Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4JGT

Structure and kinetic analysis of H2S production by human Mercaptopyruvate Sulfurtransferase

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000098	biological_process	sulfur amino acid catabolic process
A	0001822	biological_process	kidney development
A	0001889	biological_process	liver development
A	0004792	molecular_function	thiosulfate-cyanide sulfurtransferase activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005759	cellular_component	mitochondrial matrix
A	0009440	biological_process	cyanate catabolic process
A	0009636	biological_process	response to toxic substance
A	0016740	molecular_function	transferase activity
A	0016783	molecular_function	sulfurtransferase activity
A	0016784	molecular_function	3-mercaptopyruvate sulfurtransferase activity
A	0019346	biological_process	transsulfuration
A	0021510	biological_process	spinal cord development
A	0042802	molecular_function	identical protein binding
A	0043005	cellular_component	neuron projection
A	0045202	cellular_component	synapse
A	0070062	cellular_component	extracellular exosome
A	0070814	biological_process	hydrogen sulfide biosynthetic process
B	0000098	biological_process	sulfur amino acid catabolic process
B	0001822	biological_process	kidney development
B	0001889	biological_process	liver development
B	0004792	molecular_function	thiosulfate-cyanide sulfurtransferase activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005759	cellular_component	mitochondrial matrix
B	0009440	biological_process	cyanate catabolic process
B	0009636	biological_process	response to toxic substance
B	0016740	molecular_function	transferase activity
B	0016783	molecular_function	sulfurtransferase activity
B	0016784	molecular_function	3-mercaptopyruvate sulfurtransferase activity
B	0019346	biological_process	transsulfuration
B	0021510	biological_process	spinal cord development
B	0042802	molecular_function	identical protein binding
B	0043005	cellular_component	neuron projection
B	0045202	cellular_component	synapse
B	0070062	cellular_component	extracellular exosome
B	0070814	biological_process	hydrogen sulfide biosynthetic process
C	0000098	biological_process	sulfur amino acid catabolic process
C	0001822	biological_process	kidney development
C	0001889	biological_process	liver development
C	0004792	molecular_function	thiosulfate-cyanide sulfurtransferase activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005759	cellular_component	mitochondrial matrix
C	0009440	biological_process	cyanate catabolic process
C	0009636	biological_process	response to toxic substance
C	0016740	molecular_function	transferase activity
C	0016783	molecular_function	sulfurtransferase activity
C	0016784	molecular_function	3-mercaptopyruvate sulfurtransferase activity
C	0019346	biological_process	transsulfuration
C	0021510	biological_process	spinal cord development
C	0042802	molecular_function	identical protein binding
C	0043005	cellular_component	neuron projection
C	0045202	cellular_component	synapse
C	0070062	cellular_component	extracellular exosome
C	0070814	biological_process	hydrogen sulfide biosynthetic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PYR A 401

Chain	Residue
A	TRP36
A	HOH527
A	LEU38
A	TYR108
A	ARG188
A	ARG197
A	CSS248
A	GLY249
A	SER250
A	HOH521

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 A 402

Chain	Residue
A	SER66
A	ARG68
A	PRO77
A	GLY78
A	HIS81
A	HOH501

site_id	AC3
Number of Residues	9
Details	BINDING SITE FOR RESIDUE SO4 A 403

Chain	Residue
A	TRP15
A	ARG24
A	GLN27
A	HIS96
C	TRP15
C	ARG24
C	GLN27
C	HIS96
C	HOH614

site_id	AC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 A 404

Chain	Residue
A	GLN64
A	ARG68

site_id	AC5
Number of Residues	9
Details	BINDING SITE FOR RESIDUE PYR B 401

Chain	Residue
B	TRP36
B	TYR108
B	ARG188
B	ARG197
B	CSS248
B	GLY249
B	SER250
B	HOH538
B	HOH551

site_id	AC6
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 B 402

Chain	Residue
B	SER66
B	ASP67
B	ARG68
B	PRO77
B	GLY78
B	HIS81
B	HOH501

site_id	AC7
Number of Residues	9
Details	BINDING SITE FOR RESIDUE PYR C 401

Chain	Residue
C	TRP36
C	TYR108
C	ARG188
C	ARG197
C	CSS248
C	GLY249
C	SER250
C	HOH535
C	HOH547

site_id	AC8
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 C 402

Chain	Residue
C	SER66
C	ASP67
C	ARG68
C	PRO77
C	GLY78
C	HIS81
C	HOH501

site_id	AC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 C 403

Chain	Residue
C	ARG118
C	HIS123
C	TYR262
C	HOH606

site_id	BC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 C 404

Chain	Residue
C	ALA79
C	GLU80
C	HOH501
C	HOH506
C	HOH611

site_id	BC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 C 405

Chain	Residue
C	ALA25
C	GLY26
C	PRO28
C	HOH526

site_id	BC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL C 406

Chain	Residue
B	GLY207
C	HIS204
C	PRO206
C	GLY207

Functional Information from PROSITE/UniProt

site_id	PS00380
Number of Residues	12
Details	RHODANESE_1 Rhodanese signature 1. FeerHIPGAafF

Chain	Residue	Details
A	PHE49-PHE60

site_id	PS00683
Number of Residues	11
Details	RHODANESE_2 Rhodanese C-terminal signature. VpiYDGSWvEW

Chain	Residue	Details
A	VAL269-TRP279

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	119
Details	Domain: {"description":"Rhodanese 1","evidences":[{"source":"PROSITE-ProRule","id":"PRU00173","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	45
Details	Region: {"description":"Hinge"}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	3
Details	Active site: {"description":"Cysteine persulfide intermediate","evidences":[{"source":"PROSITE-ProRule","id":"PRU00173","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	3
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	6
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	2
Details	Modified residue: {"description":"N6-succinyllysine; alternate","evidences":[{"source":"UniProtKB","id":"Q99J99","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	6
Details	Modified residue: {"description":"N6-succinyllysine","evidences":[{"source":"UniProtKB","id":"Q99J99","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	3
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)