Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4JCL

Crystal structure of Alpha-CGT from Paenibacillus macerans at 1.7 Angstrom resolution

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004556	molecular_function	alpha-amylase activity
A	0005576	cellular_component	extracellular region
A	0005975	biological_process	carbohydrate metabolic process
A	0016740	molecular_function	transferase activity
A	0016757	molecular_function	glycosyltransferase activity
A	0030246	molecular_function	carbohydrate binding
A	0043169	molecular_function	cation binding
A	0043895	molecular_function	cyclomaltodextrin glucanotransferase activity
A	0046872	molecular_function	metal ion binding
A	2001070	molecular_function	starch binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA A 701

Chain	Residue
A	ASP27
A	ASP29
A	ASN32
A	ASN33
A	GLY51
A	ASP53
A	HOH839

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 702

Chain	Residue
A	GLU292
A	GLU299
A	HOH1085
A	HOH1086
A	ASN62
A	ASP264

site_id	AC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA A 703

Chain	Residue
A	ASN139
A	ILE190
A	ASP199
A	HIS233
A	HOH1052
A	HOH1053
A	HOH1054

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 704

Chain	Residue
A	ASP264
A	GLU289
A	GLU292
A	GLU299
A	HOH852
A	HOH857

site_id	AC5
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CL A 705

Chain	Residue
A	GLY427

site_id	AC6
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CL A 706

Chain	Residue
A	ALA495
A	ARG521

site_id	AC7
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CL A 707

Chain	Residue
A	ILE542

site_id	AC8
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 709

Chain	Residue
A	ILE190
A	TYR191
A	PHE236
A	GLY237
A	TYR627
A	TYR639
A	ASP640

site_id	AC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 710

Chain	Residue
A	LYS212
A	ASP216
A	SER245
A	HIS251
A	HOH817
A	HOH1257

site_id	BC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 711

Chain	Residue
A	TYR100
A	HIS140
A	ASP229
A	GOL716
A	EDO720
A	HOH1049

site_id	BC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 712

Chain	Residue
A	GLU81
A	GLN107
A	THR108
A	ASP110
A	GLY447
A	GOL713

site_id	BC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 713

Chain	Residue
A	GLU81
A	ASP104
A	GLN107
A	TYR158
A	GLY161
A	GOL712

site_id	BC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 714

Chain	Residue
A	ASP145
A	PHE151
A	ASP196
A	THR456
A	HOH1230
A	HOH1258

site_id	BC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL A 715

Chain	Residue
A	THR67
A	GLY70
A	VAL71
A	HIS128
A	HOH1044

site_id	BC6
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL A 716

Chain	Residue
A	LYS47
A	HIS98
A	TRP101
A	ASP372
A	ARG376
A	GOL711
A	EDO720
A	HOH1038
A	HOH1083

site_id	BC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 717

Chain	Residue
A	ASP29
A	ASP114
A	ASP117
A	HOH967
A	HOH1027
A	HOH1033

site_id	BC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO A 718

Chain	Residue
A	GLN19
A	SER77
A	HIS98
A	TYR100
A	ASP135
A	HOH1025

site_id	BC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO A 719

Chain	Residue
A	LYS106
A	SER213
A	TRP663
A	HOH1091
A	HOH1171

site_id	CC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO A 720

Chain	Residue
A	TRP101
A	ASP196
A	GOL711
A	GOL716
A	EDO727
A	TYR89

site_id	CC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE EDO A 721

Chain	Residue
A	GLU276
A	ASP316
A	LYS561

site_id	CC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO A 722

Chain	Residue
A	GLN334
A	ALA336
A	GLY337
A	SER338
A	HOH886
A	HOH1213

site_id	CC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE EDO A 723

Chain	Residue
A	EDO724
A	PEG729
A	HOH1260

site_id	CC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE EDO A 724

Chain	Residue
A	GLN629
A	TYR634
A	EDO723

site_id	CC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE EDO A 725

Chain	Residue
A	THR300
A	ASP303
A	EDO726
A	HOH861

site_id	CC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO A 726

Chain	Residue
A	GLN55
A	ILE58
A	ASN120
A	EDO725
A	HOH847
A	HOH950

site_id	CC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE EDO A 727

Chain	Residue
A	SER458
A	EDO720
A	HOH891

site_id	CC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE PEG A 728

Chain	Residue
A	GLU412
A	ARG413
A	TRP414
A	VAL415

site_id	DC1
Number of Residues	9
Details	BINDING SITE FOR RESIDUE PEG A 729

Chain	Residue
A	THR599
A	ASN600
A	TYR601
A	GLY602
A	THR603
A	ASN604
A	TYR634
A	EDO723
A	HOH1260

site_id	DC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PGE A 730

Chain	Residue
A	LYS302
A	VAL415
A	ASN416
A	ASN417
A	TYR443

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	80
Details	Domain: {"description":"IPT/TIG"}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	105
Details	Domain: {"description":"CBM20","evidences":[{"source":"PROSITE-ProRule","id":"PRU00594","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	137
Details	Region: {"description":"A1"}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	63
Details	Region: {"description":"B"}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	204
Details	Region: {"description":"A2"}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	88
Details	Region: {"description":"C"}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	86
Details	Region: {"description":"D"}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	103
Details	Region: {"description":"E"}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	1
Details	Active site: {"description":"Nucleophile","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	1
Details	Active site: {"description":"Proton donor","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	19
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI12
Number of Residues	1
Details	Site: {"description":"Transition state stabilizer","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)