viral life cycle
A	1904813	cellular_component	ficolin-1-rich granule lumen
B	0000785	cellular_component	chromatin
B	0003682	molecular_function	chromatin binding
B	0004674	molecular_function	protein serine/threonine kinase activity
B	0005102	molecular_function	signaling receptor binding
B	0005515	molecular_function	protein binding
B	0005576	cellular_component	extracellular region
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0005956	cellular_component	protein kinase CK2 complex
B	0007165	biological_process	signal transduction
B	0008285	biological_process	negative regulation of cell population proliferation
B	0016055	biological_process	Wnt signaling pathway
B	0016605	cellular_component	PML body
B	0018107	biological_process	peptidyl-threonine phosphorylation
B	0019887	molecular_function	protein kinase regulator activity
B	0019904	molecular_function	protein domain specific binding
B	0030674	molecular_function	protein-macromolecule adaptor activity
B	0031519	cellular_component	PcG protein complex
B	0032435	biological_process	negative regulation of proteasomal ubiquitin-dependent protein catabolic process
B	0032927	biological_process	positive regulation of activin receptor signaling pathway
B	0033211	biological_process	adiponectin-activated signaling pathway
B	0034774	cellular_component	secretory granule lumen
B	0042802	molecular_function	identical protein binding
B	0043537	biological_process	negative regulation of blood vessel endothelial cell migration
B	0046872	molecular_function	metal ion binding
B	0051101	biological_process	regulation of DNA binding
B	0060391	biological_process	positive regulation of SMAD protein signal transduction
B	0061154	biological_process	endothelial tube morphogenesis
B	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
B	0065003	biological_process	protein-containing complex assembly
B	0070062	cellular_component	extracellular exosome
B	0075342	biological_process	symbiont-mediated disruption of host cell PML body
B	1903901	biological_process	negative regulation of viral life cycle
B	1904813	cellular_component	ficolin-1-rich granule lumen
C	0000785	cellular_component	chromatin
C	0003682	molecular_function	chromatin binding
C	0004674	molecular_function	protein serine/threonine kinase activity
C	0005102	molecular_function	signaling receptor binding
C	0005515	molecular_function	protein binding
C	0005576	cellular_component	extracellular region
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0005956	cellular_component	protein kinase CK2 complex
C	0007165	biological_process	signal transduction
C	0008285	biological_process	negative regulation of cell population proliferation
C	0016055	biological_process	Wnt signaling pathway
C	0016605	cellular_component	PML body
C	0018107	biological_process	peptidyl-threonine phosphorylation
C	0019887	molecular_function	protein kinase regulator activity
C	0019904	molecular_function	protein domain specific binding
C	0030674	molecular_function	protein-macromolecule adaptor activity
C	0031519	cellular_component	PcG protein complex
C	0032435	biological_process	negative regulation of proteasomal ubiquitin-dependent protein catabolic process
C	0032927	biological_process	positive regulation of activin receptor signaling pathway
C	0033211	biological_process	adiponectin-activated signaling pathway
C	0034774	cellular_component	secretory granule lumen
C	0042802	molecular_function	identical protein binding
C	0043537	biological_process	negative regulation of blood vessel endothelial cell migration
C	0046872	molecular_function	metal ion binding
C	0051101	biological_process	regulation of DNA binding
C	0060391	biological_process	positive regulation of SMAD protein signal transduction
C	0061154	biological_process	endothelial tube morphogenesis
C	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
C	0065003	biological_process	protein-containing complex assembly
C	0070062	cellular_component	extracellular exosome
C	0075342	biological_process	symbiont-mediated disruption of host cell PML body
C	1903901	biological_process	negative regulation of viral life cycle
C	1904813	cellular_component	ficolin-1-rich granule lumen
D	0000785	cellular_component	chromatin
D	0003682	molecular_function	chromatin binding
D	0004674	molecular_function	protein serine/threonine kinase activity
D	0005102	molecular_function	signaling receptor binding
D	0005515	molecular_function	protein binding
D	0005576	cellular_component	extracellular region
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0005956	cellular_component	protein kinase CK2 complex
D	0007165	biological_process	signal transduction
D	0008285	biological_process	negative regulation of cell population proliferation
D	0016055	biological_process	Wnt signaling pathway
D	0016605	cellular_component	PML body
D	0018107	biological_process	peptidyl-threonine phosphorylation
D	0019887	molecular_function	protein kinase regulator activity
D	0019904	molecular_function	protein domain specific binding
D	0030674	molecular_function	protein-macromolecule adaptor activity
D	0031519	cellular_component	PcG protein complex
D	0032435	biological_process	negative regulation of proteasomal ubiquitin-dependent protein catabolic process
D	0032927	biological_process	positive regulation of activin receptor signaling pathway
D	0033211	biological_process	adiponectin-activated signaling pathway
D	0034774	cellular_component	secretory granule lumen
D	0042802	molecular_function	identical protein binding
D	0043537	biological_process	negative regulation of blood vessel endothelial cell migration
