4E3Y
X-ray structure of the Serratia marcescens endonuclease at 0.95 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-10-27 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.847 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 106.265, 73.418, 67.924 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 0.950 |
R-factor | 0.1309 |
Rwork | 0.130 |
R-free | 0.14530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g8t |
RMSD bond length | 0.017 |
RMSD bond angle | 1.820 |
Data reduction software | DENZO |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 27.597 |
High resolution limit [Å] | 0.950 |
Number of reflections | 313007 |
Completeness [%] | 94.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 298 | 10 mM TRIS-HCl, 1 mM Mg(2+), PEG4000, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K |