4L0Q

Crystal structure of S-nitrosoglutathione reductase from Arabidopsis thaliana, C370A/C373A double mutant

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004022	molecular_function	alcohol dehydrogenase (NAD+) activity
A	0004024	molecular_function	alcohol dehydrogenase (NAD+) activity, zinc-dependent
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006069	biological_process	obsolete ethanol oxidation
A	0008270	molecular_function	zinc ion binding
A	0016491	molecular_function	oxidoreductase activity
A	0046294	biological_process	formaldehyde catabolic process
A	0046872	molecular_function	metal ion binding
A	0051903	molecular_function	S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity
A	0106321	molecular_function	S-(hydroxymethyl)glutathione dehydrogenase (NADP+) activity
A	0106322	molecular_function	S-(hydroxymethyl)glutathione dehydrogenase (NAD+) activity
B	0004022	molecular_function	alcohol dehydrogenase (NAD+) activity
B	0004024	molecular_function	alcohol dehydrogenase (NAD+) activity, zinc-dependent
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006069	biological_process	obsolete ethanol oxidation
B	0008270	molecular_function	zinc ion binding
B	0016491	molecular_function	oxidoreductase activity
B	0046294	biological_process	formaldehyde catabolic process
B	0046872	molecular_function	metal ion binding
B	0051903	molecular_function	S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity
B	0106321	molecular_function	S-(hydroxymethyl)glutathione dehydrogenase (NADP+) activity
B	0106322	molecular_function	S-(hydroxymethyl)glutathione dehydrogenase (NAD+) activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 401

Chain	Residue
A	CYS99
A	CYS102
A	CYS105
A	CYS113

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 402

Chain	Residue
A	CYS47
A	HIS69
A	GLU70
A	CYS177

---

site_id	AC3
Number of Residues	30
Details	BINDING SITE FOR RESIDUE NAD A 403

Chain	Residue
A	TYR95
A	CYS177
A	THR181
A	GLY202
A	LEU203
A	GLY204
A	THR205
A	VAL206
A	ASP226
A	ILE227
A	LYS231
A	CYS271
A	ILE272
A	VAL277
A	VAL295
A	VAL297
A	THR320
A	ALA321
A	PHE322
A	ARG372
A	HOH507
A	HOH563
A	HOH575
A	HOH594
A	HOH600
A	HOH609
A	HOH611
A	HOH620
A	HOH671
A	HIS48

---

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PO4 A 404

Chain	Residue
A	LYS318
A	HOH582
A	HOH637
B	VAL316
B	LYS318

---

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE PO4 A 405

Chain	Residue
A	SER276
A	ARG279
A	SER305
A	HOH501

---

site_id	AC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE PO4 A 406

Chain	Residue
A	HIS249
A	ASP250
A	LYS251

---

site_id	AC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN B 401

Chain	Residue
B	CYS99
B	CYS102
B	CYS105
B	CYS113

---

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN B 402

Chain	Residue
B	CYS47
B	HIS69
B	GLU70
B	CYS177

---

site_id	AC9
Number of Residues	27
Details	BINDING SITE FOR RESIDUE NAD B 403

Chain	Residue
B	HIS48
B	TYR95
B	CYS177
B	THR181
B	GLY202
B	GLY204
B	THR205
B	VAL206
B	ASP226
B	ILE227
B	LYS231
B	CYS271
B	ILE272
B	VAL277
B	VAL295
B	GLY296
B	VAL297
B	THR320
B	ALA321
B	PHE322
B	ARG372
B	HOH514
B	HOH532
B	HOH535
B	HOH556
B	HOH561
B	HOH574

---

site_id	BC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE PO4 B 404

Chain	Residue
B	SER276
B	ARG279
B	HOH525
B	HOH690

---

site_id	BC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE PO4 B 405

Chain	Residue
B	HIS249
B	ASP250
B	LYS251

---

site_id	BC3
Number of Residues	9
Details	BINDING SITE FOR RESIDUE SO4 B 406

Chain	Residue
B	HOH517
B	HOH627
A	ARG307
A	PRO308
A	PHE309
B	ALA298
B	ALA299
B	SER300
B	GLY301

---

Functional Information from PROSITE/UniProt

site_id	PS00059
Number of Residues	15
Details	ADH_ZINC Zinc-containing alcohol dehydrogenases signature. GHEaAGIvesvGegV

Chain	Residue	Details
A	GLY68-VAL82

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	28
Details	BINDING: BINDING => ECO:0000269|Ref.13, ECO:0007744|PDB:3UKO, ECO:0007744|PDB:4GL4, ECO:0007744|PDB:4JJI, ECO:0007744|PDB:4L0Q

Chain	Residue	Details
A	CYS47
A	GLY202
A	ASP226
A	ILE272
A	VAL295
A	THR320
B	CYS47
B	HIS48
B	HIS69
B	GLU70
B	CYS99
A	HIS48
B	CYS102
B	CYS105
B	CYS113
B	CYS177
B	GLY202
B	ASP226
B	ILE272
B	VAL295
B	THR320
A	HIS69
A	GLU70
A	CYS99
A	CYS102
A	CYS105
A	CYS113
A	CYS177

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P06525

Chain	Residue	Details
A	THR49
B	THR49

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|Ref.13, ECO:0007744|PDB:3UKO, ECO:0007744|PDB:4JJI, ECO:0007744|PDB:4L0Q

Chain	Residue	Details
A	LYS231
A	ARG372
B	LYS231
B	ARG372

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: N-acetylalanine => ECO:0007744|PubMed:22223895

Chain	Residue	Details
A	ALA2
B	ALA2

---