D	0046872	molecular_function	metal ion binding
D	0051101	biological_process	regulation of DNA binding
D	0060391	biological_process	positive regulation of SMAD protein signal transduction
D	0061154	biological_process	endothelial tube morphogenesis
D	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
D	0065003	biological_process	protein-containing complex assembly
D	0070062	cellular_component	extracellular exosome
D	0075342	biological_process	symbiont-mediated disruption of host cell PML body
D	1903901	biological_process	negative regulation of viral life cycle
D	1904813	cellular_component	ficolin-1-rich granule lumen
E	0004672	molecular_function	protein kinase activity
E	0004674	molecular_function	protein serine/threonine kinase activity
E	0005524	molecular_function	ATP binding
E	0006468	biological_process	protein phosphorylation
F	0004672	molecular_function	protein kinase activity
F	0004674	molecular_function	protein serine/threonine kinase activity
F	0005524	molecular_function	ATP binding
F	0006468	biological_process	protein phosphorylation
G	0004672	molecular_function	protein kinase activity
G	0004674	molecular_function	protein serine/threonine kinase activity
G	0005524	molecular_function	ATP binding
G	0006468	biological_process	protein phosphorylation
H	0004672	molecular_function	protein kinase activity
H	0004674	molecular_function	protein serine/threonine kinase activity
H	0005524	molecular_function	ATP binding
H	0006468	biological_process	protein phosphorylation
I	0000785	cellular_component	chromatin
I	0003682	molecular_function	chromatin binding
I	0004674	molecular_function	protein serine/threonine kinase activity
I	0005102	molecular_function	signaling receptor binding
I	0005515	molecular_function	protein binding
I	0005576	cellular_component	extracellular region
I	0005634	cellular_component	nucleus
I	0005654	cellular_component	nucleoplasm
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0005886	cellular_component	plasma membrane
I	0005956	cellular_component	protein kinase CK2 complex
I	0007165	biological_process	signal transduction
I	0008285	biological_process	negative regulation of cell population proliferation
I	0016055	biological_process	Wnt signaling pathway
I	0016605	cellular_component	PML body
I	0018107	biological_process	peptidyl-threonine phosphorylation
I	0019887	molecular_function	protein kinase regulator activity
I	0019904	molecular_function	protein domain specific binding
I	0030674	molecular_function	protein-macromolecule adaptor activity
I	0031519	cellular_component	PcG protein complex
I	0032435	biological_process	negative regulation of proteasomal ubiquitin-dependent protein catabolic process
I	0032927	biological_process	positive regulation of activin receptor signaling pathway
I	0033211	biological_process	adiponectin-activated signaling pathway
I	0034774	cellular_component	secretory granule lumen
I	0042802	molecular_function	identical protein binding
I	0043537	biological_process	negative regulation of blood vessel endothelial cell migration
I	0046872	molecular_function	metal ion binding
I	0051101	biological_process	regulation of DNA binding
I	0060391	biological_process	positive regulation of SMAD protein signal transduction
I	0061154	biological_process	endothelial tube morphogenesis
I	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
I	0065003	biological_process	protein-containing complex assembly
I	0070062	cellular_component	extracellular exosome
I	0075342	biological_process	symbiont-mediated disruption of host cell PML body
I	1903901	biological_process	negative regulation of viral life cycle
I	1904813	cellular_component	ficolin-1-rich granule lumen
J	0000785	cellular_component	chromatin
J	0003682	molecular_function	chromatin binding
J	0004674	molecular_function	protein serine/threonine kinase activity
J	0005102	molecular_function	signaling receptor binding
J	0005515	molecular_function	protein binding
J	0005576	cellular_component	extracellular region
J	0005634	cellular_component	nucleus
J	0005654	cellular_component	nucleoplasm
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0005886	cellular_component	plasma membrane
J	0005956	cellular_component	protein kinase CK2 complex
J	0007165	biological_process	signal transduction
J	0008285	biological_process	negative regulation of cell population proliferation
J	0016055	biological_process	Wnt signaling pathway
J	0016605	cellular_component	PML body
J	0018107	biological_process	peptidyl-threonine phosphorylation
J	0019887	molecular_function	protein kinase regulator activity
J	0019904	molecular_function	protein domain specific binding
J	0030674	molecular_function	protein-macromolecule adaptor activity
J	0031519	cellular_component	PcG protein complex
J	0032435	biological_process	negative regulation of proteasomal ubiquitin-dependent protein catabolic process
J	0032927	biological_process	positive regulation of activin receptor signaling pathway
J	0033211	biological_process	adiponectin-activated signaling pathway
J	0034774	cellular_component	secretory granule lumen
J	0042802	molecular_function	identical protein binding
J	0043537	biological_process	negative regulation of blood vessel endothelial cell migration
J	0046872	molecular_function	metal ion binding
J	0051101	biological_process	regulation of DNA binding
J	0060391	biological_process	positive regulation of SMAD protein signal transduction
J	0061154	biological_process	endothelial tube morphogenesis
J	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
J	0065003	biological_process	protein-containing complex assembly
J	0070062	cellular_component	extracellular exosome
J	0075342	biological_process	symbiont-mediated disruption of host cell PML body
J	1903901	biological_process	negative regulation of viral life cycle
J	1904813	cellular_component	ficolin-1-rich granule lumen
K	0004672	molecular_function	protein kinase activity
K	0004674	molecular_function	protein serine/threonine kinase activity
K	0005524	molecular_function	ATP binding
K	0006468	biological_process	protein phosphorylation
L	0004672	molecular_function	protein kinase activity
L	0004674	molecular_function	protein serine/threonine kinase activity
L	0005524	molecular_function	ATP binding
L	0006468	biological_process	protein phosphorylation
M	0004672	molecular_function	protein kinase activity
M	0004674	molecular_function	protein serine/threonine kinase activity
M	0005524	molecular_function	ATP binding
M	0006468	biological_process	protein phosphorylation
N	0000785	cellular_component	chromatin
N	0003682	molecular_function	chromatin binding
N	0004674	molecular_function	protein serine/threonine kinase activity
N	0005102	molecular_function	signaling receptor binding
N	0005515	molecular_function	protein binding
N	0005576	cellular_component	extracellular region
N	0005634	cellular_component	nucleus
N	0005654	cellular_component	nucleoplasm
N	0005737	cellular_component	cytoplasm
N	0005829	cellular_component	cytosol
N	0005886	cellular_component	plasma membrane
N	0005956	cellular_component	protein kinase CK2 complex
N	0007165	biological_process	signal transduction
N	0008285	biological_process	negative regulation of cell population proliferation
N	0016055	biological_process	Wnt signaling pathway
N	0016605	cellular_component	PML body
N	0018107	biological_process	peptidyl-threonine phosphorylation
N	0019887	molecular_function	protein kinase regulator activity
N	0019904	molecular_function	protein domain specific binding
N	0030674	molecular_function	protein-macromolecule adaptor activity
N	0031519	cellular_component	PcG protein complex
N	0032435	biological_process	negative regulation of proteasomal ubiquitin-dependent protein catabolic process
N	0032927	biological_process	positive regulation of activin receptor signaling pathway
N	0033211	biological_process	adiponectin-activated signaling pathway
N	0034774	cellular_component	secretory granule lumen
N	0042802	molecular_function	identical protein binding
N	0043537	biological_process	negative regulation of blood vessel endothelial cell migration
N	0046872	molecular_function	metal ion binding
N	0051101	biological_process	regulation of DNA binding
N	0060391	biological_process	positive regulation of SMAD protein signal transduction
N	0061154	biological_process	endothelial tube morphogenesis
N	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
N	0065003	biological_process	protein-containing complex assembly
N	0070062	cellular_component	extracellular exosome
N	0075342	biological_process	symbiont-mediated disruption of host cell PML body
N	1903901	biological_process	negative regulation of viral life cycle
N	1904813	cellular_component	ficolin-1-rich granule lumen
O	0000785	cellular_component	chromatin
O	0003682	molecular_function	chromatin binding
O	0004674	molecular_function	protein serine/threonine kinase activity
O	0005102	molecular_function	signaling receptor binding
O	0005515	molecular_function	protein binding
O	0005576	cellular_component	extracellular region
O	0005634	cellular_component	nucleus
O	0005654	cellular_component	nucleoplasm
O	0005737	cellular_component	cytoplasm
O	0005829	cellular_component	cytosol
O	0005886	cellular_component	plasma membrane
O	0005956	cellular_component	protein kinase CK2 complex
O	0007165	biological_process	signal transduction
O	0008285	biological_process	negative regulation of cell population proliferation
O	0016055	biological_process	Wnt signaling pathway
O	0016605	cellular_component	PML body
O	0018107	biological_process	peptidyl-threonine phosphorylation
O	0019887	molecular_function	protein kinase regulator activity
O	0019904	molecular_function	protein domain specific binding
O	0030674	molecular_function	protein-macromolecule adaptor activity
O	0031519	cellular_component	PcG protein complex
O	0032435	biological_process	negative regulation of proteasomal ubiquitin-dependent protein catabolic process
O	0032927	biological_process	positive regulation of activin receptor signaling pathway
O	0033211	biological_process	adiponectin-activated signaling pathway
O	0034774	cellular_component	secretory granule lumen
O	0042802	molecular_function	identical protein binding
O	0043537	biological_process	negative regulation of blood vessel endothelial cell migration
O	0046872	molecular_function	metal ion binding
O	0051101	biological_process	regulation of DNA binding
O	0060391	biological_process	positive regulation of SMAD protein signal transduction
O	0061154	biological_process	endothelial tube morphogenesis
O	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
O	0065003	biological_process	protein-containing complex assembly
O	0070062	cellular_component	extracellular exosome
O	0075342	biological_process	symbiont-mediated disruption of host cell PML body
O	1903901	biological_process	negative regulation of viral life cycle
O	1904813	cellular_component	ficolin-1-rich granule lumen
P	0004672	molecular_function	protein kinase activity
P	0004674	molecular_function	protein serine/threonine kinase activity
P	0005524	molecular_function	ATP binding
P	0006468	biological_process	protein phosphorylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 301

