4GUX

Crystal structure of trypsin:MCoTi-II complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004252	molecular_function	serine-type endopeptidase activity
A	0006508	biological_process	proteolysis
B	0004252	molecular_function	serine-type endopeptidase activity
B	0006508	biological_process	proteolysis
C	0004252	molecular_function	serine-type endopeptidase activity
C	0006508	biological_process	proteolysis
D	0004866	molecular_function	endopeptidase inhibitor activity
D	0004867	molecular_function	serine-type endopeptidase inhibitor activity
D	0005576	cellular_component	extracellular region
D	0006952	biological_process	defense response
D	0010466	biological_process	negative regulation of peptidase activity
D	1900004	biological_process	negative regulation of serine-type endopeptidase activity
E	0004866	molecular_function	endopeptidase inhibitor activity
E	0004867	molecular_function	serine-type endopeptidase inhibitor activity
E	0005576	cellular_component	extracellular region
E	0006952	biological_process	defense response
E	0010466	biological_process	negative regulation of peptidase activity
E	1900004	biological_process	negative regulation of serine-type endopeptidase activity
F	0004866	molecular_function	endopeptidase inhibitor activity
F	0004867	molecular_function	serine-type endopeptidase inhibitor activity
F	0005576	cellular_component	extracellular region
F	0006952	biological_process	defense response
F	0010466	biological_process	negative regulation of peptidase activity
F	1900004	biological_process	negative regulation of serine-type endopeptidase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 301

Chain	Residue
A	GLU72
A	ASN74
A	VAL77
A	GLU82
A	HOH544
A	HOH548

---

site_id	AC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE ACT A 302

Chain	Residue
A	HOH414
B	GLY24
B	LYS156
B	HOH449
A	GLY24
A	LYS156
A	HOH409

---

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA B 301

Chain	Residue
B	GLU72
B	ASN74
B	VAL77
B	GLU82
B	HOH564
B	HOH565

---

site_id	AC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE ACT B 302

Chain	Residue
B	TYR25
B	CYS27
B	THR31
B	LEU137
B	HOH545
B	HOH556
B	HOH631

---

site_id	AC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA C 301

Chain	Residue
C	GLU72
C	ASN74
C	VAL77
C	GLU82
C	HOH438
C	HOH580

---

Functional Information from PROSITE/UniProt

site_id	PS00134
Number of Residues	6
Details	TRYPSIN_HIS Serine proteases, trypsin family, histidine active site. VSAAHC

Chain	Residue	Details
A	VAL56-CYS61

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site_id	PS00135
Number of Residues	12
Details	TRYPSIN_SER Serine proteases, trypsin family, serine active site. DScqGDSGGPVV

Chain	Residue	Details
A	ASP191-VAL202

---

site_id	PS00286
Number of Residues	20
Details	SQUASH_INHIBITOR Squash family of serine protease inhibitors signature. CPkilkkCrrDsDCpgaCiC

Chain	Residue	Details
D	CYS8-CYS27

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	SITE: Reactive bond

Chain	Residue	Details
D	LYS10
E	LYS10
F	LYS10
B	HIS60
B	ASP104
B	SER197
C	HIS60
C	ASP104
C	SER197

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	CROSSLNK: Cyclopeptide (Ser-Gly) => ECO:0000269|PubMed:10801322

Chain	Residue	Details
D	SER1
B	VAL77
B	GLU82
B	ASP191
B	GLN194
B	SER197
C	GLU72
C	ASN74
C	VAL77
C	GLU82
C	ASP191
D	GLY34
C	GLN194
C	SER197
E	SER1
E	GLY34
F	SER1
F	GLY34
A	SER197
B	GLU72
B	ASN74

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	CROSSLNK: Isoaspartyl glycine isopeptide (Asp-Gly); alternate => ECO:0000269|PubMed:10801322

Chain	Residue	Details
D	ASP4
D	GLY5
E	ASP4
E	GLY5
F	ASP4
F	GLY5

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