4JS4
Crystal structure of E. coli Exonuclease I in complex with a dA16 oligonucleotide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-16 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 43 |
| Unit cell lengths | 91.413, 91.413, 162.518 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.600 - 3.100 |
| R-factor | 0.22655 |
| Rwork | 0.225 |
| R-free | 0.25665 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fxx |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.714 |
| Data reduction software | MOSFLM |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.600 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.066 | 0.813 |
| Number of reflections | 22917 | |
| <I/σ(I)> | 22.4 | 2.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.6 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 5% 2-propanol, 25% glycerol, 1.2M ammonium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
