4E3Y
X-ray structure of the Serratia marcescens endonuclease at 0.95 A resolution
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003676 | molecular_function | nucleic acid binding |
| A | 0004519 | molecular_function | endonuclease activity |
| A | 0005576 | cellular_component | extracellular region |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0046872 | molecular_function | metal ion binding |
| B | 0003676 | molecular_function | nucleic acid binding |
| B | 0004519 | molecular_function | endonuclease activity |
| B | 0005576 | cellular_component | extracellular region |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG A 2601 |
| Chain | Residue |
| A | ASN1119 |
| A | HOH2701 |
| A | HOH2702 |
| A | HOH2703 |
| A | HOH2704 |
| A | HOH2705 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE EDO A 2602 |
| Chain | Residue |
| A | HOH2729 |
| B | ASP2208 |
| B | LYS2212 |
| A | ALA1169 |
| A | ASP1191 |
| A | ARG1213 |
| site_id | AC3 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE PEG A 2603 |
| Chain | Residue |
| A | THR1035 |
| A | LYS1037 |
| A | LYS1162 |
| A | ALA1163 |
| A | HOH2781 |
| A | HOH2782 |
| A | HOH2979 |
| A | HOH3023 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG B 2301 |
| Chain | Residue |
| B | ASN2119 |
| B | HOH2401 |
| B | HOH2402 |
| B | HOH2403 |
| B | HOH2404 |
| B | HOH2405 |
| site_id | AC5 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE SO4 B 2302 |
| Chain | Residue |
| A | ASN1020 |
| B | GLY2054 |
| B | ARG2057 |
| B | ALA2094 |
| B | HOH2475 |
| B | HOH2592 |
| B | HOH2593 |
| B | HOH2630 |
| B | HOH2684 |
| site_id | AC6 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE SO4 B 2303 |
| Chain | Residue |
| A | ASN1020 |
| B | ARG2057 |
| B | ARG2087 |
| B | HIS2089 |
| B | ASN2119 |
| B | HOH2401 |
| B | HOH2402 |
| B | HOH2572 |
| B | HOH2712 |
| B | HOH2735 |
| site_id | AC7 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL B 2304 |
| Chain | Residue |
| B | THR2077 |
| B | GLY2078 |
| B | ARG2087 |
| B | HOH2517 |
| B | HOH2704 |
| site_id | AC8 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE EDO B 2305 |
| Chain | Residue |
| B | PRO2051 |
| B | ARG2131 |
| B | ILE2134 |
| B | ASP2135 |
Functional Information from PROSITE/UniProt
| site_id | PS01070 |
| Number of Residues | 9 |
| Details | NUCLEASE_NON_SPEC DNA/RNA non-specific endonucleases active site. DRGHQaplA |
| Chain | Residue | Details |
| A | ASP1086-ALA1094 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton acceptor => ECO:0000255|PROSITE-ProRule:PRU10047, ECO:0000269|PubMed:8078761 |
| Chain | Residue | Details |
| A | HIS1089 | |
| B | HIS2089 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | BINDING: |
| Chain | Residue | Details |
| A | ASN1119 | |
| B | ASN2119 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 5 |
| Details | M-CSA 42 |
| Chain | Residue | Details |
| A | ARG1057 | electrostatic stabiliser |
| A | HIS1089 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| A | ASN1110 | electrostatic stabiliser, increase acidity, increase basicity |
| A | ASN1119 | activator, hydrogen bond acceptor, hydrogen bond donor, metal ligand |
| A | GLU1127 | electrostatic stabiliser, hydrogen bond acceptor, metal ligand, proton acceptor, proton donor |
| site_id | MCSA2 |
| Number of Residues | 5 |
| Details | M-CSA 42 |
| Chain | Residue | Details |
| B | ARG2057 | electrostatic stabiliser |
| B | HIS2089 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| B | ASN2110 | electrostatic stabiliser, increase acidity, increase basicity |
| B | ASN2119 | activator, hydrogen bond acceptor, hydrogen bond donor, metal ligand |
| B | GLU2127 | electrostatic stabiliser, hydrogen bond acceptor, metal ligand, proton acceptor, proton donor |
