4L0Q
Crystal structure of S-nitrosoglutathione reductase from Arabidopsis thaliana, C370A/C373A double mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-18 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.9795 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 92.731, 92.731, 173.558 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.650 - 1.950 |
R-factor | 0.20219 |
Rwork | 0.200 |
R-free | 0.25109 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3uko |
RMSD bond length | 0.018 |
RMSD bond angle | 2.037 |
Data reduction software | d*TREK |
Data scaling software | CrystalClear |
Phasing software | REFMAC |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.650 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.091 | 0.440 |
Number of reflections | 63603 | |
<I/σ(I)> | 15.6 | 4.4 |
Completeness [%] | 99.8 | 100 |
Redundancy | 12.1 | 12.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 2.1 M ammonium sulfate, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |