Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4H6D

Crystal structure of PLP-soaked HMP synthase Thi5 from S. cerevisiae

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003674	molecular_function	molecular_function
A	0005506	molecular_function	iron ion binding
A	0005515	molecular_function	protein binding
A	0005575	cellular_component	cellular_component
A	0009228	biological_process	thiamine biosynthetic process
A	0009229	biological_process	thiamine diphosphate biosynthetic process
A	0016740	molecular_function	transferase activity
A	0046872	molecular_function	metal ion binding
A	0106344	molecular_function	4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase activity from histidine and PLP
B	0003674	molecular_function	molecular_function
B	0005506	molecular_function	iron ion binding
B	0005515	molecular_function	protein binding
B	0005575	cellular_component	cellular_component
B	0009228	biological_process	thiamine biosynthetic process
B	0009229	biological_process	thiamine diphosphate biosynthetic process
B	0016740	molecular_function	transferase activity
B	0046872	molecular_function	metal ion binding
B	0106344	molecular_function	4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase activity from histidine and PLP
C	0003674	molecular_function	molecular_function
C	0005506	molecular_function	iron ion binding
C	0005515	molecular_function	protein binding
C	0005575	cellular_component	cellular_component
C	0009228	biological_process	thiamine biosynthetic process
C	0009229	biological_process	thiamine diphosphate biosynthetic process
C	0016740	molecular_function	transferase activity
C	0046872	molecular_function	metal ion binding
C	0106344	molecular_function	4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase activity from histidine and PLP
D	0003674	molecular_function	molecular_function
D	0005506	molecular_function	iron ion binding
D	0005515	molecular_function	protein binding
D	0005575	cellular_component	cellular_component
D	0009228	biological_process	thiamine biosynthetic process
D	0009229	biological_process	thiamine diphosphate biosynthetic process
D	0016740	molecular_function	transferase activity
D	0046872	molecular_function	metal ion binding
D	0106344	molecular_function	4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase activity from histidine and PLP
E	0003674	molecular_function	molecular_function
E	0005506	molecular_function	iron ion binding
E	0005515	molecular_function	protein binding
E	0005575	cellular_component	cellular_component
E	0009228	biological_process	thiamine biosynthetic process
E	0009229	biological_process	thiamine diphosphate biosynthetic process
E	0016740	molecular_function	transferase activity
E	0046872	molecular_function	metal ion binding
E	0106344	molecular_function	4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase activity from histidine and PLP
F	0003674	molecular_function	molecular_function
F	0005506	molecular_function	iron ion binding
F	0005515	molecular_function	protein binding
F	0005575	cellular_component	cellular_component
F	0009228	biological_process	thiamine biosynthetic process
F	0009229	biological_process	thiamine diphosphate biosynthetic process
F	0016740	molecular_function	transferase activity
F	0046872	molecular_function	metal ion binding
F	0106344	molecular_function	4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase activity from histidine and PLP
G	0003674	molecular_function	molecular_function
G	0005506	molecular_function	iron ion binding
G	0005515	molecular_function	protein binding
G	0005575	cellular_component	cellular_component
G	0009228	biological_process	thiamine biosynthetic process
G	0009229	biological_process	thiamine diphosphate biosynthetic process
G	0016740	molecular_function	transferase activity
G	0046872	molecular_function	metal ion binding
G	0106344	molecular_function	4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase activity from histidine and PLP
H	0003674	molecular_function	molecular_function
H	0005506	molecular_function	iron ion binding
H	0005515	molecular_function	protein binding
H	0005575	cellular_component	cellular_component
H	0009228	biological_process	thiamine biosynthetic process
H	0009229	biological_process	thiamine diphosphate biosynthetic process
H	0016740	molecular_function	transferase activity
H	0046872	molecular_function	metal ion binding
H	0106344	molecular_function	4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase activity from histidine and PLP

Functional Information from PDB Data

site_id	AC1
Number of Residues	9
Details	BINDING SITE FOR RESIDUE PLP A 400

Chain	Residue
A	ASN11
A	PRO45
A	LYS62
A	HIS66
A	VAL114
A	GLY115
A	GLU116
A	PHE117
A	GLY118

site_id	AC2
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PLP B 400

Chain	Residue
B	ASN11
B	TRP12
B	PRO45
B	LYS62
B	HIS66
B	VAL114
B	GLY115
B	GLU116
B	PHE117
B	GLY118

site_id	AC3
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PLP E 400

Chain	Residue
E	ASN11
E	TRP12
E	PRO45
E	LYS62
E	HIS66
E	VAL114
E	GLY115
E	GLU116
E	PHE117
E	GLY118

site_id	AC4
Number of Residues	9
Details	BINDING SITE FOR RESIDUE PLP H 400

Chain	Residue
H	ASN11
H	PRO45
H	LYS62
H	HIS66
H	VAL114
H	GLY115
H	GLU116
H	PHE117
H	GLY118

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: ACT_SITE => ECO:0000305\|PubMed:23048037

Chain	Residue	Details
F	HIS66
A	HIS66
B	HIS66
C	HIS66
D	HIS66
E	HIS66
G	HIS66
H	HIS66

site_id	SWS_FT_FI2
Number of Residues	8
Details	BINDING: BINDING => ECO:0000269\|PubMed:23048037

Chain	Residue	Details
F	GLY115
A	GLY115
B	GLY115
C	GLY115
D	GLY115
E	GLY115
G	GLY115
H	GLY115

site_id	SWS_FT_FI3
Number of Residues	8
Details	MOD_RES: N6-(pyridoxal phosphate)lysine => ECO:0000269\|PubMed:23048037

Chain	Residue	Details
F	LYS62
A	LYS62
B	LYS62
C	LYS62
D	LYS62
E	LYS62
G	LYS62
H	LYS62

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)