4G7H
Crystal structure of Thermus thermophilus transcription initiation complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-03-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 185.560, 104.570, 297.550 |
Unit cell angles | 90.00, 98.32, 90.00 |
Refinement procedure
Resolution | 49.270 - 2.900 |
R-factor | 0.1884 |
Rwork | 0.188 |
R-free | 0.22590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iw7 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.989 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.050 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.101 | 0.667 |
Number of reflections | 249337 | |
<I/σ(I)> | 11.2 | 2.3 |
Completeness [%] | 99.6 | 99.1 |
Redundancy | 4.9 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 295 | 100 mM Tris-HCl, pH 8.7, 200 mM potassium chloride, 50 mM magnesium chloride, 10% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |