4KXF

Crystal structure of NLRC4 reveals its autoinhibition mechanism

Functional Information from GO Data

Chain	GOid	namespace	contents
B	0000166	molecular_function	nucleotide binding
B	0002218	biological_process	activation of innate immune response
B	0002376	biological_process	immune system process
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0006915	biological_process	apoptotic process
B	0006954	biological_process	inflammatory response
B	0010954	biological_process	positive regulation of protein processing
B	0016045	biological_process	detection of bacterium
B	0032731	biological_process	positive regulation of interleukin-1 beta production
B	0042742	biological_process	defense response to bacterium
B	0042802	molecular_function	identical protein binding
B	0042803	molecular_function	protein homodimerization activity
B	0042981	biological_process	regulation of apoptotic process
B	0043065	biological_process	positive regulation of apoptotic process
B	0045087	biological_process	innate immune response
B	0051260	biological_process	protein homooligomerization
B	0061133	molecular_function	endopeptidase activator activity
B	0061702	cellular_component	canonical inflammasome complex
B	0070269	biological_process	pyroptotic inflammatory response
B	0072557	cellular_component	IPAF inflammasome complex
B	0089720	molecular_function	caspase binding
D	0000166	molecular_function	nucleotide binding
D	0002218	biological_process	activation of innate immune response
D	0002376	biological_process	immune system process
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0006915	biological_process	apoptotic process
D	0006954	biological_process	inflammatory response
D	0010954	biological_process	positive regulation of protein processing
D	0016045	biological_process	detection of bacterium
D	0032731	biological_process	positive regulation of interleukin-1 beta production
D	0042742	biological_process	defense response to bacterium
D	0042802	molecular_function	identical protein binding
D	0042803	molecular_function	protein homodimerization activity
D	0042981	biological_process	regulation of apoptotic process
D	0043065	biological_process	positive regulation of apoptotic process
D	0045087	biological_process	innate immune response
D	0051260	biological_process	protein homooligomerization
D	0061133	molecular_function	endopeptidase activator activity
D	0061702	cellular_component	canonical inflammasome complex
D	0070269	biological_process	pyroptotic inflammatory response
D	0072557	cellular_component	IPAF inflammasome complex
D	0089720	molecular_function	caspase binding
F	0000166	molecular_function	nucleotide binding
F	0002218	biological_process	activation of innate immune response
F	0002376	biological_process	immune system process
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005829	cellular_component	cytosol
F	0005886	cellular_component	plasma membrane
F	0006915	biological_process	apoptotic process
F	0006954	biological_process	inflammatory response
F	0010954	biological_process	positive regulation of protein processing
F	0016045	biological_process	detection of bacterium
F	0032731	biological_process	positive regulation of interleukin-1 beta production
F	0042742	biological_process	defense response to bacterium
F	0042802	molecular_function	identical protein binding
F	0042803	molecular_function	protein homodimerization activity
F	0042981	biological_process	regulation of apoptotic process
F	0043065	biological_process	positive regulation of apoptotic process
F	0045087	biological_process	innate immune response
F	0051260	biological_process	protein homooligomerization
F	0061133	molecular_function	endopeptidase activator activity
F	0061702	cellular_component	canonical inflammasome complex
F	0070269	biological_process	pyroptotic inflammatory response
F	0072557	cellular_component	IPAF inflammasome complex
F	0089720	molecular_function	caspase binding
H	0000166	molecular_function	nucleotide binding
H	0002218	biological_process	activation of innate immune response
H	0002376	biological_process	immune system process
H	0005515	molecular_function	protein binding
H	0005524	molecular_function	ATP binding
H	0005829	cellular_component	cytosol
H	0005886	cellular_component	plasma membrane
H	0006915	biological_process	apoptotic process
H	0006954	biological_process	inflammatory response
H	0010954	biological_process	positive regulation of protein processing
H	0016045	biological_process	detection of bacterium
H	0032731	biological_process	positive regulation of interleukin-1 beta production
H	0042742	biological_process	defense response to bacterium
H	0042802	molecular_function	identical protein binding
H	0042803	molecular_function	protein homodimerization activity
H	0042981	biological_process	regulation of apoptotic process
H	0043065	biological_process	positive regulation of apoptotic process
H	0045087	biological_process	innate immune response
H	0051260	biological_process	protein homooligomerization
H	0061133	molecular_function	endopeptidase activator activity
H	0061702	cellular_component	canonical inflammasome complex
H	0070269	biological_process	pyroptotic inflammatory response
H	0072557	cellular_component	IPAF inflammasome complex
H	0089720	molecular_function	caspase binding
K	0000166	molecular_function	nucleotide binding
K	0002218	biological_process	activation of innate immune response
K	0002376	biological_process	immune system process
K	0005515	molecular_function	protein binding
K	0005524	molecular_function	ATP binding
K	0005829	cellular_component	cytosol
K	0005886	cellular_component	plasma membrane
K	0006915	biological_process	apoptotic process
K	0006954	biological_process	inflammatory response
K	0010954	biological_process	positive regulation of protein processing
K	0016045	biological_process	detection of bacterium
K	0032731	biological_process	positive regulation of interleukin-1 beta production
K	0042742	biological_process	defense response to bacterium
K	0042802	molecular_function	identical protein binding
K	0042803	molecular_function	protein homodimerization activity
K	0042981	biological_process	regulation of apoptotic process
K	0043065	biological_process	positive regulation of apoptotic process
K	0045087	biological_process	innate immune response
K	0051260	biological_process	protein homooligomerization
K	0061133	molecular_function	endopeptidase activator activity
K	0061702	cellular_component	canonical inflammasome complex
K	0070269	biological_process	pyroptotic inflammatory response
K	0072557	cellular_component	IPAF inflammasome complex
K	0089720	molecular_function	caspase binding
L	0000166	molecular_function	nucleotide binding
L	0002218	biological_process	activation of innate immune response
L	0002376	biological_process	immune system process
L	0005515	molecular_function	protein binding
L	0005524	molecular_function	ATP binding
L	0005829	cellular_component	cytosol
L	0005886	cellular_component	plasma membrane
L	0006915	biological_process	apoptotic process
L	0006954	biological_process	inflammatory response
L	0010954	biological_process	positive regulation of protein processing
L	0016045	biological_process	detection of bacterium
L	0032731	biological_process	positive regulation of interleukin-1 beta production
L	0042742	biological_process	defense response to bacterium
L	0042802	molecular_function	identical protein binding
L	0042803	molecular_function	protein homodimerization activity
L	0042981	biological_process	regulation of apoptotic process
L	0043065	biological_process	positive regulation of apoptotic process
L	0045087	biological_process	innate immune response
L	0051260	biological_process	protein homooligomerization
L	0061133	molecular_function	endopeptidase activator activity
L	0061702	cellular_component	canonical inflammasome complex
L	0070269	biological_process	pyroptotic inflammatory response
L	0072557	cellular_component	IPAF inflammasome complex
L	0089720	molecular_function	caspase binding
N	0000166	molecular_function	nucleotide binding
N	0002218	biological_process	activation of innate immune response
N	0002376	biological_process	immune system process
N	0005515	molecular_function	protein binding
N	0005524	molecular_function	ATP binding
N	0005829	cellular_component	cytosol
N	0005886	cellular_component	plasma membrane
N	0006915	biological_process	apoptotic process
N	0006954	biological_process	inflammatory response
N	0010954	biological_process	positive regulation of protein processing
N	0016045	biological_process	detection of bacterium
N	0032731	biological_process	positive regulation of interleukin-1 beta production
N	0042742	biological_process	defense response to bacterium
N	0042802	molecular_function	identical protein binding
N	0042803	molecular_function	protein homodimerization activity
N	0042981	biological_process	regulation of apoptotic process
N	0043065	biological_process	positive regulation of apoptotic process
N	0045087	biological_process	innate immune response
N	0051260	biological_process	protein homooligomerization
N	0061133	molecular_function	endopeptidase activator activity
N	0061702	cellular_component	canonical inflammasome complex
N	0070269	biological_process	pyroptotic inflammatory response
N	0072557	cellular_component	IPAF inflammasome complex
N	0089720	molecular_function	caspase binding
P	0000166	molecular_function	nucleotide binding
P	0002218	biological_process	activation of innate immune response
P	0002376	biological_process	immune system process
P	0005515	molecular_function	protein binding
P	0005524	molecular_function	ATP binding
P	0005829	cellular_component	cytosol
P	0005886	cellular_component	plasma membrane
P	0006915	biological_process	apoptotic process
P	0006954	biological_process	inflammatory response
P	0010954	biological_process	positive regulation of protein processing
P	0016045	biological_process	detection of bacterium
P	0032731	biological_process	positive regulation of interleukin-1 beta production
P	0042742	biological_process	defense response to bacterium
P	0042802	molecular_function	identical protein binding
P	0042803	molecular_function	protein homodimerization activity
P	0042981	biological_process	regulation of apoptotic process
P	0043065	biological_process	positive regulation of apoptotic process
P	0045087	biological_process	innate immune response
P	0051260	biological_process	protein homooligomerization
P	0061133	molecular_function	endopeptidase activator activity
P	0061702	cellular_component	canonical inflammasome complex
P	0070269	biological_process	pyroptotic inflammatory response
P	0072557	cellular_component	IPAF inflammasome complex
P	0089720	molecular_function	caspase binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	19
Details	BINDING SITE FOR RESIDUE ADP K 1101

