Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4KXF

Crystal structure of NLRC4 reveals its autoinhibition mechanism

Functional Information from GO Data

Chain	GOid	namespace	contents
B	0002218	biological_process	activation of innate immune response
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006915	biological_process	apoptotic process
B	0006954	biological_process	inflammatory response
B	0016045	biological_process	detection of bacterium
B	0032731	biological_process	positive regulation of interleukin-1 beta production
B	0042742	biological_process	defense response to bacterium
B	0042802	molecular_function	identical protein binding
B	0042803	molecular_function	protein homodimerization activity
B	0042981	biological_process	regulation of apoptotic process
B	0045087	biological_process	innate immune response
B	0051260	biological_process	protein homooligomerization
B	0061702	cellular_component	canonical inflammasome complex
B	0070269	biological_process	pyroptotic inflammatory response
B	0072557	cellular_component	IPAF inflammasome complex
D	0002218	biological_process	activation of innate immune response
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006915	biological_process	apoptotic process
D	0006954	biological_process	inflammatory response
D	0016045	biological_process	detection of bacterium
D	0032731	biological_process	positive regulation of interleukin-1 beta production
D	0042742	biological_process	defense response to bacterium
D	0042802	molecular_function	identical protein binding
D	0042803	molecular_function	protein homodimerization activity
D	0042981	biological_process	regulation of apoptotic process
D	0045087	biological_process	innate immune response
D	0051260	biological_process	protein homooligomerization
D	0061702	cellular_component	canonical inflammasome complex
D	0070269	biological_process	pyroptotic inflammatory response
D	0072557	cellular_component	IPAF inflammasome complex
F	0002218	biological_process	activation of innate immune response
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006915	biological_process	apoptotic process
F	0006954	biological_process	inflammatory response
F	0016045	biological_process	detection of bacterium
F	0032731	biological_process	positive regulation of interleukin-1 beta production
F	0042742	biological_process	defense response to bacterium
F	0042802	molecular_function	identical protein binding
F	0042803	molecular_function	protein homodimerization activity
F	0042981	biological_process	regulation of apoptotic process
F	0045087	biological_process	innate immune response
F	0051260	biological_process	protein homooligomerization
F	0061702	cellular_component	canonical inflammasome complex
F	0070269	biological_process	pyroptotic inflammatory response
F	0072557	cellular_component	IPAF inflammasome complex
H	0002218	biological_process	activation of innate immune response
H	0005515	molecular_function	protein binding
H	0005524	molecular_function	ATP binding
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006915	biological_process	apoptotic process
H	0006954	biological_process	inflammatory response
H	0016045	biological_process	detection of bacterium
H	0032731	biological_process	positive regulation of interleukin-1 beta production
H	0042742	biological_process	defense response to bacterium
H	0042802	molecular_function	identical protein binding
H	0042803	molecular_function	protein homodimerization activity
H	0042981	biological_process	regulation of apoptotic process
H	0045087	biological_process	innate immune response
H	0051260	biological_process	protein homooligomerization
H	0061702	cellular_component	canonical inflammasome complex
H	0070269	biological_process	pyroptotic inflammatory response
H	0072557	cellular_component	IPAF inflammasome complex
K	0002218	biological_process	activation of innate immune response
K	0005515	molecular_function	protein binding
K	0005524	molecular_function	ATP binding
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0006915	biological_process	apoptotic process
K	0006954	biological_process	inflammatory response
K	0016045	biological_process	detection of bacterium
K	0032731	biological_process	positive regulation of interleukin-1 beta production
K	0042742	biological_process	defense response to bacterium
K	0042802	molecular_function	identical protein binding
K	0042803	molecular_function	protein homodimerization activity
K	0042981	biological_process	regulation of apoptotic process
K	0045087	biological_process	innate immune response
K	0051260	biological_process	protein homooligomerization
K	0061702	cellular_component	canonical inflammasome complex
K	0070269	biological_process	pyroptotic inflammatory response
K	0072557	cellular_component	IPAF inflammasome complex
L	0002218	biological_process	activation of innate immune response
L	0005515	molecular_function	protein binding
L	0005524	molecular_function	ATP binding
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0006915	biological_process	apoptotic process
L	0006954	biological_process	inflammatory response
L	0016045	biological_process	detection of bacterium
L	0032731	biological_process	positive regulation of interleukin-1 beta production
L	0042742	biological_process	defense response to bacterium
L	0042802	molecular_function	identical protein binding
L	0042803	molecular_function	protein homodimerization activity
L	0042981	biological_process	regulation of apoptotic process
L	0045087	biological_process	innate immune response
L	0051260	biological_process	protein homooligomerization
L	0061702	cellular_component	canonical inflammasome complex
L	0070269	biological_process	pyroptotic inflammatory response
L	0072557	cellular_component	IPAF inflammasome complex
N	0002218	biological_process	activation of innate immune response
N	0005515	molecular_function	protein binding
N	0005524	molecular_function	ATP binding
N	0005737	cellular_component	cytoplasm
N	0005829	cellular_component	cytosol
N	0006915	biological_process	apoptotic process
N	0006954	biological_process	inflammatory response
N	0016045	biological_process	detection of bacterium
N	0032731	biological_process	positive regulation of interleukin-1 beta production
N	0042742	biological_process	defense response to bacterium
N	0042802	molecular_function	identical protein binding
N	0042803	molecular_function	protein homodimerization activity
N	0042981	biological_process	regulation of apoptotic process
N	0045087	biological_process	innate immune response
N	0051260	biological_process	protein homooligomerization
N	0061702	cellular_component	canonical inflammasome complex
N	0070269	biological_process	pyroptotic inflammatory response
N	0072557	cellular_component	IPAF inflammasome complex
P	0002218	biological_process	activation of innate immune response
P	0005515	molecular_function	protein binding
P	0005524	molecular_function	ATP binding
P	0005737	cellular_component	cytoplasm
P	0005829	cellular_component	cytosol
P	0006915	biological_process	apoptotic process
P	0006954	biological_process	inflammatory response
P	0016045	biological_process	detection of bacterium
P	0032731	biological_process	positive regulation of interleukin-1 beta production
P	0042742	biological_process	defense response to bacterium
P	0042802	molecular_function	identical protein binding
P	0042803	molecular_function	protein homodimerization activity
P	0042981	biological_process	regulation of apoptotic process
P	0045087	biological_process	innate immune response
P	0051260	biological_process	protein homooligomerization
P	0061702	cellular_component	canonical inflammasome complex
P	0070269	biological_process	pyroptotic inflammatory response
P	0072557	cellular_component	IPAF inflammasome complex

Functional Information from PDB Data

site_id	AC1
Number of Residues	19
Details	BINDING SITE FOR RESIDUE ADP K 1101

Chain	Residue
K	PHE117
K	GLY174
K	LYS175
K	SER176
K	THR177
K	MET300
K	LEU308
K	PRO338
K	LEU339
K	VAL342
K	HIS443
K	PRO119
K	PHE128
K	THR133
K	PHE134
K	THR135
K	SER171
K	GLY172
K	LYS173

site_id	AC2
Number of Residues	16
Details	BINDING SITE FOR RESIDUE ADP B 1101

Chain	Residue
B	PRO119
B	PHE128
B	PHE134
B	THR135
B	GLY172
B	LYS173
B	GLY174
B	LYS175
B	SER176
B	THR177
B	MET300
B	LEU308
B	PRO338
B	LEU339
B	VAL342
B	HIS443

site_id	AC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 B 1102

Chain	Residue
B	HIS254
B	HIS404

site_id	AC4
Number of Residues	16
Details	BINDING SITE FOR RESIDUE ADP D 1101

Chain	Residue
D	PRO119
D	PHE128
D	THR133
D	PHE134
D	THR135
D	GLY172
D	LYS173
D	GLY174
D	LYS175
D	SER176
D	THR177
D	MET300
D	LEU308
D	PRO338
D	LEU339
D	HIS443

site_id	AC5
Number of Residues	14
Details	BINDING SITE FOR RESIDUE ADP F 1101

Chain	Residue
F	PRO119
F	PHE128
F	PHE134
F	THR135
F	GLY172
F	LYS173
F	GLY174
F	LYS175
F	SER176
F	THR177
F	PRO338
F	LEU339
F	VAL342
F	HIS443

site_id	AC6
Number of Residues	17
Details	BINDING SITE FOR RESIDUE ADP H 1101

Chain	Residue
H	PRO119
H	PHE128
H	PHE134
H	THR135
H	GLU170
H	GLY172
H	LYS173
H	GLY174
H	LYS175
H	SER176
H	THR177
H	MET300
H	LEU308
H	PRO338
H	LEU339
H	VAL342
H	HIS443

site_id	AC7
Number of Residues	17
Details	BINDING SITE FOR RESIDUE ADP L 1101

Chain	Residue
L	LEU339
L	HIS443
L	PHE117
L	PRO119
L	PHE128
L	PHE134
L	THR135
L	SER171
L	GLY172
L	LYS173
L	GLY174
L	LYS175
L	SER176
L	THR177
L	MET300
L	LEU308
L	PRO338

site_id	AC8
Number of Residues	16
Details	BINDING SITE FOR RESIDUE ADP N 1101

Chain	Residue
N	PRO119
N	PHE128
N	PHE134
N	THR135
N	SER171
N	GLY172
N	LYS173
N	GLY174
N	LYS175
N	SER176
N	THR177
N	MET300
N	LEU308
N	PRO338
N	LEU339
N	HIS443

site_id	AC9
Number of Residues	16
Details	BINDING SITE FOR RESIDUE ADP P 1101

Chain	Residue
P	PHE117
P	PRO119
P	PHE128
P	PHE134
P	THR135
P	GLY172
P	LYS173
P	GLY174
P	LYS175
P	SER176
P	THR177
P	MET300
P	LEU308
P	PRO338
P	LEU339
P	HIS443

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	24
Details	BINDING: BINDING => ECO:0000269\|PubMed:23765277, ECO:0007744\|PDB:4KXF

Chain	Residue	Details
K	THR135
F	THR135
F	GLY172
F	HIS443
H	THR135
H	GLY172
H	HIS443
L	THR135
L	GLY172
L	HIS443
N	THR135
K	GLY172
N	GLY172
N	HIS443
P	THR135
P	GLY172
P	HIS443
K	HIS443
B	THR135
B	GLY172
B	HIS443
D	THR135
D	GLY172
D	HIS443

site_id	SWS_FT_FI2
Number of Residues	8
Details	MOD_RES: Phosphoserine => ECO:0000269\|PubMed:22885697, ECO:0000269\|PubMed:23765277

Chain	Residue	Details
K	SEP533
B	SEP533
D	SEP533
F	SEP533
H	SEP533
L	SEP533
N	SEP533
P	SEP533

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)