4M30

Crystal structure of RNASE III complexed with double-stranded RNA AND AMP (TYPE II CLEAVAGE)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003723	molecular_function	RNA binding
A	0003725	molecular_function	double-stranded RNA binding
A	0004519	molecular_function	endonuclease activity
A	0004525	molecular_function	ribonuclease III activity
A	0005737	cellular_component	cytoplasm
A	0006364	biological_process	rRNA processing
A	0006396	biological_process	RNA processing
A	0006397	biological_process	mRNA processing
A	0008033	biological_process	tRNA processing
A	0010468	biological_process	regulation of gene expression
A	0042802	molecular_function	identical protein binding
A	0046872	molecular_function	metal ion binding
B	0003723	molecular_function	RNA binding
B	0003725	molecular_function	double-stranded RNA binding
B	0004519	molecular_function	endonuclease activity
B	0004525	molecular_function	ribonuclease III activity
B	0005737	cellular_component	cytoplasm
B	0006364	biological_process	rRNA processing
B	0006396	biological_process	RNA processing
B	0006397	biological_process	mRNA processing
B	0008033	biological_process	tRNA processing
B	0010468	biological_process	regulation of gene expression
B	0042802	molecular_function	identical protein binding
B	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MG A 501

Chain	Residue
A	ASP44
A	GLU110
A	MG502
A	HOH601
C	AMP103
D	U28
D	HOH201

---

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG A 502

Chain	Residue
A	MG501
A	HOH602
A	HOH603
C	AMP103
A	GLU40
A	GLU110

---

site_id	AC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MG B 301

Chain	Residue
B	ASP44
B	GLU110
B	MG302
B	HOH401
C	U28
C	HOH218
D	AMP104

---

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG B 302

Chain	Residue
B	GLU40
B	GLU110
B	MG301
B	HOH414
D	AMP104

---

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MPD B 303

Chain	Residue
A	ILE118
B	VAL56
B	GLN57
B	LYS62

---

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG C 101

Chain	Residue
A	HOH621
A	HOH622
A	HOH623
C	A2
C	HOH215
C	HOH216

---

site_id	AC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG C 102

Chain	Residue
C	A2
C	A3
C	HOH213
C	HOH214
C	HOH217

---

site_id	AC8
Number of Residues	16
Details	BINDING SITE FOR RESIDUE AMP C 103

Chain	Residue
A	GLU40
A	PHE41
A	ASP44
A	GLU110
A	MG501
A	MG502
A	HOH602
A	HOH603
A	HOH622
B	SER68
B	LYS71
C	A2
C	U26
C	A27
D	U28
D	HOH201

---

site_id	AC9
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MG D 101

Chain	Residue
A	GLU64
A	HOH624
B	HOH415
D	A2
D	HOH209
D	HOH211
D	HOH212

---

site_id	BC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG D 102

Chain	Residue
D	A2
D	A3
D	HOH204
D	HOH210

---

site_id	BC2
Number of Residues	1
Details	BINDING SITE FOR RESIDUE MG D 103

Chain	Residue
D	C23

---

site_id	BC3
Number of Residues	16
Details	BINDING SITE FOR RESIDUE AMP D 104

Chain	Residue
A	GLU64
A	SER68
A	LYS71
B	GLU40
B	PHE41
B	ASP44
B	GLU110
B	MG301
B	MG302
B	HOH414
C	U28
C	HOH218
D	A2
D	U26
D	A27
D	HOH211

---

Functional Information from PROSITE/UniProt

site_id	PS00517
Number of Residues	9
Details	RNASE_3_1 Ribonuclease III family signature. ETLEFLGDA

Chain	Residue	Details
A	GLU37-ALA45

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: ACT_SITE => ECO:0000255

Chain	Residue	Details
A	ASP44
B	ASP44

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	ACT_SITE: ACT_SITE => ECO:0000305

Chain	Residue	Details
A	GLU110
B	GLU110

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	BINDING: BINDING => ECO:0000269|PubMed:15016361, ECO:0000269|PubMed:18047582, ECO:0000305|PubMed:11738048, ECO:0007744|PDB:1JFZ, ECO:0007744|PDB:1RC5

Chain	Residue	Details
A	GLU40
A	ASP107
A	GLU110
B	GLU40
B	ASP107
B	GLU110

---