4M2Z
Crystal structure of RNASE III complexed with double-stranded RNA and CMP (TYPE II CLEAVAGE)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003723 | molecular_function | RNA binding |
| A | 0003725 | molecular_function | double-stranded RNA binding |
| A | 0004518 | molecular_function | nuclease activity |
| A | 0004519 | molecular_function | endonuclease activity |
| A | 0004525 | molecular_function | ribonuclease III activity |
| A | 0004530 | molecular_function | deoxyribonuclease I activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0006364 | biological_process | rRNA processing |
| A | 0006396 | biological_process | RNA processing |
| A | 0006397 | biological_process | mRNA processing |
| A | 0008033 | biological_process | tRNA processing |
| A | 0010468 | biological_process | regulation of gene expression |
| A | 0016442 | cellular_component | RISC complex |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0030422 | biological_process | siRNA processing |
| A | 0031054 | biological_process | pre-miRNA processing |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0046872 | molecular_function | metal ion binding |
| B | 0003723 | molecular_function | RNA binding |
| B | 0003725 | molecular_function | double-stranded RNA binding |
| B | 0004518 | molecular_function | nuclease activity |
| B | 0004519 | molecular_function | endonuclease activity |
| B | 0004525 | molecular_function | ribonuclease III activity |
| B | 0004530 | molecular_function | deoxyribonuclease I activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0006364 | biological_process | rRNA processing |
| B | 0006396 | biological_process | RNA processing |
| B | 0006397 | biological_process | mRNA processing |
| B | 0008033 | biological_process | tRNA processing |
| B | 0010468 | biological_process | regulation of gene expression |
| B | 0016442 | cellular_component | RISC complex |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0030422 | biological_process | siRNA processing |
| B | 0031054 | biological_process | pre-miRNA processing |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG A 501 |
| Chain | Residue |
| A | ASP44 |
| A | GLU110 |
| A | HOH601 |
| C | C103 |
| D | G28 |
| D | HOH205 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG B 301 |
| Chain | Residue |
| C | G28 |
| D | C103 |
| D | HOH206 |
| B | ASP44 |
| B | GLU110 |
| B | HOH402 |
| site_id | AC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG C 101 |
| Chain | Residue |
| A | HOH606 |
| B | GLU64 |
| C | A2 |
| C | HOH204 |
| C | HOH205 |
| site_id | AC4 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE MG C 102 |
| Chain | Residue |
| C | A2 |
| C | A3 |
| site_id | AC5 |
| Number of Residues | 13 |
| Details | BINDING SITE FOR RESIDUE C C 103 |
| Chain | Residue |
| A | GLU40 |
| A | PHE41 |
| A | ASP44 |
| A | GLU110 |
| A | MG501 |
| B | GLU64 |
| B | SER68 |
| B | LYS71 |
| C | A2 |
| C | G26 |
| C | C27 |
| C | HOH204 |
| D | G28 |
| site_id | AC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG D 101 |
| Chain | Residue |
| A | GLU64 |
| B | HOH403 |
| D | A2 |
| D | HOH207 |
| D | HOH208 |
| site_id | AC7 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE MG D 102 |
| Chain | Residue |
| D | A2 |
| D | A3 |
| site_id | AC8 |
| Number of Residues | 14 |
| Details | BINDING SITE FOR RESIDUE C D 103 |
| Chain | Residue |
| A | GLU64 |
| A | SER68 |
| A | LYS71 |
| B | GLU40 |
| B | PHE41 |
| B | ASP44 |
| B | GLU110 |
| B | MG301 |
| C | G28 |
| D | A2 |
| D | G26 |
| D | C27 |
| D | HOH206 |
| D | HOH207 |
Functional Information from PROSITE/UniProt
| site_id | PS00517 |
| Number of Residues | 9 |
| Details | RNASE_3_1 Ribonuclease III family signature. ETLEFLGDA |
| Chain | Residue | Details |
| A | GLU37-ALA45 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: ACT_SITE => ECO:0000255 |
| Chain | Residue | Details |
| A | ASP44 | |
| B | ASP44 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | ACT_SITE: ACT_SITE => ECO:0000305 |
| Chain | Residue | Details |
| A | GLU110 | |
| B | GLU110 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15016361, ECO:0000269|PubMed:18047582, ECO:0000305|PubMed:11738048, ECO:0007744|PDB:1JFZ, ECO:0007744|PDB:1RC5 |
| Chain | Residue | Details |
| A | GLU40 | |
| A | ASP107 | |
| A | GLU110 | |
| B | GLU40 | |
| B | ASP107 | |
| B | GLU110 |
