4JCL
Crystal structure of Alpha-CGT from Paenibacillus macerans at 1.7 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-05 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.978 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.871, 78.559, 136.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.450 - 1.700 |
R-factor | 0.166 |
Rwork | 0.164 |
R-free | 0.20700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cgt |
RMSD bond length | 0.006 |
RMSD bond angle | 1.014 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.092 | |
Number of reflections | 72308 | |
<I/σ(I)> | 18.25 | |
Completeness [%] | 91.7 | 91.1 |
Redundancy | 8.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 30% PEG 8000, 0.1M IMIDAZOLE, 0.1M CALCIUM CLORIDE, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |