4MD9
Crystal Structure of symmetric CK2 holoenzyme with mutated alpha subunit (F121E truncated at aa 336)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-09 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.873 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 283.924, 115.707, 208.223 |
Unit cell angles | 90.00, 119.22, 90.00 |
Refinement procedure
Resolution | 181.725 - 3.500 |
R-factor | 0.23038 |
Rwork | 0.229 |
R-free | 0.25897 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 4DGL AND 4KWP |
RMSD bond length | 0.009 |
RMSD bond angle | 1.159 |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 181.725 | 48.306 | 3.690 |
High resolution limit [Å] | 3.500 | 11.070 | 3.500 |
Rmerge | 0.039 | 0.621 | |
Number of reflections | 74256 | ||
<I/σ(I)> | 6.6 | 12.5 | 1.2 |
Completeness [%] | 99.7 | 97.2 | 99.9 |
Redundancy | 3.3 | 3.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 277 | 20% PEG3350, 0.2 M ammonium citrate, pH 6.5, VAPOR DIFFUSION, temperature 277K |