Chain	Residue
A	CYS109
A	CYS114
A	CYS137
A	CYS140

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN B 301

Chain	Residue
B	CYS109
B	CYS114
B	CYS137
B	CYS140

---

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN C 301

Chain	Residue
C	CYS114
C	CYS137
C	CYS140
C	CYS109

---

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN D 301

Chain	Residue
D	CYS109
D	CYS114
D	CYS137
D	CYS140

---

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN I 301

Chain	Residue
I	CYS109
I	CYS114
I	CYS137
I	CYS140

---

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN J 301

Chain	Residue
J	CYS109
J	CYS114
J	CYS137
J	CYS140

---

site_id	AC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN N 301

Chain	Residue
N	CYS109
N	CYS114
N	CYS137
N	CYS140

---

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN O 301

Chain	Residue
O	CYS109
O	CYS114
O	CYS137
O	CYS140

---

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	24
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGRGKYSEVFeAinitnnek..........VVVK

Chain	Residue	Details
E	LEU45-LYS68

---

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. ImHrDVKphNVMI

Chain	Residue	Details
E	ILE152-ILE164

---

site_id	PS01101
Number of Residues	32
Details	CK2_BETA Casein kinase II regulatory subunit signature. CPrVyCenqpmLPIGlsdipgeamVKlyCPkC

Chain	Residue	Details
A	CYS109-CYS140

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: Proton acceptor

Chain	Residue	Details
E	ASP156
C	CYS114
C	CYS137
C	CYS140
D	CYS109
D	CYS114
D	CYS137
D	CYS140
I	CYS109
I	CYS114
I	CYS137
F	ASP156
I	CYS140
J	CYS109
J	CYS114
J	CYS137
J	CYS140
N	CYS109
N	CYS114
N	CYS137
N	CYS140
O	CYS109
G	ASP156
O	CYS114
O	CYS137
O	CYS140
H	ASP156
K	ASP156
L	ASP156
M	ASP156
P	ASP156
C	CYS109

---

site_id	SWS_FT_FI2
Number of Residues	16
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00159

Chain	Residue	Details
E	LEU45
K	LYS68
L	LEU45
L	LYS68
M	LEU45
M	LYS68
P	LEU45
P	LYS68
E	LYS68
F	LEU45
F	LYS68
G	LEU45
G	LYS68
H	LEU45
H	LYS68
K	LEU45

---

site_id	SWS_FT_FI3
Number of Residues	16
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18691976

Chain	Residue	Details
A	SER8
I	SER69
J	SER8
J	SER69
N	SER8
N	SER69
O	SER8
O	SER69
A	SER69
B	SER8
B	SER69
C	SER8
C	SER69
D	SER8
D	SER69
I	SER8

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
A	THR37
B	THR37
C	THR37
D	THR37
I	THR37
J	THR37
N	THR37
O	THR37

---

site_id	SWS_FT_FI5
Number of Residues	8
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18691976, ECO:0007744|PubMed:19369195, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163

Chain	Residue	Details
A	SER209
B	SER209
C	SER209
D	SER209
I	SER209
J	SER209
N	SER209
O	SER209

---

site_id	SWS_FT_FI6
Number of Residues	8
Details	MOD_RES: N6-acetyllysine; alternate => ECO:0007744|PubMed:19608861

Chain	Residue	Details
A	LYS212
B	LYS212
C	LYS212
D	LYS212
I	LYS212
J	LYS212
N	LYS212
O	LYS212

---

site_id	SWS_FT_FI7
Number of Residues	16
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate => ECO:0007744|PubMed:25755297

Chain	Residue	Details
A	LYS212
J	LYS212
N	LYS212
O	LYS212
B	LYS212
C	LYS212
D	LYS212
I	LYS212

---