4M2Z
Crystal structure of RNASE III complexed with double-stranded RNA and CMP (TYPE II CLEAVAGE)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-08 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 81.131, 81.131, 223.892 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.110 - 2.850 |
R-factor | 0.2244 |
Rwork | 0.221 |
R-free | 0.28870 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.003 |
RMSD bond angle | 0.698 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.950 |
High resolution limit [Å] | 2.850 | 6.120 | 2.850 |
Rmerge | 0.064 | 0.045 | 0.304 |
<I/σ(I)> | 23.1 | 25.97 | 1.86 |
Completeness [%] | 89.9 | 86.8 | 75.2 |
Redundancy | 4.6 | 5.5 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 70% (v/v) MPD in 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |