4HQJ

Crystal structure of Na+,K+-ATPase in the Na+-bound state

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0002028	biological_process	regulation of sodium ion transport
A	0005215	molecular_function	transporter activity
A	0005391	molecular_function	P-type sodium:potassium-exchanging transporter activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005765	cellular_component	lysosomal membrane
A	0005886	cellular_component	plasma membrane
A	0005890	cellular_component	sodium:potassium-exchanging ATPase complex
A	0006813	biological_process	potassium ion transport
A	0006883	biological_process	intracellular sodium ion homeostasis
A	0008556	molecular_function	P-type potassium transmembrane transporter activity
A	0010248	biological_process	establishment or maintenance of transmembrane electrochemical gradient
A	0016020	cellular_component	membrane
A	0016323	cellular_component	basolateral plasma membrane
A	0016887	molecular_function	ATP hydrolysis activity
A	0030007	biological_process	intracellular potassium ion homeostasis
A	0030424	cellular_component	axon
A	0030955	molecular_function	potassium ion binding
A	0031402	molecular_function	sodium ion binding
A	0036376	biological_process	sodium ion export across plasma membrane
A	0042383	cellular_component	sarcolemma
A	0042470	cellular_component	melanosome
A	0051117	molecular_function	ATPase binding
A	0055085	biological_process	transmembrane transport
A	0071805	biological_process	potassium ion transmembrane transport
A	0086009	biological_process	membrane repolarization
A	1902600	biological_process	proton transmembrane transport
A	1990573	biological_process	potassium ion import across plasma membrane
B	0001671	molecular_function	ATPase activator activity
B	0005391	molecular_function	P-type sodium:potassium-exchanging transporter activity
B	0005515	molecular_function	protein binding
B	0005765	cellular_component	lysosomal membrane
B	0005886	cellular_component	plasma membrane
B	0005890	cellular_component	sodium:potassium-exchanging ATPase complex
B	0006813	biological_process	potassium ion transport
B	0006814	biological_process	sodium ion transport
B	0006874	biological_process	intracellular calcium ion homeostasis
B	0006883	biological_process	intracellular sodium ion homeostasis
B	0010248	biological_process	establishment or maintenance of transmembrane electrochemical gradient
B	0010468	biological_process	regulation of gene expression
B	0010882	biological_process	regulation of cardiac muscle contraction by calcium ion signaling
B	0014704	cellular_component	intercalated disc
B	0016020	cellular_component	membrane
B	0016323	cellular_component	basolateral plasma membrane
B	0016324	cellular_component	apical plasma membrane
B	0016328	cellular_component	lateral plasma membrane
B	0019901	molecular_function	protein kinase binding
B	0030007	biological_process	intracellular potassium ion homeostasis
B	0030315	cellular_component	T-tubule
B	0030674	molecular_function	protein-macromolecule adaptor activity
B	0035725	biological_process	sodium ion transmembrane transport
B	0036126	cellular_component	sperm flagellum
B	0036376	biological_process	sodium ion export across plasma membrane
B	0042383	cellular_component	sarcolemma
B	0046034	biological_process	ATP metabolic process
B	0050821	biological_process	protein stabilization
B	0051117	molecular_function	ATPase binding
B	0055085	biological_process	transmembrane transport
B	0055119	biological_process	relaxation of cardiac muscle
B	0060048	biological_process	cardiac muscle contraction
B	0072659	biological_process	protein localization to plasma membrane
B	0086009	biological_process	membrane repolarization
B	0141109	molecular_function	transporter activator activity
B	1901381	biological_process	positive regulation of potassium ion transmembrane transport
B	1902307	biological_process	positive regulation of sodium ion transmembrane transport
B	1902600	biological_process	proton transmembrane transport
B	1903169	biological_process	regulation of calcium ion transmembrane transport
B	1903278	biological_process	positive regulation of sodium ion export across plasma membrane
B	1903288	biological_process	positive regulation of potassium ion import across plasma membrane
B	1990573	biological_process	potassium ion import across plasma membrane
C	0000166	molecular_function	nucleotide binding
C	0002028	biological_process	regulation of sodium ion transport
C	0005215	molecular_function	transporter activity
C	0005391	molecular_function	P-type sodium:potassium-exchanging transporter activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005765	cellular_component	lysosomal membrane
C	0005886	cellular_component	plasma membrane
C	0005890	cellular_component	sodium:potassium-exchanging ATPase complex
C	0006813	biological_process	potassium ion transport
C	0006883	biological_process	intracellular sodium ion homeostasis
C	0008556	molecular_function	P-type potassium transmembrane transporter activity
C	0010248	biological_process	establishment or maintenance of transmembrane electrochemical gradient
C	0016020	cellular_component	membrane
C	0016323	cellular_component	basolateral plasma membrane
C	0016887	molecular_function	ATP hydrolysis activity
C	0030007	biological_process	intracellular potassium ion homeostasis
C	0030424	cellular_component	axon
C	0030955	molecular_function	potassium ion binding
C	0031402	molecular_function	sodium ion binding
C	0036376	biological_process	sodium ion export across plasma membrane
C	0042383	cellular_component	sarcolemma
C	0042470	cellular_component	melanosome
C	0051117	molecular_function	ATPase binding
C	0055085	biological_process	transmembrane transport
C	0071805	biological_process	potassium ion transmembrane transport
C	0086009	biological_process	membrane repolarization
C	1902600	biological_process	proton transmembrane transport
C	1990573	biological_process	potassium ion import across plasma membrane
D	0001671	molecular_function	ATPase activator activity
D	0005391	molecular_function	P-type sodium:potassium-exchanging transporter activity
D	0005515	molecular_function	protein binding
D	0005765	cellular_component	lysosomal membrane
D	0005886	cellular_component	plasma membrane
D	0005890	cellular_component	sodium:potassium-exchanging ATPase complex
D	0006813	biological_process	potassium ion transport
D	0006814	biological_process	sodium ion transport
D	0006874	biological_process	intracellular calcium ion homeostasis
D	0006883	biological_process	intracellular sodium ion homeostasis
D	0010248	biological_process	establishment or maintenance of transmembrane electrochemical gradient
D	0010468	biological_process	regulation of gene expression
D	0010882	biological_process	regulation of cardiac muscle contraction by calcium ion signaling
D	0014704	cellular_component	intercalated disc
D	0016020	cellular_component	membrane
D	0016323	cellular_component	basolateral plasma membrane
D	0016324	cellular_component	apical plasma membrane
D	0016328	cellular_component	lateral plasma membrane
D	0019901	molecular_function	protein kinase binding
D	0030007	biological_process	intracellular potassium ion homeostasis
D	0030315	cellular_component	T-tubule
D	0030674	molecular_function	protein-macromolecule adaptor activity
D	0035725	biological_process	sodium ion transmembrane transport
D	0036126	cellular_component	sperm flagellum
D	0036376	biological_process	sodium ion export across plasma membrane
D	0042383	cellular_component	sarcolemma
D	0046034	biological_process	ATP metabolic process
D	0050821	biological_process	protein stabilization
D	0051117	molecular_function	ATPase binding
D	0055085	biological_process	transmembrane transport
D	0055119	biological_process	relaxation of cardiac muscle
D	0060048	biological_process	cardiac muscle contraction
D	0072659	biological_process	protein localization to plasma membrane
D	0086009	biological_process	membrane repolarization
D	0141109	molecular_function	transporter activator activity
D	1901381	biological_process	positive regulation of potassium ion transmembrane transport
D	1902307	biological_process	positive regulation of sodium ion transmembrane transport
D	1902600	biological_process	proton transmembrane transport
D	1903169	biological_process	regulation of calcium ion transmembrane transport
D	1903278	biological_process	positive regulation of sodium ion export across plasma membrane
D	1903288	biological_process	positive regulation of potassium ion import across plasma membrane
D	1990573	biological_process	potassium ion import across plasma membrane
E	0001671	molecular_function	ATPase activator activity
E	0005515	molecular_function	protein binding
E	0005890	cellular_component	sodium:potassium-exchanging ATPase complex
E	0006811	biological_process	monoatomic ion transport
E	0006883	biological_process	intracellular sodium ion homeostasis
E	0010248	biological_process	establishment or maintenance of transmembrane electrochemical gradient
E	0016020	cellular_component	membrane
E	0030007	biological_process	intracellular potassium ion homeostasis
E	0030674	molecular_function	protein-macromolecule adaptor activity
E	0036376	biological_process	sodium ion export across plasma membrane
E	0043269	biological_process	regulation of monoatomic ion transport
E	0055085	biological_process	transmembrane transport
E	0099106	molecular_function	ion channel regulator activity
E	1902600	biological_process	proton transmembrane transport
E	1990573	biological_process	potassium ion import across plasma membrane
G	0001671	molecular_function	ATPase activator activity
G	0005515	molecular_function	protein binding
G	0005890	cellular_component	sodium:potassium-exchanging ATPase complex
G	0006811	biological_process	monoatomic ion transport
G	0006883	biological_process	intracellular sodium ion homeostasis
G	0010248	biological_process	establishment or maintenance of transmembrane electrochemical gradient
G	0016020	cellular_component	membrane
G	0030007	biological_process	intracellular potassium ion homeostasis
G	0030674	molecular_function	protein-macromolecule adaptor activity
G	0036376	biological_process	sodium ion export across plasma membrane
G	0043269	biological_process	regulation of monoatomic ion transport
G	0055085	biological_process	transmembrane transport
G	0099106	molecular_function	ion channel regulator activity
G	1902600	biological_process	proton transmembrane transport
G	1990573	biological_process	potassium ion import across plasma membrane

Functional Information from PDB Data

site_id	AC1
Number of Residues	16
Details	BINDING SITE FOR RESIDUE ADP A 1101

Chain	Residue
A	ASP443
A	LEU546
A	GLY611
A	ASP612
A	HIS613
A	ARG685
A	ASN713
A	ALF1102
A	SER445
A	GLU446
A	PHE475
A	SER477
A	GLN482
A	LYS501
A	GLY502
A	ARG544

---

site_id	AC2
Number of Residues	9
Details	BINDING SITE FOR RESIDUE ALF A 1102

Chain	Residue
A	ASP369
A	LYS370
A	THR371
A	THR610
A	GLY611
A	ASP612
A	LYS691
A	ADP1101
A	MG1103

---

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG A 1103

Chain	Residue
A	ASP369
A	THR371
A	ASP710
A	ALF1102

---

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE NA A 1104

Chain	Residue
A	VAL322
A	ALA323
A	VAL325
A	GLU327
A	ASP804

---

site_id	AC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE NA A 1105

Chain	Residue
A	ALA323
A	SER775
A	ASN776
A	GLU779
A	ASP804
A	ASP808

---

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE NA A 1106

Chain	Residue
A	TYR771
A	THR774
A	SER775
A	GLN854
A	GLN923
A	ASP926

---

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CLR A 1107

Chain	Residue
A	TRP924
A	LEU951
A	TRP981
A	PHE982
A	PHE985
G	PHE33

---

site_id	AC8
Number of Residues	10
Details	BINDING SITE FOR RESIDUE CLR A 1108

Chain	Residue
A	PHE860
A	VAL864
A	GLU868
A	THR979
A	CYS983
A	PRO986
B	THR53
B	VAL56
B	MET57
B	LEU59

---

site_id	AC9
Number of Residues	20
Details	BINDING SITE FOR RESIDUE ADP C 1101

Chain	Residue
C	THR371
C	ASP443
C	SER445
C	GLU446
C	PHE475
C	SER477
C	GLN482
C	LYS501
C	GLY502
C	ALA503
C	ARG544
C	LEU546
C	THR610
C	GLY611
C	ASP612
C	HIS613
C	ARG685
C	ASN713
C	ALF1102
C	MG1103

---

site_id	BC1
Number of Residues	8
Details	BINDING SITE FOR RESIDUE ALF C 1102

Chain	Residue
C	ASP369
C	LYS370
C	THR371
C	THR610
C	GLY611
C	ASP612
C	ADP1101
C	MG1103

---

site_id	BC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG C 1103

Chain	Residue
C	ASP369
C	THR371
C	ASP710
C	ADP1101
C	ALF1102

---

site_id	BC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE NA C 1104

Chain	Residue
C	ASP804
C	VAL322
C	ALA323
C	VAL325
C	GLU327

---

site_id	BC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE NA C 1105

Chain	Residue
C	ALA323
C	SER775
C	ASN776
C	GLU779
C	ASP804
C	ASP808

---

site_id	BC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE NA C 1106

Chain	Residue
C	TYR771
C	THR774
C	SER775
C	GLN854
C	GLN923
C	ASP926

---

site_id	BC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CLR C 1107

Chain	Residue
C	TRP924
C	LEU951
C	TYR963
C	TRP981
E	PHE33

---

site_id	BC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CLR C 1108

Chain	Residue
C	PHE860
C	GLU868
C	THR979
C	LEU989

---

site_id	BC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CLR D 400

Chain	Residue
C	SER844
D	TYR39
D	TYR43

---

Functional Information from PROSITE/UniProt

site_id	PS00154
Number of Residues	7
Details	ATPASE_E1_E2 E1-E2 ATPases phosphorylation site. DKTGTLT

Chain	Residue	Details
A	ASP369-THR375

---

site_id	PS00390
Number of Residues	21
Details	ATPASE_NA_K_BETA_1 Sodium and potassium ATPases beta subunits signature 1. WkkFiWNsekkefLGRTggsW

Chain	Residue	Details
B	TRP12-TRP32

---

site_id	PS00391
Number of Residues	16
Details	ATPASE_NA_K_BETA_2 Sodium and potassium ATPases beta subunits signature 2. RkvCRsrlewLgnCSG

Chain	Residue	Details
B	ARG146-GLY161

---

site_id	PS01310
Number of Residues	14
Details	FXYD FXYD family signature. DpFyYDyeTVRngG

Chain	Residue	Details
G	ASP17-GLY30

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	424
Details	Transmembrane: {"description":"Helical","evidences":[{"source":"PubMed","id":"35380894","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7WYT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	162
Details	Topological domain: {"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	1230
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	Region: {"description":"Phosphoinositide-3 kinase binding","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	38
Details	Region: {"description":"Disordered","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	242
Details	Region: {"description":"Mediates interaction with SCN7A","evidences":[{"source":"UniProtKB","id":"Q8VDN2","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	Active site: {"description":"4-aspartylphosphate intermediate","evidences":[{"source":"UniProtKB","id":"P19156","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	28
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P05023","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"18075585","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"35380894","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3B8E","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3KDP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7WYT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	14
Details	Binding site: {"evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"journal article","publicationDate":"2013","firstPage":"201","lastPage":"206","volume":"502","journal":"Nature","title":"Crystal structure of a Na1-bound Na1,K1-ATPase preceding the E1P state.","authors":["Kanai R.","Ogawa H.","Vilsen B.","Cornelius F.","Toyoshima C."]}},{"source":"PDB","id":"3WGU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3WGV","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"journal article","publicationDate":"2013","firstPage":"201","lastPage":"206","volume":"502","journal":"Nature","title":"Crystal structure of a Na1-bound Na1,K1-ATPase preceding the E1P state.","authors":["Kanai R.","Ogawa H.","Vilsen B.","Cornelius F.","Toyoshima C."]}},{"source":"PDB","id":"3WGV","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"journal article","publicationDate":"2013","firstPage":"201","lastPage":"206","volume":"502","journal":"Nature","title":"Crystal structure of a Na1-bound Na1,K1-ATPase preceding the E1P state.","authors":["Kanai R.","Ogawa H.","Vilsen B.","Cornelius F.","Toyoshima C."]}},{"source":"PDB","id":"3WGU","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"35380894","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7WYT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	2
Details	Binding site: {"description":"covalent","evidences":[{"source":"UniProtKB","id":"P05023","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	8
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P06685","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	6
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"Q8VDN2","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	2
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"UniProtKB","id":"Q8VDN2","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI18
Number of Residues	2
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"UniProtKB","id":"P05023","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI19
Number of Residues	2
Details	Modified residue: {"description":"N6-succinyllysine","evidences":[{"source":"UniProtKB","id":"Q8VDN2","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI20
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine; by PKA","evidences":[{"source":"UniProtKB","id":"P06685","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI21
Number of Residues	54
Details	Transmembrane: {"description":"Helical; Signal-anchor for type II membrane protein","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI22
Number of Residues	480
Details	Topological domain: {"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI23
Number of Residues	224
Details	Region: {"description":"immunoglobulin-like","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI24
Number of Residues	2
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"UniProtKB","id":"P14094","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI25
Number of Residues	6
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---