Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4JBW

Crystal structure of E. coli maltose transporter MalFGK2 in complex with its regulatory protein EIIAglc

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005886	cellular_component	plasma membrane
A	0008643	biological_process	carbohydrate transport
A	0015423	molecular_function	ABC-type maltose transporter activity
A	0015768	biological_process	maltose transport
A	0016020	cellular_component	membrane
A	0016887	molecular_function	ATP hydrolysis activity
A	0022857	molecular_function	transmembrane transporter activity
A	0032991	cellular_component	protein-containing complex
A	0034763	biological_process	negative regulation of transmembrane transport
A	0042956	biological_process	maltodextrin transmembrane transport
A	0043190	cellular_component	ATP-binding cassette (ABC) transporter complex
A	0055052	cellular_component	ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
A	0055085	biological_process	transmembrane transport
A	0140297	molecular_function	DNA-binding transcription factor binding
A	0140359	molecular_function	ABC-type transporter activity
A	1902344	biological_process	negative regulation of maltose transport
A	1904981	biological_process	maltose transmembrane transport
A	1990060	cellular_component	maltose transport complex
A	1990154	cellular_component	enzyme IIA-maltose transporter complex
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005886	cellular_component	plasma membrane
B	0008643	biological_process	carbohydrate transport
B	0015423	molecular_function	ABC-type maltose transporter activity
B	0015768	biological_process	maltose transport
B	0016020	cellular_component	membrane
B	0016887	molecular_function	ATP hydrolysis activity
B	0022857	molecular_function	transmembrane transporter activity
B	0032991	cellular_component	protein-containing complex
B	0034763	biological_process	negative regulation of transmembrane transport
B	0042956	biological_process	maltodextrin transmembrane transport
B	0043190	cellular_component	ATP-binding cassette (ABC) transporter complex
B	0055052	cellular_component	ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
B	0055085	biological_process	transmembrane transport
B	0140297	molecular_function	DNA-binding transcription factor binding
B	0140359	molecular_function	ABC-type transporter activity
B	1902344	biological_process	negative regulation of maltose transport
B	1904981	biological_process	maltose transmembrane transport
B	1990060	cellular_component	maltose transport complex
B	1990154	cellular_component	enzyme IIA-maltose transporter complex
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005886	cellular_component	plasma membrane
C	0008643	biological_process	carbohydrate transport
C	0015423	molecular_function	ABC-type maltose transporter activity
C	0015768	biological_process	maltose transport
C	0016020	cellular_component	membrane
C	0016887	molecular_function	ATP hydrolysis activity
C	0022857	molecular_function	transmembrane transporter activity
C	0032991	cellular_component	protein-containing complex
C	0034763	biological_process	negative regulation of transmembrane transport
C	0042956	biological_process	maltodextrin transmembrane transport
C	0043190	cellular_component	ATP-binding cassette (ABC) transporter complex
C	0055052	cellular_component	ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
C	0055085	biological_process	transmembrane transport
C	0140297	molecular_function	DNA-binding transcription factor binding
C	0140359	molecular_function	ABC-type transporter activity
C	1902344	biological_process	negative regulation of maltose transport
C	1904981	biological_process	maltose transmembrane transport
C	1990060	cellular_component	maltose transport complex
C	1990154	cellular_component	enzyme IIA-maltose transporter complex
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005886	cellular_component	plasma membrane
D	0008643	biological_process	carbohydrate transport
D	0015423	molecular_function	ABC-type maltose transporter activity
D	0015768	biological_process	maltose transport
D	0016020	cellular_component	membrane
D	0016887	molecular_function	ATP hydrolysis activity
D	0022857	molecular_function	transmembrane transporter activity
D	0032991	cellular_component	protein-containing complex
D	0034763	biological_process	negative regulation of transmembrane transport
D	0042956	biological_process	maltodextrin transmembrane transport
D	0043190	cellular_component	ATP-binding cassette (ABC) transporter complex
D	0055052	cellular_component	ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
D	0055085	biological_process	transmembrane transport
D	0140297	molecular_function	DNA-binding transcription factor binding
D	0140359	molecular_function	ABC-type transporter activity
D	1902344	biological_process	negative regulation of maltose transport
D	1904981	biological_process	maltose transmembrane transport
D	1990060	cellular_component	maltose transport complex
D	1990154	cellular_component	enzyme IIA-maltose transporter complex
F	0005515	molecular_function	protein binding
F	0005886	cellular_component	plasma membrane
F	0006974	biological_process	DNA damage response
F	0015423	molecular_function	ABC-type maltose transporter activity
F	0015768	biological_process	maltose transport
F	0016020	cellular_component	membrane
F	0034763	biological_process	negative regulation of transmembrane transport
F	0042956	biological_process	maltodextrin transmembrane transport
F	0043190	cellular_component	ATP-binding cassette (ABC) transporter complex
F	0055085	biological_process	transmembrane transport
F	1902344	biological_process	negative regulation of maltose transport
F	1904981	biological_process	maltose transmembrane transport
F	1990060	cellular_component	maltose transport complex
F	1990154	cellular_component	enzyme IIA-maltose transporter complex
G	0005515	molecular_function	protein binding
G	0005886	cellular_component	plasma membrane
G	0015423	molecular_function	ABC-type maltose transporter activity
G	0015768	biological_process	maltose transport
G	0016020	cellular_component	membrane
G	0034763	biological_process	negative regulation of transmembrane transport
G	0042956	biological_process	maltodextrin transmembrane transport
G	0043190	cellular_component	ATP-binding cassette (ABC) transporter complex
G	0055085	biological_process	transmembrane transport
G	1902344	biological_process	negative regulation of maltose transport
G	1904981	biological_process	maltose transmembrane transport
G	1990060	cellular_component	maltose transport complex
G	1990154	cellular_component	enzyme IIA-maltose transporter complex
H	0005515	molecular_function	protein binding
H	0005886	cellular_component	plasma membrane
H	0006974	biological_process	DNA damage response
H	0015423	molecular_function	ABC-type maltose transporter activity
H	0015768	biological_process	maltose transport
H	0016020	cellular_component	membrane
H	0034763	biological_process	negative regulation of transmembrane transport
H	0042956	biological_process	maltodextrin transmembrane transport
H	0043190	cellular_component	ATP-binding cassette (ABC) transporter complex
H	0055085	biological_process	transmembrane transport
H	1902344	biological_process	negative regulation of maltose transport
H	1904981	biological_process	maltose transmembrane transport
H	1990060	cellular_component	maltose transport complex
H	1990154	cellular_component	enzyme IIA-maltose transporter complex
I	0005515	molecular_function	protein binding
I	0005886	cellular_component	plasma membrane
I	0015423	molecular_function	ABC-type maltose transporter activity
I	0015768	biological_process	maltose transport
I	0016020	cellular_component	membrane
I	0034763	biological_process	negative regulation of transmembrane transport
I	0042956	biological_process	maltodextrin transmembrane transport
I	0043190	cellular_component	ATP-binding cassette (ABC) transporter complex
I	0055085	biological_process	transmembrane transport
I	1902344	biological_process	negative regulation of maltose transport
I	1904981	biological_process	maltose transmembrane transport
I	1990060	cellular_component	maltose transport complex
I	1990154	cellular_component	enzyme IIA-maltose transporter complex
M	0005515	molecular_function	protein binding
M	0005737	cellular_component	cytoplasm
M	0005829	cellular_component	cytosol
M	0009401	biological_process	phosphoenolpyruvate-dependent sugar phosphotransferase system
M	0009898	cellular_component	cytoplasmic side of plasma membrane
M	0016020	cellular_component	membrane
M	0016301	molecular_function	kinase activity
M	0034763	biological_process	negative regulation of transmembrane transport
M	0043610	biological_process	regulation of carbohydrate utilization
M	0045912	biological_process	negative regulation of carbohydrate metabolic process
M	0046872	molecular_function	metal ion binding
M	1902344	biological_process	negative regulation of maltose transport
M	1902495	cellular_component	transmembrane transporter complex
M	1990154	cellular_component	enzyme IIA-maltose transporter complex
N	0005515	molecular_function	protein binding
N	0005737	cellular_component	cytoplasm
N	0005829	cellular_component	cytosol
N	0009401	biological_process	phosphoenolpyruvate-dependent sugar phosphotransferase system
N	0009898	cellular_component	cytoplasmic side of plasma membrane
N	0016020	cellular_component	membrane
N	0016301	molecular_function	kinase activity
N	0034763	biological_process	negative regulation of transmembrane transport
N	0043610	biological_process	regulation of carbohydrate utilization
N	0045912	biological_process	negative regulation of carbohydrate metabolic process
N	0046872	molecular_function	metal ion binding
N	1902344	biological_process	negative regulation of maltose transport
N	1902495	cellular_component	transmembrane transporter complex
N	1990154	cellular_component	enzyme IIA-maltose transporter complex
O	0005515	molecular_function	protein binding
O	0005737	cellular_component	cytoplasm
O	0005829	cellular_component	cytosol
O	0009401	biological_process	phosphoenolpyruvate-dependent sugar phosphotransferase system
O	0009898	cellular_component	cytoplasmic side of plasma membrane
O	0016020	cellular_component	membrane
O	0016301	molecular_function	kinase activity
O	0034763	biological_process	negative regulation of transmembrane transport
O	0043610	biological_process	regulation of carbohydrate utilization
O	0045912	biological_process	negative regulation of carbohydrate metabolic process
O	0046872	molecular_function	metal ion binding
O	1902344	biological_process	negative regulation of maltose transport
O	1902495	cellular_component	transmembrane transporter complex
O	1990154	cellular_component	enzyme IIA-maltose transporter complex
P	0005515	molecular_function	protein binding
P	0005737	cellular_component	cytoplasm
P	0005829	cellular_component	cytosol
P	0009401	biological_process	phosphoenolpyruvate-dependent sugar phosphotransferase system
P	0009898	cellular_component	cytoplasmic side of plasma membrane
P	0016020	cellular_component	membrane
P	0016301	molecular_function	kinase activity
P	0034763	biological_process	negative regulation of transmembrane transport
P	0043610	biological_process	regulation of carbohydrate utilization
P	0045912	biological_process	negative regulation of carbohydrate metabolic process
P	0046872	molecular_function	metal ion binding
P	1902344	biological_process	negative regulation of maltose transport
P	1902495	cellular_component	transmembrane transporter complex
P	1990154	cellular_component	enzyme IIA-maltose transporter complex

Functional Information from PDB Data

site_id	AC1
Number of Residues	8
Details	BINDING SITE FOR RESIDUE PGV F 4001

Chain	Residue
F	CYS304
F	LEU305
F	TRP308
F	TYR400
F	PRO410
F	PHE414
G	ARG12
G	THR16

site_id	AC2
Number of Residues	9
Details	BINDING SITE FOR RESIDUE PGV H 601

Chain	Residue
H	CYS304
H	LEU305
H	TRP308
H	TYR400
H	PRO410
H	ASN413
I	ARG12
I	THR16
H	VAL301

Functional Information from PROSITE/UniProt

site_id	PS00211
Number of Residues	15
Details	ABC_TRANSPORTER_1 ABC transporters family signature. LSGGQRQRVAIGRTL

Chain	Residue	Details
A	LEU134-LEU148

site_id	PS00371
Number of Residues	13
Details	PTS_EIIA_TYPE_1_HIS PTS EIIA domains phosphorylation site signature 1. GveLFVHFGIdTV

Chain	Residue	Details
M	GLY84-VAL96

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Tele-phosphohistidine intermediate; for EIIA activity => ECO:0000255\|PROSITE-ProRule:PRU00416, ECO:0000269\|PubMed:2657735, ECO:0000269\|PubMed:6383826, ECO:0000305\|PubMed:1961703, ECO:0000305\|PubMed:9405042

Chain	Residue	Details
M	HIS90
H	LEU506-ASP514
N	HIS90
O	HIS90
P	HIS90
I	MET1-LEU18
I	SER103-LYS123
I	LEU172-ARG204
I	ALA281-GLY296
H	LYS393-PRO425

site_id	SWS_FT_FI2
Number of Residues	8
Details	BINDING: BINDING => ECO:0000269\|PubMed:2657735, ECO:0000269\|PubMed:8170944, ECO:0000269\|PubMed:9405042, ECO:0000305\|PubMed:1961703

Chain	Residue	Details
M	HIS75
I	ILE151-ALA171
I	LEU205-ALA225
I	TRP260-LEU280
H	VAL319-PHE336
H	THR370-LEU392
H	LEU426-PRO452
H	PHE484-ASN505
M	HIS90
N	HIS75
N	HIS90
O	HIS75
O	HIS90
P	HIS75
P	HIS90
I	GLY124-PHE144

site_id	SWS_FT_FI3
Number of Residues	4
Details	SITE: Important for phospho-donor activity => ECO:0000269\|PubMed:2657735

Chain	Residue	Details
M	HIS75
N	HIS75
O	HIS75
P	HIS75
I	ASP145-TYR150
I	ALA226-LEU259
H	LYS337-ARG369
H	ASP453-ASP483

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: Phosphohistidine; by HPr => ECO:0000269\|PubMed:2657735

Chain	Residue	Details
M	HIS90
N	HIS90
O	HIS90
P	HIS90

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)