Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4M4S

Gamma subunit of the translation initiation factor 2 from Sulfolobus solfataricus in complex with GDP and formate ion mimic aIF2gammaGDPPi complex (a formate ion substitutes for Pi)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000049	molecular_function	tRNA binding
A	0000166	molecular_function	nucleotide binding
A	0001731	biological_process	formation of translation preinitiation complex
A	0003743	molecular_function	translation initiation factor activity
A	0003746	molecular_function	translation elongation factor activity
A	0003924	molecular_function	GTPase activity
A	0005515	molecular_function	protein binding
A	0005525	molecular_function	GTP binding
A	0006412	biological_process	translation
A	0006413	biological_process	translational initiation
A	0006414	biological_process	translational elongation
A	0016787	molecular_function	hydrolase activity
A	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE FMT A 501

Chain	Residue
A	MET45
A	THR46
A	PRO95
A	GLY96
A	HIS97
A	HOH901
A	HOH908

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FMT A 502

Chain	Residue
A	TRP327
A	HOH1013
A	SER268
A	TYR269

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE FMT A 503

Chain	Residue
A	ARG219
A	SER220
A	THR306
A	HOH621
A	HOH669

site_id	AC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE FMT A 504

Chain	Residue
A	SER314
A	VAL361
A	SER364
A	VAL386
A	ALA387
A	HOH643
A	HOH667

site_id	AC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FMT A 505

Chain	Residue
A	HIS37
A	SER38
A	ASN190
A	SER193
A	HOH607
A	HOH821

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FMT A 506

Chain	Residue
A	ALA342
A	LYS343
A	GLU344
A	HOH711

site_id	AC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE FMT A 507

Chain	Residue
A	ASN121
A	LYS150
A	ASP152
A	GDP530
A	HOH697

site_id	AC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE FMT A 508

Chain	Residue
A	LEU367
A	ARG384
A	HOH872

site_id	AC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FMT A 509

Chain	Residue
A	LYS254
A	ALA319
A	HOH729
A	HOH825

site_id	BC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FMT A 510

Chain	Residue
A	SER35
A	LYS36
A	GLU39
A	THR46
A	ASN224
A	HOH824

site_id	BC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FMT A 511

Chain	Residue
A	HIS37
A	LEU41
A	HOH716
A	HOH973
A	HOH974
A	HOH975

site_id	BC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE FMT A 512

Chain	Residue
A	ARG88
A	FMT513
A	HOH630
A	HOH915
A	HOH1025

site_id	BC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE FMT A 513

Chain	Residue
A	ASN55
A	ARG88
A	FMT512
A	HOH724
A	HOH785

site_id	BC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE FMT A 514

Chain	Residue
A	LYS64
A	TYR68
A	HOH847
A	HOH917
A	HOH1010

site_id	BC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE FMT A 515

Chain	Residue
A	LYS64
A	TYR68
A	HOH730

site_id	BC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE FMT A 516

Chain	Residue
A	LYS5
A	GLU285
A	PHE286

site_id	BC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE FMT A 517

Chain	Residue
A	LYS234
A	HOH800
A	HOH812

site_id	BC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FMT A 518

Chain	Residue
A	GLN400
A	GLN400
A	FMT519
A	HOH969

site_id	CC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE FMT A 519

Chain	Residue
A	FMT518
A	HOH762
A	HOH962

site_id	CC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FMT A 520

Chain	Residue
A	ARG130
A	SER161
A	ARG164
A	LYS343
A	HOH664
A	HOH714

site_id	CC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE FMT A 521

Chain	Residue
A	LYS42
A	TYR163
A	PRO179
A	ILE180
A	HOH801

site_id	CC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FMT A 522

Chain	Residue
A	TRP3
A	ASN55
A	PRO72
A	HOH630

site_id	CC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FMT A 523

Chain	Residue
A	LYS48
A	SER220
A	VAL223
A	ASN224
A	HOH846
A	HOH869

site_id	CC6
Number of Residues	2
Details	BINDING SITE FOR RESIDUE FMT A 524

Chain	Residue
A	ALA2
A	GLU82

