Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4MD8

Crystal Structure of full-length symmetric CK2 holoenzyme with mutated alpha subunit (F121E)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000785	cellular_component	chromatin
A	0003682	molecular_function	chromatin binding
A	0004674	molecular_function	protein serine/threonine kinase activity
A	0005102	molecular_function	signaling receptor binding
A	0005515	molecular_function	protein binding
A	0005576	cellular_component	extracellular region
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0005956	cellular_component	protein kinase CK2 complex
A	0007165	biological_process	signal transduction
A	0008285	biological_process	negative regulation of cell population proliferation
A	0016055	biological_process	Wnt signaling pathway
A	0016605	cellular_component	PML body
A	0018107	biological_process	peptidyl-threonine phosphorylation
A	0019887	molecular_function	protein kinase regulator activity
A	0019904	molecular_function	protein domain specific binding
A	0030674	molecular_function	protein-macromolecule adaptor activity
A	0031519	cellular_component	PcG protein complex
A	0032435	biological_process	negative regulation of proteasomal ubiquitin-dependent protein catabolic process
A	0032927	biological_process	positive regulation of activin receptor signaling pathway
A	0033211	biological_process	adiponectin-activated signaling pathway
A	0034774	cellular_component	secretory granule lumen
A	0042802	molecular_function	identical protein binding
A	0043537	biological_process	negative regulation of blood vessel endothelial cell migration
A	0046872	molecular_function	metal ion binding
A	0051101	biological_process	regulation of DNA binding
A	0060391	biological_process	positive regulation of SMAD protein signal transduction
A	0061154	biological_process	endothelial tube morphogenesis
A	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
A	0065003	biological_process	protein-containing complex assembly
A	0070062	cellular_component	extracellular exosome
A	0075342	biological_process	symbiont-mediated disruption of host cell PML body
A	1903901	biological_process	negative regulation of viral life cycle
A	1904813	cellular_component	ficolin-1-rich granule lumen
B	0000785	cellular_component	chromatin
B	0003682	molecular_function	chromatin binding
B	0004674	molecular_function	protein serine/threonine kinase activity
B	0005102	molecular_function	signaling receptor binding
B	0005515	molecular_function	protein binding
B	0005576	cellular_component	extracellular region
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0005956	cellular_component	protein kinase CK2 complex
B	0007165	biological_process	signal transduction
B	0008285	biological_process	negative regulation of cell population proliferation
B	0016055	biological_process	Wnt signaling pathway
B	0016605	cellular_component	PML body
B	0018107	biological_process	peptidyl-threonine phosphorylation
B	0019887	molecular_function	protein kinase regulator activity
B	0019904	molecular_function	protein domain specific binding
B	0030674	molecular_function	protein-macromolecule adaptor activity
B	0031519	cellular_component	PcG protein complex
B	0032435	biological_process	negative regulation of proteasomal ubiquitin-dependent protein catabolic process
B	0032927	biological_process	positive regulation of activin receptor signaling pathway
B	0033211	biological_process	adiponectin-activated signaling pathway
B	0034774	cellular_component	secretory granule lumen
B	0042802	molecular_function	identical protein binding
B	0043537	biological_process	negative regulation of blood vessel endothelial cell migration
B	0046872	molecular_function	metal ion binding
B	0051101	biological_process	regulation of DNA binding
B	0060391	biological_process	positive regulation of SMAD protein signal transduction
B	0061154	biological_process	endothelial tube morphogenesis
B	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
B	0065003	biological_process	protein-containing complex assembly
B	0070062	cellular_component	extracellular exosome
B	0075342	biological_process	symbiont-mediated disruption of host cell PML body
B	1903901	biological_process	negative regulation of viral life cycle
B	1904813	cellular_component	ficolin-1-rich granule lumen
C	0000785	cellular_component	chromatin
C	0003682	molecular_function	chromatin binding
C	0004674	molecular_function	protein serine/threonine kinase activity
C	0005102	molecular_function	signaling receptor binding
C	0005515	molecular_function	protein binding
C	0005576	cellular_component	extracellular region
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0005956	cellular_component	protein kinase CK2 complex
C	0007165	biological_process	signal transduction
C	0008285	biological_process	negative regulation of cell population proliferation
C	0016055	biological_process	Wnt signaling pathway
C	0016605	cellular_component	PML body
C	0018107	biological_process	peptidyl-threonine phosphorylation
C	0019887	molecular_function	protein kinase regulator activity
C	0019904	molecular_function	protein domain specific binding
C	0030674	molecular_function	protein-macromolecule adaptor activity
C	0031519	cellular_component	PcG protein complex
C	0032435	biological_process	negative regulation of proteasomal ubiquitin-dependent protein catabolic process
C	0032927	biological_process	positive regulation of activin receptor signaling pathway
C	0033211	biological_process	adiponectin-activated signaling pathway
C	0034774	cellular_component	secretory granule lumen
C	0042802	molecular_function	identical protein binding
C	0043537	biological_process	negative regulation of blood vessel endothelial cell migration
C	0046872	molecular_function	metal ion binding
C	0051101	biological_process	regulation of DNA binding
C	0060391	biological_process	positive regulation of SMAD protein signal transduction
C	0061154	biological_process	endothelial tube morphogenesis
C	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
C	0065003	biological_process	protein-containing complex assembly
C	0070062	cellular_component	extracellular exosome
C	0075342	biological_process	symbiont-mediated disruption of host cell PML body
C	1903901	biological_process	negative regulation of viral life cycle
C	1904813	cellular_component	ficolin-1-rich granule lumen
D	0000785	cellular_component	chromatin
D	0003682	molecular_function	chromatin binding
D	0004674	molecular_function	protein serine/threonine kinase activity
D	0005102	molecular_function	signaling receptor binding
D	0005515	molecular_function	protein binding
D	0005576	cellular_component	extracellular region
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0005956	cellular_component	protein kinase CK2 complex
D	0007165	biological_process	signal transduction
D	0008285	biological_process	negative regulation of cell population proliferation
D	0016055	biological_process	Wnt signaling pathway
D	0016605	cellular_component	PML body
D	0018107	biological_process	peptidyl-threonine phosphorylation
D	0019887	molecular_function	protein kinase regulator activity
D	0019904	molecular_function	protein domain specific binding
D	0030674	molecular_function	protein-macromolecule adaptor activity
D	0031519	cellular_component	PcG protein complex
D	0032435	biological_process	negative regulation of proteasomal ubiquitin-dependent protein catabolic process
D	0032927	biological_process	positive regulation of activin receptor signaling pathway
D	0033211	biological_process	adiponectin-activated signaling pathway
D	0034774	cellular_component	secretory granule lumen
D	0042802	molecular_function	identical protein binding
D	0043537	biological_process	negative regulation of blood vessel endothelial cell migration
D	0046872	molecular_function	metal ion binding
D	0051101	biological_process	regulation of DNA binding
D	0060391	biological_process	positive regulation of SMAD protein signal transduction
D	0061154	biological_process	endothelial tube morphogenesis
D	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
D	0065003	biological_process	protein-containing complex assembly
D	0070062	cellular_component	extracellular exosome
D	0075342	biological_process	symbiont-mediated disruption of host cell PML body
D	1903901	biological_process	negative regulation of viral life cycle
D	1904813	cellular_component	ficolin-1-rich granule lumen
E	0004672	molecular_function	protein kinase activity
E	0004674	molecular_function	protein serine/threonine kinase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0005956	cellular_component	protein kinase CK2 complex
E	0006302	biological_process	double-strand break repair
E	0006468	biological_process	protein phosphorylation
E	0006915	biological_process	apoptotic process
E	0006974	biological_process	DNA damage response
E	0007165	biological_process	signal transduction
E	0008284	biological_process	positive regulation of cell population proliferation
E	0016055	biological_process	Wnt signaling pathway
E	0016301	molecular_function	kinase activity
E	0016605	cellular_component	PML body
E	0017148	biological_process	negative regulation of translation
E	0018105	biological_process	peptidyl-serine phosphorylation
E	0018107	biological_process	peptidyl-threonine phosphorylation
E	0030177	biological_process	positive regulation of Wnt signaling pathway
E	0030307	biological_process	positive regulation of cell growth
E	0031519	cellular_component	PcG protein complex
E	0032435	biological_process	negative regulation of proteasomal ubiquitin-dependent protein catabolic process
E	0042802	molecular_function	identical protein binding
E	0043154	biological_process	negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
E	0045732	biological_process	positive regulation of protein catabolic process
E	0048511	biological_process	rhythmic process
E	0050821	biological_process	protein stabilization
E	0051726	biological_process	regulation of cell cycle
E	0051879	molecular_function	Hsp90 protein binding
E	0061077	biological_process	chaperone-mediated protein folding
E	0070822	cellular_component	Sin3-type complex
E	0075342	biological_process	symbiont-mediated disruption of host cell PML body
E	0106310	molecular_function	protein serine kinase activity
E	1905337	biological_process	positive regulation of aggrephagy
E	1905818	biological_process	regulation of chromosome separation
E	2000042	biological_process	negative regulation of double-strand break repair via homologous recombination
E	2001234	biological_process	negative regulation of apoptotic signaling pathway
F	0004672	molecular_function	protein kinase activity
F	0004674	molecular_function	protein serine/threonine kinase activity
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005829	cellular_component	cytosol
F	0005886	cellular_component	plasma membrane
F	0005956	cellular_component	protein kinase CK2 complex
F	0006302	biological_process	double-strand break repair
F	0006468	biological_process	protein phosphorylation
F	0006915	biological_process	apoptotic process
F	0006974	biological_process	DNA damage response
F	0007165	biological_process	signal transduction
F	0008284	biological_process	positive regulation of cell population proliferation
F	0016055	biological_process	Wnt signaling pathway
F	0016301	molecular_function	kinase activity
F	0016605	cellular_component	PML body
F	0017148	biological_process	negative regulation of translation
F	0018105	biological_process	peptidyl-serine phosphorylation
F	0018107	biological_process	peptidyl-threonine phosphorylation
F	0030177	biological_process	positive regulation of Wnt signaling pathway
F	0030307	biological_process	positive regulation of cell growth
F	0031519	cellular_component	PcG protein complex
F	0032435	biological_process	negative regulation of proteasomal ubiquitin-dependent protein catabolic process
F	0042802	molecular_function	identical protein binding
F	0043154	biological_process	negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
F	0045732	biological_process	positive regulation of protein catabolic process
F	0048511	biological_process	rhythmic process
F	0050821	biological_process	protein stabilization
F	0051726	biological_process	regulation of cell cycle
F	0051879	molecular_function	Hsp90 protein binding
F	0061077	biological_process	chaperone-mediated protein folding
F	0070822	cellular_component	Sin3-type complex
F	0075342	biological_process	symbiont-mediated disruption of host cell PML body
F	0106310	molecular_function	protein serine kinase activity
F	1905337	biological_process	positive regulation of aggrephagy
F	1905818	biological_process	regulation of chromosome separation
F	2000042	biological_process	negative regulation of double-strand break repair via homologous recombination
F	2001234	biological_process	negative regulation of apoptotic signaling pathway
G	0004672	molecular_function	protein kinase activity
G	0004674	molecular_function	protein serine/threonine kinase activity
G	0005515	molecular_function	protein binding
G	0005524	molecular_function	ATP binding
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005829	cellular_component	cytosol
G	0005886	cellular_component	plasma membrane
G	0005956	cellular_component	protein kinase CK2 complex
G	0006302	biological_process	double-strand break repair
G	0006468	biological_process	protein phosphorylation
G	0006915	biological_process	apoptotic process
G	0006974	biological_process	DNA damage response
G	0007165	biological_process	signal transduction
G	0008284	biological_process	positive regulation of cell population proliferation
G	0016055	biological_process	Wnt signaling pathway
G	0016301	molecular_function	kinase activity
G	0016605	cellular_component	PML body
G	0017148	biological_process	negative regulation of translation
G	0018105	biological_process	peptidyl-serine phosphorylation
G	0018107	biological_process	peptidyl-threonine phosphorylation
G	0030177	biological_process	positive regulation of Wnt signaling pathway
G	0030307	biological_process	positive regulation of cell growth
G	0031519	cellular_component	PcG protein complex
G	0032435	biological_process	negative regulation of proteasomal ubiquitin-dependent protein catabolic process
G	0042802	molecular_function	identical protein binding
G	0043154	biological_process	negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
G	0045732	biological_process	positive regulation of protein catabolic process
G	0048511	biological_process	rhythmic process
G	0050821	biological_process	protein stabilization
G	0051726	biological_process	regulation of cell cycle
G	0051879	molecular_function	Hsp90 protein binding
G	0061077	biological_process	chaperone-mediated protein folding
G	0070822	cellular_component	Sin3-type complex
G	0075342	biological_process	symbiont-mediated disruption of host cell PML body
G	0106310	molecular_function	protein serine kinase activity
G	1905337	biological_process	positive regulation of aggrephagy
G	1905818	biological_process	regulation of chromosome separation
G	2000042	biological_process	negative regulation of double-strand break repair via homologous recombination
G	2001234	biological_process	negative regulation of apoptotic signaling pathway
H	0004672	molecular_function	protein kinase activity
H	0004674	molecular_function	protein serine/threonine kinase activity
H	0005515	molecular_function	protein binding
H	0005524	molecular_function	ATP binding
H	0005634	cellular_component	nucleus
H	0005654	cellular_component	nucleoplasm
H	0005829	cellular_component	cytosol
H	0005886	cellular_component	plasma membrane
H	0005956	cellular_component	protein kinase CK2 complex
H	0006302	biological_process	double-strand break repair
H	0006468	biological_process	protein phosphorylation
H	0006915	biological_process	apoptotic process
H	0006974	biological_process	DNA damage response
H	0007165	biological_process	signal transduction
H	0008284	biological_process	positive regulation of cell population proliferation
H	0016055	biological_process	Wnt signaling pathway
H	0016301	molecular_function	kinase activity
H	0016605	cellular_component	PML body
H	0017148	biological_process	negative regulation of translation
H	0018105	biological_process	peptidyl-serine phosphorylation
H	0018107	biological_process	peptidyl-threonine phosphorylation
H	0030177	biological_process	positive regulation of Wnt signaling pathway
H	0030307	biological_process	positive regulation of cell growth
H	0031519	cellular_component	PcG protein complex
H	0032435	biological_process	negative regulation of proteasomal ubiquitin-dependent protein catabolic process
H	0042802	molecular_function	identical protein binding
H	0043154	biological_process	negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
H	0045732	biological_process	positive regulation of protein catabolic process
H	0048511	biological_process	rhythmic process
H	0050821	biological_process	protein stabilization
H	0051726	biological_process	regulation of cell cycle
H	0051879	molecular_function	Hsp90 protein binding
H	0061077	biological_process	chaperone-mediated protein folding
H	0070822	cellular_component	Sin3-type complex
H	0075342	biological_process	symbiont-mediated disruption of host cell PML body
H	0106310	molecular_function	protein serine kinase activity
H	1905337	biological_process	positive regulation of aggrephagy
H	1905818	biological_process	regulation of chromosome separation
H	2000042	biological_process	negative regulation of double-strand break repair via homologous recombination
H	2001234	biological_process	negative regulation of apoptotic signaling pathway

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 301

Chain	Residue
A	CYS109
A	CYS114
A	CYS137
A	CYS140

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN B 301

Chain	Residue
B	CYS109
B	CYS114
B	CYS137
B	CYS140

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN C 301

Chain	Residue
C	CYS114
C	CYS137
C	CYS140
C	CYS109

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN D 301

Chain	Residue
D	CYS109
D	CYS114
D	CYS137
D	CYS140

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	24
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGRGKYSEVFeAinitnnek..........VVVK

Chain	Residue	Details
E	LEU45-LYS68

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. ImHrDVKphNVMI

Chain	Residue	Details
E	ILE152-ILE164

site_id	PS01101
Number of Residues	32
Details	CK2_BETA Casein kinase II regulatory subunit signature. CPrVyCenqpmLPIGlsdipgeamVKlyCPkC

Chain	Residue	Details
A	CYS109-CYS140

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Proton acceptor

Chain	Residue	Details
E	ASP156
C	CYS114
C	CYS137
C	CYS140
D	CYS109
D	CYS114
D	CYS137
D	CYS140
F	ASP156
G	ASP156
H	ASP156
B	CYS109
B	CYS114
B	CYS137
B	CYS140
C	CYS109

site_id	SWS_FT_FI2
Number of Residues	8
Details	BINDING: BINDING => ECO:0000255\|PROSITE-ProRule:PRU00159

Chain	Residue	Details
E	LEU45
E	LYS68
F	LEU45
F	LYS68
G	LEU45
G	LYS68
H	LEU45
H	LYS68

site_id	SWS_FT_FI3
Number of Residues	8
Details	MOD_RES: Phosphothreonine; by CDK1 => ECO:0000269\|PubMed:7592773

Chain	Residue	Details
E	THR344
E	THR360
F	THR344
F	THR360
G	THR344
G	THR360
H	THR344
H	THR360

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: Phosphoserine; by CDK1 => ECO:0000269\|PubMed:7592773

Chain	Residue	Details
E	SER362
F	SER362
G	SER362
H	SER362

site_id	SWS_FT_FI5
Number of Residues	4
Details	MOD_RES: Phosphoserine; by CDK1 => ECO:0000269\|PubMed:7592773, ECO:0007744\|PubMed:18691976

Chain	Residue	Details
E	SER370
F	SER370
G	SER370
H	SER370

site_id	SWS_FT_FI6
Number of Residues	4
Details	MOD_RES: N6-acetyllysine; alternate => ECO:0007744\|PubMed:19608861

Chain	Residue	Details
A	LYS212
B	LYS212
C	LYS212
D	LYS212

site_id	SWS_FT_FI7
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate => ECO:0007744\|PubMed:25755297

Chain	Residue	Details
A	LYS212
B	LYS212
C	LYS212
D	LYS212

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)