Chain	Residue
K	PHE117
K	GLY174
K	LYS175
K	SER176
K	THR177
K	MET300
K	LEU308
K	PRO338
K	LEU339
K	VAL342
K	HIS443
K	PRO119
K	PHE128
K	THR133
K	PHE134
K	THR135
K	SER171
K	GLY172
K	LYS173

---

site_id	AC2
Number of Residues	16
Details	BINDING SITE FOR RESIDUE ADP B 1101

Chain	Residue
B	PRO119
B	PHE128
B	PHE134
B	THR135
B	GLY172
B	LYS173
B	GLY174
B	LYS175
B	SER176
B	THR177
B	MET300
B	LEU308
B	PRO338
B	LEU339
B	VAL342
B	HIS443

---

site_id	AC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 B 1102

Chain	Residue
B	HIS254
B	HIS404

---

site_id	AC4
Number of Residues	16
Details	BINDING SITE FOR RESIDUE ADP D 1101

Chain	Residue
D	PRO119
D	PHE128
D	THR133
D	PHE134
D	THR135
D	GLY172
D	LYS173
D	GLY174
D	LYS175
D	SER176
D	THR177
D	MET300
D	LEU308
D	PRO338
D	LEU339
D	HIS443

---

site_id	AC5
Number of Residues	14
Details	BINDING SITE FOR RESIDUE ADP F 1101

Chain	Residue
F	PRO119
F	PHE128
F	PHE134
F	THR135
F	GLY172
F	LYS173
F	GLY174
F	LYS175
F	SER176
F	THR177
F	PRO338
F	LEU339
F	VAL342
F	HIS443

---

site_id	AC6
Number of Residues	17
Details	BINDING SITE FOR RESIDUE ADP H 1101

Chain	Residue
H	PRO119
H	PHE128
H	PHE134
H	THR135
H	GLU170
H	GLY172
H	LYS173
H	GLY174
H	LYS175
H	SER176
H	THR177
H	MET300
H	LEU308
H	PRO338
H	LEU339
H	VAL342
H	HIS443

---

site_id	AC7
Number of Residues	17
Details	BINDING SITE FOR RESIDUE ADP L 1101

Chain	Residue
L	LEU339
L	HIS443
L	PHE117
L	PRO119
L	PHE128
L	PHE134
L	THR135
L	SER171
L	GLY172
L	LYS173
L	GLY174
L	LYS175
L	SER176
L	THR177
L	MET300
L	LEU308
L	PRO338

---

site_id	AC8
Number of Residues	16
Details	BINDING SITE FOR RESIDUE ADP N 1101

Chain	Residue
N	PRO119
N	PHE128
N	PHE134
N	THR135
N	SER171
N	GLY172
N	LYS173
N	GLY174
N	LYS175
N	SER176
N	THR177
N	MET300
N	LEU308
N	PRO338
N	LEU339
N	HIS443

---

site_id	AC9
Number of Residues	16
Details	BINDING SITE FOR RESIDUE ADP P 1101

Chain	Residue
P	PHE117
P	PRO119
P	PHE128
P	PHE134
P	THR135
P	GLY172
P	LYS173
P	GLY174
P	LYS175
P	SER176
P	THR177
P	MET300
P	LEU308
P	PRO338
P	LEU339
P	HIS443

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	160
Details	Repeat: {"description":"LRR 1","evidences":[{"source":"PubMed","id":"23765277","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	184
Details	Repeat: {"description":"LRR 2","evidences":[{"source":"PubMed","id":"23765277","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	184
Details	Repeat: {"description":"LRR 3","evidences":[{"source":"PubMed","id":"23765277","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	184
Details	Repeat: {"description":"LRR 4","evidences":[{"source":"PubMed","id":"23765277","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	200
Details	Repeat: {"description":"LRR 5","evidences":[{"source":"PubMed","id":"23765277","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	184
Details	Repeat: {"description":"LRR 6","evidences":[{"source":"PubMed","id":"23765277","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	176
Details	Repeat: {"description":"LRR 7","evidences":[{"source":"PubMed","id":"23765277","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	192
Details	Repeat: {"description":"LRR 8","evidences":[{"source":"PubMed","id":"23765277","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	176
Details	Repeat: {"description":"LRR 9","evidences":[{"source":"PubMed","id":"23765277","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	216
Details	Repeat: {"description":"LRR 10","evidences":[{"source":"PubMed","id":"23765277","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	160
Details	Repeat: {"description":"LRR 11","evidences":[{"source":"PubMed","id":"23765277","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	1624
Details	Region: {"description":"Nucleotide-binding domain (NBD)"}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	56
Details	Binding site: {"evidences":[{"source":"PubMed","id":"23765277","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4KXF","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	7
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"22885697","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"23765277","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	939
Details	Domain: {"description":"NACHT","evidences":[{"source":"PROSITE-ProRule","id":"PRU00136","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	321
Details	Region: {"description":"Winged-helix domain (WHD)"}

Chain

Residue

Details

---