site_id	CC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE FMT A 525

Chain	Residue
A	GLN27
A	ALA28
A	ALA185
A	LEU186
A	LYS188

site_id	CC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FMT A 526

Chain	Residue
A	VAL217
A	GLY313
A	SER363
A	HOH620
A	HOH946
A	HOH950

site_id	CC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FMT A 527

Chain	Residue
A	PHE286
A	LYS287
A	GLU288
A	HOH753

site_id	DC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE FMT A 528

Chain	Residue
A	LEU105
A	ILE139
A	SER360
A	VAL361
A	GLY362

site_id	DC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FMT A 529

Chain	Residue
A	VAL325
A	LEU326
A	TRP327
A	ASN328

site_id	DC3
Number of Residues	28
Details	BINDING SITE FOR RESIDUE GDP A 530

Chain	Residue
A	ASP19
A	HIS20
A	GLY21
A	LYS22
A	THR23
A	THR24
A	ASN149
A	LYS150
A	VAL151
A	ASP152
A	ASP152
A	VAL153
A	LYS156
A	SER184
A	ALA185
A	LEU186
A	FMT507
A	MG531
A	NA534
A	HOH618
A	HOH632
A	HOH901
A	HOH906
A	HOH907
A	HOH908
A	HOH909
A	HOH973
A	HOH991

site_id	DC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG A 531

Chain	Residue
A	THR23
A	GDP530
A	HOH901
A	HOH902
A	HOH906
A	HOH907

site_id	DC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG A 532

Chain	Residue
A	HIS187
A	HIS187
A	HIS187
A	HOH1037
A	HOH1037
A	HOH1037

site_id	DC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE NA A 533

Chain	Residue
A	GLU66
A	TYR68
A	SER76
A	HOH921
A	HOH922

site_id	DC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE NA A 534

Chain	Residue
A	VAL151
A	VAL154
A	GDP530
A	HOH614
A	HOH657
A	HOH973

site_id	DC8
Number of Residues	7
Details	BINDING SITE FOR RESIDUE NA A 535

Chain	Residue
A	MET111
A	HOH613
A	HOH675
A	HOH720
A	HOH779
A	HOH1004
A	HOH1017

site_id	DC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE NA A 536

Chain	Residue
A	THR54
A	THR70
A	HOH630
A	HOH638
A	HOH653
A	HOH683

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	199
Details	Domain: {"description":"tr-type G","evidences":[{"source":"HAMAP-Rule","id":"MF_00119","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	7
Details	Region: {"description":"G1","evidences":[{"source":"HAMAP-Rule","id":"MF_00119","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	4
Details	Region: {"description":"G2","evidences":[{"source":"HAMAP-Rule","id":"MF_00119","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	3
Details	Region: {"description":"G3","evidences":[{"source":"HAMAP-Rule","id":"MF_00119","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	3
Details	Region: {"description":"G4","evidences":[{"source":"HAMAP-Rule","id":"MF_00119","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	2
Details	Region: {"description":"G5","evidences":[{"source":"HAMAP-Rule","id":"MF_00119","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	10
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00119","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"25690901","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4RCY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4RD1","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00119","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"25690901","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4RD1","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00119","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"16407071","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25690901","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2AHO","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"Q8U082","evidenceCode":"ECO:0000250"},{"source":"HAMAP-Rule","id":"MF_00119","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)

4M4S

Gamma subunit of the translation initiation factor 2 from Sulfolobus solfataricus in complex with GDP and formate ion mimic aIF2gamma*GDP*Pi complex (a formate ion substitutes for Pi)

Gamma subunit of the translation initiation factor 2 from Sulfolobus solfataricus in complex with GDP and formate ion mimic aIF2gammaGDPPi complex (a formate ion substitutes for Pi)