4KJD
RatIntestinal AP expressed in E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.22 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.321, 75.726, 170.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.680 - 2.210 |
| R-factor | 0.1691 |
| Rwork | 0.167 |
| R-free | 0.20340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ew2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (BUSTER 2.11.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 4.740 | 2.200 |
| Rmerge | 0.096 | 0.057 | 0.592 |
| Number of reflections | 42064 | ||
| <I/σ(I)> | 7.2 | ||
| Completeness [%] | 99.9 | 99.6 | 100 |
| Redundancy | 6 | 5.7 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 0.1 M NH4Cl, 0.1 M MES 6.5, 20 % PEG MME 2000, Vapor diffusion, temperature 293K |
