4M30

Crystal structure of RNASE III complexed with double-stranded RNA AND AMP (TYPE II CLEAVAGE)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-26
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	81.104, 81.104, 223.962
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.116 - 2.501
R-factor	0.2001
Rwork	0.198
R-free	0.26070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rc7
RMSD bond length	0.010
RMSD bond angle	1.052
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.590
High resolution limit [Å]	2.500	5.380	2.500
Rmerge	0.072	0.076	0.298
Number of reflections	28418
<I/σ(I)>	18.569	16.31	2.22
Completeness [%]	93.2	86	66.8
Redundancy	6.3	6.8	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	70% (v/v) MPD in 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K