4JS4
Crystal structure of E. coli Exonuclease I in complex with a dA16 oligonucleotide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-16 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.97931 |
Spacegroup name | P 43 |
Unit cell lengths | 91.413, 91.413, 162.518 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.600 - 3.100 |
R-factor | 0.22655 |
Rwork | 0.225 |
R-free | 0.25665 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fxx |
RMSD bond length | 0.004 |
RMSD bond angle | 0.714 |
Data reduction software | MOSFLM |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.600 | 3.270 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.066 | 0.813 |
Number of reflections | 22917 | |
<I/σ(I)> | 22.4 | 2.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.6 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 5% 2-propanol, 25% glycerol, 1.2M ammonium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |