Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4EBF

SeMet thermostable phosphite dehydrogenase Glu175-Ala mutant

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005829	cellular_component	cytosol
A	0008465	molecular_function	hydroxypyruvate reductase (NADH) activity
A	0016491	molecular_function	oxidoreductase activity
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0016618	molecular_function	hydroxypyruvate reductase [NAD(P)H] activity
A	0030267	molecular_function	glyoxylate reductase (NADPH) activity
A	0050609	molecular_function	phosphonate dehydrogenase activity
A	0051287	molecular_function	NAD binding
B	0005829	cellular_component	cytosol
B	0008465	molecular_function	hydroxypyruvate reductase (NADH) activity
B	0016491	molecular_function	oxidoreductase activity
B	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
B	0016618	molecular_function	hydroxypyruvate reductase [NAD(P)H] activity
B	0030267	molecular_function	glyoxylate reductase (NADPH) activity
B	0050609	molecular_function	phosphonate dehydrogenase activity
B	0051287	molecular_function	NAD binding
C	0005829	cellular_component	cytosol
C	0008465	molecular_function	hydroxypyruvate reductase (NADH) activity
C	0016491	molecular_function	oxidoreductase activity
C	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
C	0016618	molecular_function	hydroxypyruvate reductase [NAD(P)H] activity
C	0030267	molecular_function	glyoxylate reductase (NADPH) activity
C	0050609	molecular_function	phosphonate dehydrogenase activity
C	0051287	molecular_function	NAD binding
D	0005829	cellular_component	cytosol
D	0008465	molecular_function	hydroxypyruvate reductase (NADH) activity
D	0016491	molecular_function	oxidoreductase activity
D	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
D	0016618	molecular_function	hydroxypyruvate reductase [NAD(P)H] activity
D	0030267	molecular_function	glyoxylate reductase (NADPH) activity
D	0050609	molecular_function	phosphonate dehydrogenase activity
D	0051287	molecular_function	NAD binding
E	0005829	cellular_component	cytosol
E	0008465	molecular_function	hydroxypyruvate reductase (NADH) activity
E	0016491	molecular_function	oxidoreductase activity
E	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
E	0016618	molecular_function	hydroxypyruvate reductase [NAD(P)H] activity
E	0030267	molecular_function	glyoxylate reductase (NADPH) activity
E	0050609	molecular_function	phosphonate dehydrogenase activity
E	0051287	molecular_function	NAD binding
F	0005829	cellular_component	cytosol
F	0008465	molecular_function	hydroxypyruvate reductase (NADH) activity
F	0016491	molecular_function	oxidoreductase activity
F	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
F	0016618	molecular_function	hydroxypyruvate reductase [NAD(P)H] activity
F	0030267	molecular_function	glyoxylate reductase (NADPH) activity
F	0050609	molecular_function	phosphonate dehydrogenase activity
F	0051287	molecular_function	NAD binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	22
Details	BINDING SITE FOR RESIDUE NAD A 800

Chain	Residue
A	LYS76
A	LEU208
A	PRO209
A	PRO235
A	CYS236
A	ARG237
A	ASP261
A	HIS292
A	GLY294
A	HOH902
A	HOH909
A	THR104
A	HOH920
A	HOH958
A	HOH969
A	MSE153
A	GLY154
A	ALA155
A	ILE156
A	ALA175
A	ALA176
A	LYS177

site_id	AC2
Number of Residues	25
Details	BINDING SITE FOR RESIDUE NAD B 800

Chain	Residue
B	LYS76
B	GLY77
B	THR104
B	MSE153
B	GLY154
B	ALA155
B	ILE156
B	ALA175
B	ALA176
B	ALA207
B	LEU208
B	PRO209
B	PRO235
B	CYS236
B	ARG237
B	ASP261
B	HIS292
B	GLY294
B	HOH902
B	HOH913
B	HOH932
B	HOH936
B	HOH977
B	HOH1016
B	HOH1019

site_id	AC3
Number of Residues	22
Details	BINDING SITE FOR RESIDUE NAD C 800

Chain	Residue
C	THR104
C	GLY152
C	MSE153
C	GLY154
C	ALA155
C	ILE156
C	ALA175
C	ALA176
C	ALA207
C	LEU208
C	PRO209
C	THR214
C	PRO235
C	CYS236
C	ASP261
C	HIS292
C	GLY294
C	HOH913
C	HOH934
C	HOH940
C	HOH967
C	HOH998

site_id	AC4
Number of Residues	20
Details	BINDING SITE FOR RESIDUE NAD D 800

Chain	Residue
D	LYS76
D	GLY77
D	THR104
D	MSE153
D	GLY154
D	ALA155
D	ILE156
D	ALA176
D	LEU208
D	PRO209
D	PRO235
D	CYS236
D	ARG237
D	ASP261
D	VAL262
D	HIS292
D	GLY294
D	HOH903
D	HOH921
D	HOH986

Functional Information from PROSITE/UniProt

site_id	PS00671
Number of Residues	17
Details	D_2_HYDROXYACID_DH_3 D-isomer specific 2-hydroxyacid dehydrogenases signature 3. VRpGaLLVNpCRGsVVD

Chain	Residue	Details
A	VAL226-ASP242

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	ACT_SITE: ACT_SITE => ECO:0000250

Chain	Residue	Details
A	ARG237
E	GLU266
F	ARG237
F	GLU266
A	GLU266
B	ARG237
B	GLU266
C	ARG237
C	GLU266
D	ARG237
D	GLU266
E	ARG237

site_id	SWS_FT_FI2
Number of Residues	6
Details	ACT_SITE: Proton donor => ECO:0000250

Chain	Residue	Details
A	HIS292
B	HIS292
C	HIS292
D	HIS292
E	HIS292
F	HIS292

site_id	SWS_FT_FI3
Number of Residues	30
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
A	ALA155
B	HIS292
C	ALA155
C	ALA175
C	PRO235
C	ASP261
C	HIS292
D	ALA155
D	ALA175
D	PRO235
D	ASP261
A	ALA175
D	HIS292
E	ALA155
E	ALA175
E	PRO235
E	ASP261
E	HIS292
F	ALA155
F	ALA175
F	PRO235
F	ASP261
A	PRO235
F	HIS292
A	ASP261
A	HIS292
B	ALA155
B	ALA175
B	PRO235
B	ASP261

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)