Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4G7H

Crystal structure of Thermus thermophilus transcription initiation complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003899	molecular_function	DNA-directed RNA polymerase activity
A	0005515	molecular_function	protein binding
A	0006351	biological_process	DNA-templated transcription
A	0034062	molecular_function	5'-3' RNA polymerase activity
A	0046983	molecular_function	protein dimerization activity
B	0003677	molecular_function	DNA binding
B	0003899	molecular_function	DNA-directed RNA polymerase activity
B	0005515	molecular_function	protein binding
B	0006351	biological_process	DNA-templated transcription
B	0034062	molecular_function	5'-3' RNA polymerase activity
B	0046983	molecular_function	protein dimerization activity
C	0003677	molecular_function	DNA binding
C	0003899	molecular_function	DNA-directed RNA polymerase activity
C	0006351	biological_process	DNA-templated transcription
C	0032549	molecular_function	ribonucleoside binding
C	0034062	molecular_function	5'-3' RNA polymerase activity
D	0000287	molecular_function	magnesium ion binding
D	0003677	molecular_function	DNA binding
D	0003899	molecular_function	DNA-directed RNA polymerase activity
D	0005515	molecular_function	protein binding
D	0006351	biological_process	DNA-templated transcription
D	0008270	molecular_function	zinc ion binding
D	0034062	molecular_function	5'-3' RNA polymerase activity
E	0003677	molecular_function	DNA binding
E	0003899	molecular_function	DNA-directed RNA polymerase activity
E	0006351	biological_process	DNA-templated transcription
E	0034062	molecular_function	5'-3' RNA polymerase activity
F	0003677	molecular_function	DNA binding
F	0003700	molecular_function	DNA-binding transcription factor activity
F	0005737	cellular_component	cytoplasm
F	0006352	biological_process	DNA-templated transcription initiation
F	0006355	biological_process	regulation of DNA-templated transcription
F	0010468	biological_process	regulation of gene expression
F	0016987	molecular_function	sigma factor activity
F	2000142	biological_process	regulation of DNA-templated transcription initiation
K	0003677	molecular_function	DNA binding
K	0003899	molecular_function	DNA-directed RNA polymerase activity
K	0005515	molecular_function	protein binding
K	0006351	biological_process	DNA-templated transcription
K	0034062	molecular_function	5'-3' RNA polymerase activity
K	0046983	molecular_function	protein dimerization activity
L	0003677	molecular_function	DNA binding
L	0003899	molecular_function	DNA-directed RNA polymerase activity
L	0005515	molecular_function	protein binding
L	0006351	biological_process	DNA-templated transcription
L	0034062	molecular_function	5'-3' RNA polymerase activity
L	0046983	molecular_function	protein dimerization activity
M	0003677	molecular_function	DNA binding
M	0003899	molecular_function	DNA-directed RNA polymerase activity
M	0006351	biological_process	DNA-templated transcription
M	0032549	molecular_function	ribonucleoside binding
M	0034062	molecular_function	5'-3' RNA polymerase activity
N	0000287	molecular_function	magnesium ion binding
N	0003677	molecular_function	DNA binding
N	0003899	molecular_function	DNA-directed RNA polymerase activity
N	0005515	molecular_function	protein binding
N	0006351	biological_process	DNA-templated transcription
N	0008270	molecular_function	zinc ion binding
N	0034062	molecular_function	5'-3' RNA polymerase activity
O	0003677	molecular_function	DNA binding
O	0003899	molecular_function	DNA-directed RNA polymerase activity
O	0006351	biological_process	DNA-templated transcription
O	0034062	molecular_function	5'-3' RNA polymerase activity
P	0003677	molecular_function	DNA binding
P	0003700	molecular_function	DNA-binding transcription factor activity
P	0005737	cellular_component	cytoplasm
P	0006352	biological_process	DNA-templated transcription initiation
P	0006355	biological_process	regulation of DNA-templated transcription
P	0010468	biological_process	regulation of gene expression
P	0016987	molecular_function	sigma factor activity
P	2000142	biological_process	regulation of DNA-templated transcription initiation

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG B 2001

Chain	Residue
B	ASP183
B	ASP191
B	LEU192
B	ASP193

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN D 2001

Chain	Residue
D	CYS1112
D	CYS1194
D	CYS1201
D	CYS1204

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN D 2002

Chain	Residue
D	CYS60
D	CYS73
D	CYS76
D	CYS58

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG D 2003

Chain	Residue
D	ASP739
D	ASP741
D	ASP743

site_id	AC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG D 2004

Chain	Residue
B	GLU154
B	ASP168
D	LYS840

site_id	AC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG D 2005

Chain	Residue
D	TRP897
D	GLU898
D	ILE900

site_id	AC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG F 2001

Chain	Residue
F	ALA292
F	GLY296
F	TRP299

site_id	AC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG K 1001

Chain	Residue
K	THR51
K	VAL170
K	PHE171
K	SER172
K	ASP202

site_id	AC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN N 2001

Chain	Residue
N	CYS1112
N	CYS1194
N	CYS1201
N	CYS1204

site_id	BC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN N 2002

Chain	Residue
N	CYS58
N	CYS60
N	CYS73
N	CYS76

site_id	BC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG N 2003

Chain	Residue
N	ASP739
N	ASP741
N	ASP743

site_id	BC3
Number of Residues	1
Details	BINDING SITE FOR RESIDUE MG N 2004

Chain	Residue
N	LYS840

site_id	BC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG N 2005

Chain	Residue
N	TRP897
N	GLU898
N	ILE900

site_id	BC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG P 2001

Chain	Residue
P	ALA292
P	GLY296
P	TRP299

Functional Information from PROSITE/UniProt

site_id	PS00716
Number of Residues	27
Details	SIGMA70_2 Sigma-70 factors family signature 2. TleEVGaffgVTrerIrQIEnkaLrkL

Chain	Residue	Details
F	THR382-LEU408

site_id	PS00867
Number of Residues	8
Details	CPSASE_2 Carbamoyl-phosphate synthase subdomain signature 2. LIEANLRL

Chain	Residue	Details
F	LEU187-LEU194

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01322","evidenceCode":"ECO:0000255"},{"source":"PDB","id":"8HSR","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01322","evidenceCode":"ECO:0000255"},{"source":"PDB","id":"3AOI","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HSG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HSH","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HSL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HSR","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01322","evidenceCode":"ECO:0000255"},{"source":"PDB","id":"3AOH","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HSG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HSH","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HSL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HSR","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01322","evidenceCode":"ECO:0000255"},{"source":"PDB","id":"8HSG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HSH","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HSL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HSR","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01322","evidenceCode":"ECO:0000255"},{"source":"PDB","id":"3AOH","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3AOI","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HSG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HSH","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HSL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8HSR","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)