Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4HB4

Crystal structure of CRM1 inhibitor Leptomycin B in complex with CRM1(537DLTVK541/GLCEQ)-Ran-RanBP1

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000054	biological_process	ribosomal subunit export from nucleus
A	0000055	biological_process	ribosomal large subunit export from nucleus
A	0000056	biological_process	ribosomal small subunit export from nucleus
A	0000070	biological_process	mitotic sister chromatid segregation
A	0000278	biological_process	mitotic cell cycle
A	0000287	molecular_function	magnesium ion binding
A	0000785	cellular_component	chromatin
A	0003682	molecular_function	chromatin binding
A	0003723	molecular_function	RNA binding
A	0003924	molecular_function	GTPase activity
A	0003925	molecular_function	G protein activity
A	0005049	molecular_function	nuclear export signal receptor activity
A	0005515	molecular_function	protein binding
A	0005525	molecular_function	GTP binding
A	0005634	cellular_component	nucleus
A	0005635	cellular_component	nuclear envelope
A	0005643	cellular_component	nuclear pore
A	0005654	cellular_component	nucleoplasm
A	0005730	cellular_component	nucleolus
A	0005737	cellular_component	cytoplasm
A	0005814	cellular_component	centriole
A	0005829	cellular_component	cytosol
A	0006259	biological_process	DNA metabolic process
A	0006606	biological_process	protein import into nucleus
A	0006611	biological_process	protein export from nucleus
A	0006913	biological_process	nucleocytoplasmic transport
A	0007052	biological_process	mitotic spindle organization
A	0015031	biological_process	protein transport
A	0016020	cellular_component	membrane
A	0016032	biological_process	viral process
A	0016787	molecular_function	hydrolase activity
A	0019003	molecular_function	GDP binding
A	0030496	cellular_component	midbody
A	0032092	biological_process	positive regulation of protein binding
A	0032991	cellular_component	protein-containing complex
A	0035281	biological_process	pre-miRNA export from nucleus
A	0042307	biological_process	positive regulation of protein import into nucleus
A	0042470	cellular_component	melanosome
A	0042565	cellular_component	RNA nuclear export complex
A	0045296	molecular_function	cadherin binding
A	0046039	biological_process	GTP metabolic process
A	0046872	molecular_function	metal ion binding
A	0046982	molecular_function	protein heterodimerization activity
A	0051301	biological_process	cell division
A	0055037	cellular_component	recycling endosome
A	0061015	biological_process	snRNA import into nucleus
A	0070062	cellular_component	extracellular exosome
A	0070883	molecular_function	pre-miRNA binding
A	0090543	cellular_component	Flemming body
A	1902570	biological_process	protein localization to nucleolus
B	0006913	biological_process	nucleocytoplasmic transport
B	0046907	biological_process	intracellular transport
C	0000055	biological_process	ribosomal large subunit export from nucleus
C	0000056	biological_process	ribosomal small subunit export from nucleus
C	0000776	cellular_component	kinetochore
C	0005049	molecular_function	nuclear export signal receptor activity
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005816	cellular_component	spindle pole body
C	0006406	biological_process	mRNA export from nucleus
C	0006409	biological_process	tRNA export from nucleus
C	0006611	biological_process	protein export from nucleus
C	0006886	biological_process	intracellular protein transport
C	0015031	biological_process	protein transport
C	0017070	molecular_function	U6 snRNA binding
C	0030619	molecular_function	U1 snRNA binding
C	0030620	molecular_function	U2 snRNA binding
C	0030621	molecular_function	U4 snRNA binding
C	0030623	molecular_function	U5 snRNA binding
C	0031267	molecular_function	small GTPase binding
C	0034501	biological_process	protein localization to kinetochore
C	0048471	cellular_component	perinuclear region of cytoplasm
C	0051168	biological_process	nuclear export
C	0051170	biological_process	import into nucleus
C	0061608	molecular_function	nuclear import signal receptor activity
C	0071528	biological_process	tRNA re-export from nucleus

Functional Information from PDB Data

site_id	AC1
Number of Residues	31
Details	BINDING SITE FOR RESIDUE GNP A 301

Chain	Residue
A	ASP18
A	LYS37
A	LYS38
A	TYR39
A	ALA41
A	THR42
A	GLY68
A	GLN69
A	ASN122
A	LYS123
A	ASP125
A	GLY19
A	ILE126
A	SER150
A	ALA151
A	LYS152
A	MG302
A	HOH420
A	HOH422
A	HOH473
A	HOH484
A	HOH525
A	GLY20
A	HOH541
A	HOH545
A	THR21
A	GLY22
A	LYS23
A	THR24
A	THR25
A	GLU36

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG A 302

Chain	Residue
A	THR24
A	THR42
A	GNP301
A	HOH541
A	HOH545

site_id	AC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE EDO A 303

Chain	Residue
A	ASN103
A	ASP107
A	HOH464

site_id	AC4
Number of Residues	10
Details	BINDING SITE FOR RESIDUE LMB C 1101

Chain	Residue
C	LYS525
C	CYS539
C	GLU540
C	LYS548
C	ALA552
C	HIS569
C	PHE572
C	THR575
C	LYS579
C	PHE583

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL C 1102

Chain	Residue
C	LEU842
C	ASN846
C	TRP891
C	HOH1764
C	HOH1819

site_id	AC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE EDO C 1103

Chain	Residue
C	TYR967
C	GLN988
C	SER992

site_id	AC7
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CL C 1104

Chain	Residue
C	GLU1009

site_id	AC8
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CL C 1105

Chain	Residue
C	LYS605
C	TYR608

site_id	AC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CL C 1106

Chain	Residue
C	PRO61
C	GLN62
C	HOH1361

site_id	BC1
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CL C 1107

Chain	Residue
C	ARG227

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269\|PubMed:10078529, ECO:0000269\|PubMed:10353245, ECO:0000269\|PubMed:10367892, ECO:0000269\|PubMed:11832950, ECO:0000269\|PubMed:18611384, ECO:0000269\|PubMed:19389996, ECO:0000269\|PubMed:19505478, ECO:0000269\|PubMed:24449914, ECO:0000269\|PubMed:24915079, ECO:0000269\|PubMed:26272610, ECO:0000269\|PubMed:26349033, ECO:0000269\|PubMed:28282025, ECO:0000269\|PubMed:7885480, ECO:0007744\|PDB:1IBR, ECO:0007744\|PDB:1K5G, ECO:0007744\|PDB:1RRP, ECO:0007744\|PDB:3CH5, ECO:0007744\|PDB:3GJ0, ECO:0007744\|PDB:3GJ3, ECO:0007744\|PDB:3GJ4, ECO:0007744\|PDB:3GJ5, ECO:0007744\|PDB:3GJ6, ECO:0007744\|PDB:3GJ7, ECO:0007744\|PDB:3GJ8, ECO:0007744\|PDB:3GJX, ECO:0007744\|PDB:3NBY, ECO:0007744\|PDB:3NBZ, ECO:0007744\|PDB:3NC0, ECO:0007744\|PDB:3NC1, ECO:0007744\|PDB:3ZJY, ECO:0007744\|PDB:4C0Q, ECO:0007744\|PDB:4OL0, ECO:0007744\|PDB:5CIQ, ECO:0007744\|PDB:5CIT, ECO:0007744\|PDB:5CIW, ECO:0007744\|PDB:5CLQ, ECO:0007744\|PDB:5DH9, ECO:0007744\|PDB:5DHA, ECO:0007744\|PDB:5DHF, ECO:0007744\|PDB:5DI9, ECO:0007744\|PDB:5DIF, ECO:0007744\|PDB:5DIS, ECO:0007744\|PDB:5DLQ, ECO:0007744\|PDB:5UWH, ECO:0007744\|PDB:5UWI, ECO:0007744\|PDB:5UWJ, ECO:0007744\|PDB:5UWO, ECO:0007744\|PDB:5UWP, ECO:0007744\|PDB:5UWQ, ECO:0007744\|PDB:5UWR, ECO:0007744\|PDB:5UWS, ECO:0007744\|PDB:5UWT, ECO:0007744\|PDB:5UWU, ECO:0007744\|PDB:5UWW

Chain	Residue	Details
A	ASP18

site_id	SWS_FT_FI2
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269\|PubMed:10078529, ECO:0000269\|PubMed:10353245, ECO:0000269\|PubMed:10367892, ECO:0000269\|PubMed:11832950, ECO:0000269\|PubMed:19389996, ECO:0000269\|PubMed:24449914, ECO:0000269\|PubMed:24915079, ECO:0000269\|PubMed:26272610, ECO:0000269\|PubMed:26349033, ECO:0000269\|PubMed:28282025, ECO:0000269\|PubMed:7885480, ECO:0007744\|PDB:1IBR, ECO:0007744\|PDB:1K5D, ECO:0007744\|PDB:1QBK, ECO:0007744\|PDB:1RRP, ECO:0007744\|PDB:3GJX, ECO:0007744\|PDB:3NBY, ECO:0007744\|PDB:3NBZ, ECO:0007744\|PDB:3NC0, ECO:0007744\|PDB:3NC1, ECO:0007744\|PDB:3ZJY, ECO:0007744\|PDB:4C0Q, ECO:0007744\|PDB:4OL0, ECO:0007744\|PDB:5CLL, ECO:0007744\|PDB:5CLQ, ECO:0007744\|PDB:5DH9, ECO:0007744\|PDB:5DHA, ECO:0007744\|PDB:5DHF, ECO:0007744\|PDB:5DI9, ECO:0007744\|PDB:5DIF, ECO:0007744\|PDB:5DIS, ECO:0007744\|PDB:5DLQ, ECO:0007744\|PDB:5JLJ, ECO:0007744\|PDB:5UWH, ECO:0007744\|PDB:5UWI, ECO:0007744\|PDB:5UWJ, ECO:0007744\|PDB:5UWO, ECO:0007744\|PDB:5UWP, ECO:0007744\|PDB:5UWQ, ECO:0007744\|PDB:5UWR, ECO:0007744\|PDB:5UWS, ECO:0007744\|PDB:5UWT, ECO:0007744\|PDB:5UWU, ECO:0007744\|PDB:5UWW

Chain	Residue	Details
A	GLU36

site_id	SWS_FT_FI3
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269\|PubMed:10078529, ECO:0000269\|PubMed:10353245, ECO:0000269\|PubMed:10367892, ECO:0000269\|PubMed:11832950, ECO:0000269\|PubMed:18611384, ECO:0000269\|PubMed:19389996, ECO:0000269\|PubMed:19505478, ECO:0000269\|PubMed:24449914, ECO:0000269\|PubMed:24915079, ECO:0000269\|PubMed:26272610, ECO:0000269\|PubMed:26349033, ECO:0000269\|PubMed:28282025, ECO:0000269\|PubMed:7885480, ECO:0007744\|PDB:1IBR, ECO:0007744\|PDB:1K5D, ECO:0007744\|PDB:1QBK, ECO:0007744\|PDB:1RRP, ECO:0007744\|PDB:3GJX, ECO:0007744\|PDB:3NBY, ECO:0007744\|PDB:3NBZ, ECO:0007744\|PDB:3NC0, ECO:0007744\|PDB:3NC1, ECO:0007744\|PDB:3ZJY, ECO:0007744\|PDB:4C0Q, ECO:0007744\|PDB:4OL0, ECO:0007744\|PDB:5DH9, ECO:0007744\|PDB:5DHA, ECO:0007744\|PDB:5DHF, ECO:0007744\|PDB:5DI9, ECO:0007744\|PDB:5DIF, ECO:0007744\|PDB:5DIS, ECO:0007744\|PDB:5DLQ, ECO:0007744\|PDB:5JLJ, ECO:0007744\|PDB:5UWH, ECO:0007744\|PDB:5UWI, ECO:0007744\|PDB:5UWJ, ECO:0007744\|PDB:5UWO, ECO:0007744\|PDB:5UWP, ECO:0007744\|PDB:5UWQ, ECO:0007744\|PDB:5UWR, ECO:0007744\|PDB:5UWS, ECO:0007744\|PDB:5UWT, ECO:0007744\|PDB:5UWU, ECO:0007744\|PDB:5UWW

