4EBF
SeMet thermostable phosphite dehydrogenase Glu175-Ala mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.218, 122.580, 135.015 |
| Unit cell angles | 90.00, 96.36, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.300 |
| R-factor | 0.21066 |
| Rwork | 0.207 |
| R-free | 0.27361 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.482 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 98877 | |
| <I/σ(I)> | 19.4 | 3.9 |
| Completeness [%] | 98.9 | 98.3 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 25-30% PEG 3350, 100 mM KCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
