4JBW
Crystal structure of E. coli maltose transporter MalFGK2 in complex with its regulatory protein EIIAglc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-12 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03320 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 94.529, 208.448, 347.477 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.975 - 3.913 |
R-factor | 0.2288 |
Rwork | 0.226 |
R-free | 0.28120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f3g 3fh6 and 3PV0 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.646 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 4.060 |
High resolution limit [Å] | 3.913 | 3.913 |
Number of reflections | 59277 | |
<I/σ(I)> | 13.8 | |
Completeness [%] | 95.0 | 96.5 |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 295 | 13% PEG monomethylether 2000, 100mM Sodium cacodylate pH 5.6, 100mM Non Detergent Sulfobetaine (NDSB-256), VAPOR DIFFUSION, SITTING DROP, temperature 295K |