Chain	Residue	Details
A	GLY68

site_id	SWS_FT_FI4
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269\|PubMed:10078529, ECO:0000269\|PubMed:10353245, ECO:0000269\|PubMed:10367892, ECO:0000269\|PubMed:11832950, ECO:0000269\|PubMed:18611384, ECO:0000269\|PubMed:19389996, ECO:0000269\|PubMed:19505478, ECO:0000269\|PubMed:24449914, ECO:0000269\|PubMed:24915079, ECO:0000269\|PubMed:26272610, ECO:0000269\|PubMed:26349033, ECO:0000269\|PubMed:28282025, ECO:0000269\|PubMed:7885480, ECO:0007744\|PDB:1IBR, ECO:0007744\|PDB:1K5D, ECO:0007744\|PDB:1QBK, ECO:0007744\|PDB:1RRP, ECO:0007744\|PDB:3CH5, ECO:0007744\|PDB:3GJ0, ECO:0007744\|PDB:3GJ3, ECO:0007744\|PDB:3GJ4, ECO:0007744\|PDB:3GJ5, ECO:0007744\|PDB:3GJ6, ECO:0007744\|PDB:3GJ7, ECO:0007744\|PDB:3GJ8, ECO:0007744\|PDB:3GJX, ECO:0007744\|PDB:3NBY, ECO:0007744\|PDB:3NBZ, ECO:0007744\|PDB:3NC0, ECO:0007744\|PDB:3NC1, ECO:0007744\|PDB:3ZJY, ECO:0007744\|PDB:4C0Q, ECO:0007744\|PDB:4OL0, ECO:0007744\|PDB:5CIQ, ECO:0007744\|PDB:5CIT, ECO:0007744\|PDB:5CIW, ECO:0007744\|PDB:5CJ2, ECO:0007744\|PDB:5CLL, ECO:0007744\|PDB:5DH9, ECO:0007744\|PDB:5DHA, ECO:0007744\|PDB:5DHF, ECO:0007744\|PDB:5DI9, ECO:0007744\|PDB:5DIF, ECO:0007744\|PDB:5DIS, ECO:0007744\|PDB:5DLQ, ECO:0007744\|PDB:5JLJ, ECO:0007744\|PDB:5UWH, ECO:0007744\|PDB:5UWI, ECO:0007744\|PDB:5UWJ, ECO:0007744\|PDB:5UWO, ECO:0007744\|PDB:5UWP, ECO:0007744\|PDB:5UWQ, ECO:0007744\|PDB:5UWR, ECO:0007744\|PDB:5UWS, ECO:0007744\|PDB:5UWT, ECO:0007744\|PDB:5UWU, ECO:0007744\|PDB:5UWW

Chain	Residue	Details
A	ASN122

site_id	SWS_FT_FI5
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269\|PubMed:10078529, ECO:0000269\|PubMed:10353245, ECO:0000269\|PubMed:10367892, ECO:0000269\|PubMed:11832950, ECO:0000269\|PubMed:18611384, ECO:0000269\|PubMed:19389996, ECO:0000269\|PubMed:19505478, ECO:0000269\|PubMed:24449914, ECO:0000269\|PubMed:24915079, ECO:0000269\|PubMed:26272610, ECO:0000269\|PubMed:26349033, ECO:0000269\|PubMed:28282025, ECO:0000269\|PubMed:7885480, ECO:0007744\|PDB:1K5G, ECO:0007744\|PDB:3CH5, ECO:0007744\|PDB:3GJ0, ECO:0007744\|PDB:3GJ3, ECO:0007744\|PDB:3GJ4, ECO:0007744\|PDB:3GJ5, ECO:0007744\|PDB:3GJ6, ECO:0007744\|PDB:3GJ7, ECO:0007744\|PDB:3GJ8, ECO:0007744\|PDB:3GJX, ECO:0007744\|PDB:3NBY, ECO:0007744\|PDB:3NBZ, ECO:0007744\|PDB:3NC0, ECO:0007744\|PDB:3NC1, ECO:0007744\|PDB:3ZJY, ECO:0007744\|PDB:4C0Q, ECO:0007744\|PDB:4OL0, ECO:0007744\|PDB:5CIQ, ECO:0007744\|PDB:5CIT, ECO:0007744\|PDB:5CIW, ECO:0007744\|PDB:5CJ2, ECO:0007744\|PDB:5CLL, ECO:0007744\|PDB:5CLQ, ECO:0007744\|PDB:5DH9, ECO:0007744\|PDB:5DHA, ECO:0007744\|PDB:5DHF, ECO:0007744\|PDB:5DI9, ECO:0007744\|PDB:5DIF, ECO:0007744\|PDB:5DIS, ECO:0007744\|PDB:5DLQ, ECO:0007744\|PDB:5JLJ, ECO:0007744\|PDB:5UWH, ECO:0007744\|PDB:5UWI, ECO:0007744\|PDB:5UWJ, ECO:0007744\|PDB:5UWO, ECO:0007744\|PDB:5UWP, ECO:0007744\|PDB:5UWQ, ECO:0007744\|PDB:5UWR, ECO:0007744\|PDB:5UWS, ECO:0007744\|PDB:5UWT, ECO:0007744\|PDB:5UWU, ECO:0007744\|PDB:5UWW

Chain	Residue	Details
A	SER150

site_id	SWS_FT_FI6
Number of Residues	1
Details	SITE: Essential for GTP hydrolysis => ECO:0000269\|PubMed:18591255, ECO:0000269\|PubMed:26272610, ECO:0000269\|PubMed:8636225

Chain	Residue	Details
A	GLN69

site_id	SWS_FT_FI7
Number of Residues	1
Details	MOD_RES: N-acetylalanine => ECO:0000269\|Ref.12, ECO:0007744\|PubMed:19413330, ECO:0007744\|PubMed:22223895, ECO:0007744\|PubMed:22814378

Chain	Residue	Details
A	ALA2

site_id	SWS_FT_FI8
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:23186163

Chain	Residue	Details
A	THR24

site_id	SWS_FT_FI9
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0000269\|PubMed:31075303

Chain	Residue	Details
A	LYS37

site_id	SWS_FT_FI10
Number of Residues	2
Details	MOD_RES: N6-acetyllysine => ECO:0007744\|PubMed:19608861

Chain	Residue	Details
A	LYS60
A	LYS99

site_id	SWS_FT_FI11
Number of Residues	1
Details	MOD_RES: N6-acetyllysine; alternate => ECO:0007744\|PubMed:19608861

Chain	Residue	Details
A	LYS71

site_id	SWS_FT_FI12
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0000269\|PubMed:29040603

Chain	Residue	Details
A	LYS134

site_id	SWS_FT_FI13
Number of Residues	1
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000250\|UniProtKB:P62827

Chain	Residue	Details
A	LYS159

site_id	SWS_FT_FI14
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate

Chain	Residue	Details
A	LYS71

site_id	SWS_FT_FI15
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744\|PubMed:28112733

Chain	Residue	Details
A	LYS152

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)