4JS4

Crystal structure of E. coli Exonuclease I in complex with a dA16 oligonucleotide

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000175	molecular_function	3'-5'-RNA exonuclease activity
A	0000287	molecular_function	magnesium ion binding
A	0003676	molecular_function	nucleic acid binding
A	0003677	molecular_function	DNA binding
A	0003697	molecular_function	single-stranded DNA binding
A	0004518	molecular_function	nuclease activity
A	0004527	molecular_function	exonuclease activity
A	0004529	molecular_function	DNA exonuclease activity
A	0005515	molecular_function	protein binding
A	0006259	biological_process	DNA metabolic process
A	0006274	biological_process	DNA replication termination
A	0006281	biological_process	DNA repair
A	0006308	biological_process	DNA catabolic process
A	0006974	biological_process	DNA damage response
A	0008310	molecular_function	single-stranded DNA 3'-5' DNA exonuclease activity
A	0016787	molecular_function	hydrolase activity
A	0046872	molecular_function	metal ion binding
A	0051575	molecular_function	5'-deoxyribose-5-phosphate lyase activity
B	0000175	molecular_function	3'-5'-RNA exonuclease activity
B	0000287	molecular_function	magnesium ion binding
B	0003676	molecular_function	nucleic acid binding
B	0003677	molecular_function	DNA binding
B	0003697	molecular_function	single-stranded DNA binding
B	0004518	molecular_function	nuclease activity
B	0004527	molecular_function	exonuclease activity
B	0004529	molecular_function	DNA exonuclease activity
B	0005515	molecular_function	protein binding
B	0006259	biological_process	DNA metabolic process
B	0006274	biological_process	DNA replication termination
B	0006281	biological_process	DNA repair
B	0006308	biological_process	DNA catabolic process
B	0006974	biological_process	DNA damage response
B	0008310	molecular_function	single-stranded DNA 3'-5' DNA exonuclease activity
B	0016787	molecular_function	hydrolase activity
B	0046872	molecular_function	metal ion binding
B	0051575	molecular_function	5'-deoxyribose-5-phosphate lyase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 A 501

Chain	Residue
A	HIS215
A	MET218
A	ARG256
B	LYS214
B	HIS215
B	MET218
B	ARG256

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 502

Chain	Residue
A	LYS305
A	SO4508
A	ARG106
A	HIS302

---

site_id	AC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 503

Chain	Residue
A	ARG148
A	ARG203
A	ALA393

---

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 504

Chain	Residue
A	TYR207
A	THR210
A	HIS211
A	ASN390

---

site_id	AC5
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 A 505

Chain	Residue
A	PHE209
A	ARG212

---

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 506

Chain	Residue
A	LYS198
A	PHE205
A	ASP206
A	PHE209

---

site_id	AC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 507

Chain	Residue
A	PHE415
A	PRO416
A	GLY417

---

site_id	AC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 A 508

Chain	Residue
A	ARG106
A	ASP139
A	ARG142
A	SER233
A	GLY234
A	SO4502

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	179
Details	Domain: {"description":"Exonuclease","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	153
Details	Domain: {"description":"ExoI SH3-like","evidences":[{"source":"PROSITE-ProRule","id":"PRU01120","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	117
Details	Domain: {"description":"ExoI C-terminal","evidences":[{"source":"PROSITE-ProRule","id":"PRU01121","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11101894","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18219121","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18591666","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20018747","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"23609540","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"PubMed","id":"18219121","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	20
Details	Site: {"description":"Interaction with single-stranded DNA","evidences":[{"source":"PubMed","id":"23609540","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	6
Details	Site: {"description":"Important for interaction with ssb","evidences":[{"source":"PubMed","id":"18591666","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	6
Details	Site: {"description":"Interaction with single-stranded DNA","evidences":[{"source":"PubMed","id":"23609540","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4HCC","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	Site: {"description":"Important for activity","evidences":[{"source":"PubMed","id":"23609540","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	Site: {"description":"Important for interaction with ssb","evidences":[{"source":"PubMed","id":"20018747","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---