4KJD

RatIntestinal AP expressed in E. coli

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0002020	molecular_function	protease binding
A	0004035	molecular_function	alkaline phosphatase activity
A	0005515	molecular_function	protein binding
A	0005886	cellular_component	plasma membrane
A	0008270	molecular_function	zinc ion binding
A	0009897	cellular_component	external side of plasma membrane
A	0016311	biological_process	dephosphorylation
A	0016787	molecular_function	hydrolase activity
A	0016791	molecular_function	phosphatase activity
A	0042803	molecular_function	protein homodimerization activity
A	0046872	molecular_function	metal ion binding
A	0071773	biological_process	cellular response to BMP stimulus
A	0098552	cellular_component	side of membrane
B	0000287	molecular_function	magnesium ion binding
B	0002020	molecular_function	protease binding
B	0004035	molecular_function	alkaline phosphatase activity
B	0005515	molecular_function	protein binding
B	0005886	cellular_component	plasma membrane
B	0008270	molecular_function	zinc ion binding
B	0009897	cellular_component	external side of plasma membrane
B	0016311	biological_process	dephosphorylation
B	0016787	molecular_function	hydrolase activity
B	0016791	molecular_function	phosphatase activity
B	0042803	molecular_function	protein homodimerization activity
B	0046872	molecular_function	metal ion binding
B	0071773	biological_process	cellular response to BMP stimulus
B	0098552	cellular_component	side of membrane

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG A 501

Chain	Residue
A	SER155
A	GLU311
A	HOH734
A	HOH735
A	HOH736
A	HOH737

---

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG A 502

Chain	Residue
A	ASP285
A	HOH634
A	GLU216
A	PHE269
A	GLU270

---

site_id	AC3
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL A 503

Chain	Residue
A	LYS34
A	ASN35
A	SER300
A	THR345
A	ASN346
A	ASP349
A	THR350
A	HOH651

---

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG B 501

Chain	Residue
B	SER155
B	GLU311
B	HOH704
B	HOH737
B	HOH738
B	HOH739

---

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG B 502

Chain	Residue
B	GLU216
B	PHE269
B	GLU270
B	ASP285
B	HOH609

---

site_id	AC6
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL B 503

Chain	Residue
B	LYS34
B	ASN35
B	SER300
B	ASN346
B	ASP349
B	THR350
B	HOH631

---

Functional Information from PROSITE/UniProt

site_id	PS00123
Number of Residues	9
Details	ALKALINE_PHOSPHATASE Alkaline phosphatase active site. VpDSAGTAT

Chain	Residue	Details
A	VAL89-THR97

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Phosphoserine intermediate => ECO:0000255|PROSITE-ProRule:PRU10042, ECO:0000269|PubMed:24076154

Chain	Residue	Details
A	SEP92
B	SEP92

---

site_id	SWS_FT_FI2
Number of Residues	18
Details	BINDING: BINDING => ECO:0000269|PubMed:24076154

Chain	Residue	Details
A	ASP42
B	ASP42
B	SEP92
B	SER155
B	GLU311
B	ASP316
B	HIS320
B	ASP357
B	HIS358
B	HIS432
A	SEP92
A	SER155
A	GLU311
A	ASP316
A	HIS320
A	ASP357
A	HIS358
A	HIS432

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P05186

Chain	Residue	Details
A	GLU216
A	PHE269
A	GLU270
A	ASP285
B	GLU216
B	PHE269
B	GLU270
B	ASP285

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255

Chain	Residue	Details
A	ASN122
B	ASN122

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:24076154

Chain	Residue	Details
A	ASN281
A	ASN408
B	ASN281
B	ASN408

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